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In vitro gastrointestinal digestion study of two wheat cultivars and evaluation of xylanase supplementation.

Lafond M, Bouza B, Eyrichine S, Rouffineau F, Saulnier L, Giardina T, Bonnin E, Preynat A - J Anim Sci Biotechnol (2015)

Bottom Line: In order to appreciate their in vivo mode of action, the supplementation effect of two of its xylanases, XynD and XynB from families GH10 and GH11 respectively, have been evaluated on two different wheat cultivars Caphorn and Isengrain, which were chosen amongst 6 varieties for their difference in non starch polysaccharides content and arabinoxylan composition.Polysaccharide degradation appeared to occur mainly at the jejunal level and was higher with Isengrain than with Caphorn.For both cultivars, XynD and XynB supplementation increased notably the amount of reducing end sugars into the jejuno-ileal dialysates, which has been confirmed by a valuable increase of the soluble glucose into the jejunal dialysates.

View Article: PubMed Central - PubMed

Affiliation: iSm2 - BiosCiences UMR 7313, Aix Marseille Université, Centrale Marseille, CNRS, Marseille, France.

ABSTRACT

Background: The filamentous fungus Talaromyces versatilis is known to improve the metabolizable energy of wheat-based poultry diets thanks to its ability to produce a pool of CAZymes and particularly endo-β(1,4)-xylanases. In order to appreciate their in vivo mode of action, the supplementation effect of two of its xylanases, XynD and XynB from families GH10 and GH11 respectively, have been evaluated on two different wheat cultivars Caphorn and Isengrain, which were chosen amongst 6 varieties for their difference in non starch polysaccharides content and arabinoxylan composition.

Results: Polysaccharides digestion was followed during 6 h along the digestive tract using the TNO gastrointestinal model-1, to mimic monogastric metabolism. Polysaccharide degradation appeared to occur mainly at the jejunal level and was higher with Isengrain than with Caphorn. For both cultivars, XynD and XynB supplementation increased notably the amount of reducing end sugars into the jejuno-ileal dialysates, which has been confirmed by a valuable increase of the soluble glucose into the jejunal dialysates.

Conclusions: The amounts of arabinose and xylose into the dialysates and ileal deliveries increased consequently mainly for Caphorn, suggesting that XynD and XynB supplementation in wheat-based diet could alleviate the anti-nutritional effects of arabinoxylans by limiting the physical entrapment of starch and could increase the available metabolizable energy.

No MeSH data available.


Cumulative reducing ends in the dialysates with and without enzymatic treatment. The cumulative reducing ends in the jejunal (upper) and ileal (lower) dialysates on Caphorn (left) and Isengrain (right) without xylanase (white and dark) with XynD (full light grey and full dark grey) and with XynB (hatched light grey and hatched dark grey) were expressed as a percentage of the total reducing ends measured after the 360 min of digestion in all the TIM-1 compartments. *Corresponds to the significant differences.
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Fig2: Cumulative reducing ends in the dialysates with and without enzymatic treatment. The cumulative reducing ends in the jejunal (upper) and ileal (lower) dialysates on Caphorn (left) and Isengrain (right) without xylanase (white and dark) with XynD (full light grey and full dark grey) and with XynB (hatched light grey and hatched dark grey) were expressed as a percentage of the total reducing ends measured after the 360 min of digestion in all the TIM-1 compartments. *Corresponds to the significant differences.

Mentions: The total reducing ends were measured, both with and without xylanases added in TIM-1, in order to evaluate the extent of polysaccharide hydrolysis (Figure 2). As the reducing ends reflect both the hydrolysis of starch as well as NSP, these values can be considered as a marker of wheat digestion in the TIM-1. For both wheat cultivars, the total reducing ends in the jejunal and ileal dialysates represented the major part (70-80%) of the reducing ends measured in the TIM-1 after 360 min. Conversely, in the ileal deliveries and gastro-intestinal residues the total reducing ends represented only ~10% in each (data not shown). In accordance with the results presented above, total reducing ends dialyzing at the jejunal and ileal levels in the absence of xylanase (control trial) were in the same range for both cultivars, with 48.4 ± 8.6% and 21.4 ± 2.3% for Caphorn vs 51.7 ± 3.5% and 20.7 ± 0.9% for Isengrain, respectively. The XynD and XynB supplementations increased total reducing ends for both wheat cultivars although the effect was slightly lower on Isengrain. However for Caphorn, XynD was effective all along the digestion tract (+5.3% and +8.1% relative to the control in the jejunal and ileal dialysates, respectively), whereas XynB was mostly effective at the beginning of the digestion (+12.3% and +1.0% vs the control in the jejunal and ileal dialysates, respectively). Conversely, XynD and XynB had a comparable effect on Isengrain with +5.0% and +5.6% in the jejunal dialysate and +2.1% and +2.6% in the ileal dialysate, respectively. Finally, these results suggested that the hydrolysis performed by XynD and XynB in the presence of salivary/pancreatic α(1,4)-amylases occurred mainly in the proximal parts (from the gastric to the jejunal compartments) of the TIM-1.Figure 2


In vitro gastrointestinal digestion study of two wheat cultivars and evaluation of xylanase supplementation.

Lafond M, Bouza B, Eyrichine S, Rouffineau F, Saulnier L, Giardina T, Bonnin E, Preynat A - J Anim Sci Biotechnol (2015)

Cumulative reducing ends in the dialysates with and without enzymatic treatment. The cumulative reducing ends in the jejunal (upper) and ileal (lower) dialysates on Caphorn (left) and Isengrain (right) without xylanase (white and dark) with XynD (full light grey and full dark grey) and with XynB (hatched light grey and hatched dark grey) were expressed as a percentage of the total reducing ends measured after the 360 min of digestion in all the TIM-1 compartments. *Corresponds to the significant differences.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4362821&req=5

Fig2: Cumulative reducing ends in the dialysates with and without enzymatic treatment. The cumulative reducing ends in the jejunal (upper) and ileal (lower) dialysates on Caphorn (left) and Isengrain (right) without xylanase (white and dark) with XynD (full light grey and full dark grey) and with XynB (hatched light grey and hatched dark grey) were expressed as a percentage of the total reducing ends measured after the 360 min of digestion in all the TIM-1 compartments. *Corresponds to the significant differences.
Mentions: The total reducing ends were measured, both with and without xylanases added in TIM-1, in order to evaluate the extent of polysaccharide hydrolysis (Figure 2). As the reducing ends reflect both the hydrolysis of starch as well as NSP, these values can be considered as a marker of wheat digestion in the TIM-1. For both wheat cultivars, the total reducing ends in the jejunal and ileal dialysates represented the major part (70-80%) of the reducing ends measured in the TIM-1 after 360 min. Conversely, in the ileal deliveries and gastro-intestinal residues the total reducing ends represented only ~10% in each (data not shown). In accordance with the results presented above, total reducing ends dialyzing at the jejunal and ileal levels in the absence of xylanase (control trial) were in the same range for both cultivars, with 48.4 ± 8.6% and 21.4 ± 2.3% for Caphorn vs 51.7 ± 3.5% and 20.7 ± 0.9% for Isengrain, respectively. The XynD and XynB supplementations increased total reducing ends for both wheat cultivars although the effect was slightly lower on Isengrain. However for Caphorn, XynD was effective all along the digestion tract (+5.3% and +8.1% relative to the control in the jejunal and ileal dialysates, respectively), whereas XynB was mostly effective at the beginning of the digestion (+12.3% and +1.0% vs the control in the jejunal and ileal dialysates, respectively). Conversely, XynD and XynB had a comparable effect on Isengrain with +5.0% and +5.6% in the jejunal dialysate and +2.1% and +2.6% in the ileal dialysate, respectively. Finally, these results suggested that the hydrolysis performed by XynD and XynB in the presence of salivary/pancreatic α(1,4)-amylases occurred mainly in the proximal parts (from the gastric to the jejunal compartments) of the TIM-1.Figure 2

Bottom Line: In order to appreciate their in vivo mode of action, the supplementation effect of two of its xylanases, XynD and XynB from families GH10 and GH11 respectively, have been evaluated on two different wheat cultivars Caphorn and Isengrain, which were chosen amongst 6 varieties for their difference in non starch polysaccharides content and arabinoxylan composition.Polysaccharide degradation appeared to occur mainly at the jejunal level and was higher with Isengrain than with Caphorn.For both cultivars, XynD and XynB supplementation increased notably the amount of reducing end sugars into the jejuno-ileal dialysates, which has been confirmed by a valuable increase of the soluble glucose into the jejunal dialysates.

View Article: PubMed Central - PubMed

Affiliation: iSm2 - BiosCiences UMR 7313, Aix Marseille Université, Centrale Marseille, CNRS, Marseille, France.

ABSTRACT

Background: The filamentous fungus Talaromyces versatilis is known to improve the metabolizable energy of wheat-based poultry diets thanks to its ability to produce a pool of CAZymes and particularly endo-β(1,4)-xylanases. In order to appreciate their in vivo mode of action, the supplementation effect of two of its xylanases, XynD and XynB from families GH10 and GH11 respectively, have been evaluated on two different wheat cultivars Caphorn and Isengrain, which were chosen amongst 6 varieties for their difference in non starch polysaccharides content and arabinoxylan composition.

Results: Polysaccharides digestion was followed during 6 h along the digestive tract using the TNO gastrointestinal model-1, to mimic monogastric metabolism. Polysaccharide degradation appeared to occur mainly at the jejunal level and was higher with Isengrain than with Caphorn. For both cultivars, XynD and XynB supplementation increased notably the amount of reducing end sugars into the jejuno-ileal dialysates, which has been confirmed by a valuable increase of the soluble glucose into the jejunal dialysates.

Conclusions: The amounts of arabinose and xylose into the dialysates and ileal deliveries increased consequently mainly for Caphorn, suggesting that XynD and XynB supplementation in wheat-based diet could alleviate the anti-nutritional effects of arabinoxylans by limiting the physical entrapment of starch and could increase the available metabolizable energy.

No MeSH data available.