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In vitro gastrointestinal digestion study of two wheat cultivars and evaluation of xylanase supplementation.

Lafond M, Bouza B, Eyrichine S, Rouffineau F, Saulnier L, Giardina T, Bonnin E, Preynat A - J Anim Sci Biotechnol (2015)

Bottom Line: In order to appreciate their in vivo mode of action, the supplementation effect of two of its xylanases, XynD and XynB from families GH10 and GH11 respectively, have been evaluated on two different wheat cultivars Caphorn and Isengrain, which were chosen amongst 6 varieties for their difference in non starch polysaccharides content and arabinoxylan composition.Polysaccharide degradation appeared to occur mainly at the jejunal level and was higher with Isengrain than with Caphorn.For both cultivars, XynD and XynB supplementation increased notably the amount of reducing end sugars into the jejuno-ileal dialysates, which has been confirmed by a valuable increase of the soluble glucose into the jejunal dialysates.

View Article: PubMed Central - PubMed

Affiliation: iSm2 - BiosCiences UMR 7313, Aix Marseille Université, Centrale Marseille, CNRS, Marseille, France.

ABSTRACT

Background: The filamentous fungus Talaromyces versatilis is known to improve the metabolizable energy of wheat-based poultry diets thanks to its ability to produce a pool of CAZymes and particularly endo-β(1,4)-xylanases. In order to appreciate their in vivo mode of action, the supplementation effect of two of its xylanases, XynD and XynB from families GH10 and GH11 respectively, have been evaluated on two different wheat cultivars Caphorn and Isengrain, which were chosen amongst 6 varieties for their difference in non starch polysaccharides content and arabinoxylan composition.

Results: Polysaccharides digestion was followed during 6 h along the digestive tract using the TNO gastrointestinal model-1, to mimic monogastric metabolism. Polysaccharide degradation appeared to occur mainly at the jejunal level and was higher with Isengrain than with Caphorn. For both cultivars, XynD and XynB supplementation increased notably the amount of reducing end sugars into the jejuno-ileal dialysates, which has been confirmed by a valuable increase of the soluble glucose into the jejunal dialysates.

Conclusions: The amounts of arabinose and xylose into the dialysates and ileal deliveries increased consequently mainly for Caphorn, suggesting that XynD and XynB supplementation in wheat-based diet could alleviate the anti-nutritional effects of arabinoxylans by limiting the physical entrapment of starch and could increase the available metabolizable energy.

No MeSH data available.


XynD and XynB supplementation effects on Caphorn and Isengrain cultivars in the TIM-1 compartments. The xylanase supplementation effects on Caphorn and Isengrain with XynD (full light grey and full dark grey) and with XynB (hatched light grey and hatched dark grey) were expressed in percentage of soluble arabinose (upper), xylose (medium) and glucose (lower) increase or decrease compared to the control without enzyme addition. *Corresponds to the significant difference.
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Fig4: XynD and XynB supplementation effects on Caphorn and Isengrain cultivars in the TIM-1 compartments. The xylanase supplementation effects on Caphorn and Isengrain with XynD (full light grey and full dark grey) and with XynB (hatched light grey and hatched dark grey) were expressed in percentage of soluble arabinose (upper), xylose (medium) and glucose (lower) increase or decrease compared to the control without enzyme addition. *Corresponds to the significant difference.

Mentions: In order to get an overview of the effect of xylanase supplementation on monosaccharide content for each of the wheat cultivars, the values obtained for each monosaccharide in each compartment have been pooled together to define both the gastro-intestinal residues, and the jejuno-ileal dialysates (Figure 4). The enzyme effect was expressed as the percent increase or decrease of the monosaccharide release compared to the control without enzyme. XynD and XynB additions significantly affected the contents in monosaccharide in all the samples collected after 360 min of digestion. XynD increased the arabinose and xylose contents in the different fractions and for both wheat cultivars. Specifically, XynD supplementation increased the arabinose amount for Caphorn and Isengrain with +14.5% and +25.6% into the jejuno-ileal dialysates, +14.1% and +26.8% into the ileal deliveries and +28.9% and +30.0% into the gastro-intestinal residues, respectively. The xylose content in the presence of XynD followed the increase of the arabinose amount with for Caphorn and Isengrain, +78.7% and +115.0% into the jejuno-ileal dialysates, +13.9% and +23.2% into the ileal deliveries and +27.7% and +32.3% into the gastro-intestinal residues, respectively. Finally, there was a trend for an increase of the glucose content for both wheat cultivars in the TIM-1 fractions collected in the presence of XynD. The largest increase of glucose content was observed on Isengrain cultivar into the jejuno-ileal dialysate (+39.7%).Figure 4


In vitro gastrointestinal digestion study of two wheat cultivars and evaluation of xylanase supplementation.

Lafond M, Bouza B, Eyrichine S, Rouffineau F, Saulnier L, Giardina T, Bonnin E, Preynat A - J Anim Sci Biotechnol (2015)

XynD and XynB supplementation effects on Caphorn and Isengrain cultivars in the TIM-1 compartments. The xylanase supplementation effects on Caphorn and Isengrain with XynD (full light grey and full dark grey) and with XynB (hatched light grey and hatched dark grey) were expressed in percentage of soluble arabinose (upper), xylose (medium) and glucose (lower) increase or decrease compared to the control without enzyme addition. *Corresponds to the significant difference.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4362821&req=5

Fig4: XynD and XynB supplementation effects on Caphorn and Isengrain cultivars in the TIM-1 compartments. The xylanase supplementation effects on Caphorn and Isengrain with XynD (full light grey and full dark grey) and with XynB (hatched light grey and hatched dark grey) were expressed in percentage of soluble arabinose (upper), xylose (medium) and glucose (lower) increase or decrease compared to the control without enzyme addition. *Corresponds to the significant difference.
Mentions: In order to get an overview of the effect of xylanase supplementation on monosaccharide content for each of the wheat cultivars, the values obtained for each monosaccharide in each compartment have been pooled together to define both the gastro-intestinal residues, and the jejuno-ileal dialysates (Figure 4). The enzyme effect was expressed as the percent increase or decrease of the monosaccharide release compared to the control without enzyme. XynD and XynB additions significantly affected the contents in monosaccharide in all the samples collected after 360 min of digestion. XynD increased the arabinose and xylose contents in the different fractions and for both wheat cultivars. Specifically, XynD supplementation increased the arabinose amount for Caphorn and Isengrain with +14.5% and +25.6% into the jejuno-ileal dialysates, +14.1% and +26.8% into the ileal deliveries and +28.9% and +30.0% into the gastro-intestinal residues, respectively. The xylose content in the presence of XynD followed the increase of the arabinose amount with for Caphorn and Isengrain, +78.7% and +115.0% into the jejuno-ileal dialysates, +13.9% and +23.2% into the ileal deliveries and +27.7% and +32.3% into the gastro-intestinal residues, respectively. Finally, there was a trend for an increase of the glucose content for both wheat cultivars in the TIM-1 fractions collected in the presence of XynD. The largest increase of glucose content was observed on Isengrain cultivar into the jejuno-ileal dialysate (+39.7%).Figure 4

Bottom Line: In order to appreciate their in vivo mode of action, the supplementation effect of two of its xylanases, XynD and XynB from families GH10 and GH11 respectively, have been evaluated on two different wheat cultivars Caphorn and Isengrain, which were chosen amongst 6 varieties for their difference in non starch polysaccharides content and arabinoxylan composition.Polysaccharide degradation appeared to occur mainly at the jejunal level and was higher with Isengrain than with Caphorn.For both cultivars, XynD and XynB supplementation increased notably the amount of reducing end sugars into the jejuno-ileal dialysates, which has been confirmed by a valuable increase of the soluble glucose into the jejunal dialysates.

View Article: PubMed Central - PubMed

Affiliation: iSm2 - BiosCiences UMR 7313, Aix Marseille Université, Centrale Marseille, CNRS, Marseille, France.

ABSTRACT

Background: The filamentous fungus Talaromyces versatilis is known to improve the metabolizable energy of wheat-based poultry diets thanks to its ability to produce a pool of CAZymes and particularly endo-β(1,4)-xylanases. In order to appreciate their in vivo mode of action, the supplementation effect of two of its xylanases, XynD and XynB from families GH10 and GH11 respectively, have been evaluated on two different wheat cultivars Caphorn and Isengrain, which were chosen amongst 6 varieties for their difference in non starch polysaccharides content and arabinoxylan composition.

Results: Polysaccharides digestion was followed during 6 h along the digestive tract using the TNO gastrointestinal model-1, to mimic monogastric metabolism. Polysaccharide degradation appeared to occur mainly at the jejunal level and was higher with Isengrain than with Caphorn. For both cultivars, XynD and XynB supplementation increased notably the amount of reducing end sugars into the jejuno-ileal dialysates, which has been confirmed by a valuable increase of the soluble glucose into the jejunal dialysates.

Conclusions: The amounts of arabinose and xylose into the dialysates and ileal deliveries increased consequently mainly for Caphorn, suggesting that XynD and XynB supplementation in wheat-based diet could alleviate the anti-nutritional effects of arabinoxylans by limiting the physical entrapment of starch and could increase the available metabolizable energy.

No MeSH data available.