The effects of sarcolipin over-expression in mouse skeletal muscle on metabolic activity.
Bottom Line: The mechanism proposed is uncoupling of the sarcoplasmic reticulum calcium pump.Sarcolipin levels were so low that it is unlikely that knocking out sarcolipin would have a measurable effect on thermogenesis by SERCA.In addition, overexpression of neither wild type nor FLAG-tagged variants of mouse sarcolipin in transgenic mice had any major significant effects on body mass, energy expenditure, even when mice were fed on a high fat diet.
Affiliation: Centre for Biological Sciences, University of Southampton, Southampton SO17 1BJ, UK.Show MeSH
Mentions: The finding that there are no major effects of over-expressing sarcolipin and flag-tagged sarcolipin on body mass as a result of overfeeding is also reflected in the finding that energy expenditure, measured by open circuit indirect calorimetry, was similar for both wild type and transgenic animals (Fig. 5). There were no significant differences, either at 10 weeks of age (before the feeding of the high fat diet) (Fig. 5A) or after 15 weeks on the high fat diet (Fig. 5B), between wild type mice and mice over-expressing sarcolipin or FLAG-tagged sarcolipin in either the diurnal rhythm (main Fig. 5A and B) or in total energy expenditure (inset Fig. 5A and B), whether data were expressed for the whole animal (as in Fig. 5) or relative to body weight (data not shown).
Affiliation: Centre for Biological Sciences, University of Southampton, Southampton SO17 1BJ, UK.