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Stimulation of proinflammatory cytokines by volatile sulfur compounds in endodontically treated teeth.

Lechner J, von Baehr V - Int J Gen Med (2015)

Bottom Line: Persistent microorganisms in endodontically treated teeth produce volatile sulfur compounds (VSC) such as methyl mercaptan, hydrogen sulfide, and thioether.Data elucidate the role of VSC in patients with immunologic diseases and the role of a semiquantitative chairside test, like the VSC indicator presented here, in correlation to IFNg and IL-10 sensitization in peripheral blood mononuclear cells.The association between ex vivo-stimulated cytokines and endodontically derived sulfur components is supported by the fact that the number of interferon gamma- and/or interleukin-10-positive sensitized patients declined significantly 3-8 months after extraction of the corresponding teeth.

View Article: PubMed Central - PubMed

Affiliation: Clinic for Integrative Dentistry, Munich, Germany.

ABSTRACT
Persistent microorganisms in endodontically treated teeth produce volatile sulfur compounds (VSC) such as methyl mercaptan, hydrogen sulfide, and thioether. In this retrospective study, we evaluated the ex vivo immune response of peripheral blood mononuclear cells to sulfur compounds in 354 patients with systemic diseases. These systemic findings are correlated with semiquantitative values of a VSC indicator applied directly on endodontically treated teeth. Data elucidate the role of VSC in patients with immunologic diseases and the role of a semiquantitative chairside test, like the VSC indicator presented here, in correlation to IFNg and IL-10 sensitization in peripheral blood mononuclear cells. The association between ex vivo-stimulated cytokines and endodontically derived sulfur components is supported by the fact that the number of interferon gamma- and/or interleukin-10-positive sensitized patients declined significantly 3-8 months after extraction of the corresponding teeth.

No MeSH data available.


Related in: MedlinePlus

Distribution of study group based on patients’ sensitization to Merc/Thio exposure.Notes: Positive sensitization is sensitization to IFNg and/or IL-10, and negative sensitization is no sensitization to IFNg and/or IL-10 as a result of exposure to Merc/Thio. IFNg and IL-10 levels estimated in ex vivo PBMC cultures and serum samples from patients.Abbreviations: Merc/Thio, mercaptan and thioether; PBMC, peripheral blood mononuclear cells; IFNg, interferon gamma.
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f2-ijgm-8-109: Distribution of study group based on patients’ sensitization to Merc/Thio exposure.Notes: Positive sensitization is sensitization to IFNg and/or IL-10, and negative sensitization is no sensitization to IFNg and/or IL-10 as a result of exposure to Merc/Thio. IFNg and IL-10 levels estimated in ex vivo PBMC cultures and serum samples from patients.Abbreviations: Merc/Thio, mercaptan and thioether; PBMC, peripheral blood mononuclear cells; IFNg, interferon gamma.

Mentions: Effector cell typing in the laboratory is used to detect derailed immune reactions caused by VSC in the patient’s serum. The degree of immunological sensitization is defined by the level of IFNg and IL-10 in terms of IU/mL. In 2011 and 2012, we had obtained serum from a total of 354 patients for Merc/Thio effector cell typing. When defining a positive result of the ex vivo stimulation assay as IFNg >0.3 IU/mL and/or IL-10 >10 IU/mL, 182 of the 354 patients examined (51%) scored positive, while 172 scored negative, as a result of exposure to Merc/Thio. Of the 354 individuals examined, 182 showed sensitization to IFNg and/or IL-10 as a result of exposure to Merc/Thio and 172 individuals showed no sensitization to IFNg and/or IL-10. Figure 2 shows a graphical representation of the distribution: one in two patients with RFT showed IFNg- and/or IL-10-based sensitization to Merc/Thio. The distribution of the IFNg/IL-10 constellations within the group of patients exhibiting sensitization indicates that 61 of the 182 patients are only sensitized to IFNg, and that 100 of the 182 patients are sensitized only to IL-10; 21 patients reacted to both concurrently.


Stimulation of proinflammatory cytokines by volatile sulfur compounds in endodontically treated teeth.

Lechner J, von Baehr V - Int J Gen Med (2015)

Distribution of study group based on patients’ sensitization to Merc/Thio exposure.Notes: Positive sensitization is sensitization to IFNg and/or IL-10, and negative sensitization is no sensitization to IFNg and/or IL-10 as a result of exposure to Merc/Thio. IFNg and IL-10 levels estimated in ex vivo PBMC cultures and serum samples from patients.Abbreviations: Merc/Thio, mercaptan and thioether; PBMC, peripheral blood mononuclear cells; IFNg, interferon gamma.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4362895&req=5

f2-ijgm-8-109: Distribution of study group based on patients’ sensitization to Merc/Thio exposure.Notes: Positive sensitization is sensitization to IFNg and/or IL-10, and negative sensitization is no sensitization to IFNg and/or IL-10 as a result of exposure to Merc/Thio. IFNg and IL-10 levels estimated in ex vivo PBMC cultures and serum samples from patients.Abbreviations: Merc/Thio, mercaptan and thioether; PBMC, peripheral blood mononuclear cells; IFNg, interferon gamma.
Mentions: Effector cell typing in the laboratory is used to detect derailed immune reactions caused by VSC in the patient’s serum. The degree of immunological sensitization is defined by the level of IFNg and IL-10 in terms of IU/mL. In 2011 and 2012, we had obtained serum from a total of 354 patients for Merc/Thio effector cell typing. When defining a positive result of the ex vivo stimulation assay as IFNg >0.3 IU/mL and/or IL-10 >10 IU/mL, 182 of the 354 patients examined (51%) scored positive, while 172 scored negative, as a result of exposure to Merc/Thio. Of the 354 individuals examined, 182 showed sensitization to IFNg and/or IL-10 as a result of exposure to Merc/Thio and 172 individuals showed no sensitization to IFNg and/or IL-10. Figure 2 shows a graphical representation of the distribution: one in two patients with RFT showed IFNg- and/or IL-10-based sensitization to Merc/Thio. The distribution of the IFNg/IL-10 constellations within the group of patients exhibiting sensitization indicates that 61 of the 182 patients are only sensitized to IFNg, and that 100 of the 182 patients are sensitized only to IL-10; 21 patients reacted to both concurrently.

Bottom Line: Persistent microorganisms in endodontically treated teeth produce volatile sulfur compounds (VSC) such as methyl mercaptan, hydrogen sulfide, and thioether.Data elucidate the role of VSC in patients with immunologic diseases and the role of a semiquantitative chairside test, like the VSC indicator presented here, in correlation to IFNg and IL-10 sensitization in peripheral blood mononuclear cells.The association between ex vivo-stimulated cytokines and endodontically derived sulfur components is supported by the fact that the number of interferon gamma- and/or interleukin-10-positive sensitized patients declined significantly 3-8 months after extraction of the corresponding teeth.

View Article: PubMed Central - PubMed

Affiliation: Clinic for Integrative Dentistry, Munich, Germany.

ABSTRACT
Persistent microorganisms in endodontically treated teeth produce volatile sulfur compounds (VSC) such as methyl mercaptan, hydrogen sulfide, and thioether. In this retrospective study, we evaluated the ex vivo immune response of peripheral blood mononuclear cells to sulfur compounds in 354 patients with systemic diseases. These systemic findings are correlated with semiquantitative values of a VSC indicator applied directly on endodontically treated teeth. Data elucidate the role of VSC in patients with immunologic diseases and the role of a semiquantitative chairside test, like the VSC indicator presented here, in correlation to IFNg and IL-10 sensitization in peripheral blood mononuclear cells. The association between ex vivo-stimulated cytokines and endodontically derived sulfur components is supported by the fact that the number of interferon gamma- and/or interleukin-10-positive sensitized patients declined significantly 3-8 months after extraction of the corresponding teeth.

No MeSH data available.


Related in: MedlinePlus