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The effects of sarcolipin over-expression in mouse skeletal muscle on metabolic activity.

Butler J, Smyth N, Broadbridge R, Council CE, Lee AG, Stocker CJ, Hislop DC, Arch JR, Cawthorne MA, Malcolm East J - Arch. Biochem. Biophys. (2015)

Bottom Line: The mechanism proposed is uncoupling of the sarcoplasmic reticulum calcium pump.Sarcolipin levels were so low that it is unlikely that knocking out sarcolipin would have a measurable effect on thermogenesis by SERCA.In addition, overexpression of neither wild type nor FLAG-tagged variants of mouse sarcolipin in transgenic mice had any major significant effects on body mass, energy expenditure, even when mice were fed on a high fat diet.

View Article: PubMed Central - PubMed

Affiliation: Centre for Biological Sciences, University of Southampton, Southampton SO17 1BJ, UK.

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Effect of sarcolipin over-expression and FLAG-sarcolipin expression by skeletal muscle on metabolic rate. Hourly energy expenditure 1 week before (A) and 15 weeks after (B) the introduction of the high fat diet in male wild type (solid line), sarcolipin over-expressing (dashed line) and FLAG-tagged sarcolipin expressing (dotted line). Error bars have been omitted for clarity since the objective is to show that the diurnal rhythm of energy expenditure was similar in all genotypes Total energy expenditure over 24 h at 10 weeks of age and over 22 h at 26 weeks of age is shown in (A inset) and (B inset) respectively; n = 7 cages. There were no significant differences in energy expenditure at any hourly time point or in total.
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f0025: Effect of sarcolipin over-expression and FLAG-sarcolipin expression by skeletal muscle on metabolic rate. Hourly energy expenditure 1 week before (A) and 15 weeks after (B) the introduction of the high fat diet in male wild type (solid line), sarcolipin over-expressing (dashed line) and FLAG-tagged sarcolipin expressing (dotted line). Error bars have been omitted for clarity since the objective is to show that the diurnal rhythm of energy expenditure was similar in all genotypes Total energy expenditure over 24 h at 10 weeks of age and over 22 h at 26 weeks of age is shown in (A inset) and (B inset) respectively; n = 7 cages. There were no significant differences in energy expenditure at any hourly time point or in total.

Mentions: The finding that there are no major effects of over-expressing sarcolipin and flag-tagged sarcolipin on body mass as a result of overfeeding is also reflected in the finding that energy expenditure, measured by open circuit indirect calorimetry, was similar for both wild type and transgenic animals (Fig. 5). There were no significant differences, either at 10 weeks of age (before the feeding of the high fat diet) (Fig. 5A) or after 15 weeks on the high fat diet (Fig. 5B), between wild type mice and mice over-expressing sarcolipin or FLAG-tagged sarcolipin in either the diurnal rhythm (main Fig. 5A and B) or in total energy expenditure (inset Fig. 5A and B), whether data were expressed for the whole animal (as in Fig. 5) or relative to body weight (data not shown).


The effects of sarcolipin over-expression in mouse skeletal muscle on metabolic activity.

Butler J, Smyth N, Broadbridge R, Council CE, Lee AG, Stocker CJ, Hislop DC, Arch JR, Cawthorne MA, Malcolm East J - Arch. Biochem. Biophys. (2015)

Effect of sarcolipin over-expression and FLAG-sarcolipin expression by skeletal muscle on metabolic rate. Hourly energy expenditure 1 week before (A) and 15 weeks after (B) the introduction of the high fat diet in male wild type (solid line), sarcolipin over-expressing (dashed line) and FLAG-tagged sarcolipin expressing (dotted line). Error bars have been omitted for clarity since the objective is to show that the diurnal rhythm of energy expenditure was similar in all genotypes Total energy expenditure over 24 h at 10 weeks of age and over 22 h at 26 weeks of age is shown in (A inset) and (B inset) respectively; n = 7 cages. There were no significant differences in energy expenditure at any hourly time point or in total.
© Copyright Policy - CC BY
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4362768&req=5

f0025: Effect of sarcolipin over-expression and FLAG-sarcolipin expression by skeletal muscle on metabolic rate. Hourly energy expenditure 1 week before (A) and 15 weeks after (B) the introduction of the high fat diet in male wild type (solid line), sarcolipin over-expressing (dashed line) and FLAG-tagged sarcolipin expressing (dotted line). Error bars have been omitted for clarity since the objective is to show that the diurnal rhythm of energy expenditure was similar in all genotypes Total energy expenditure over 24 h at 10 weeks of age and over 22 h at 26 weeks of age is shown in (A inset) and (B inset) respectively; n = 7 cages. There were no significant differences in energy expenditure at any hourly time point or in total.
Mentions: The finding that there are no major effects of over-expressing sarcolipin and flag-tagged sarcolipin on body mass as a result of overfeeding is also reflected in the finding that energy expenditure, measured by open circuit indirect calorimetry, was similar for both wild type and transgenic animals (Fig. 5). There were no significant differences, either at 10 weeks of age (before the feeding of the high fat diet) (Fig. 5A) or after 15 weeks on the high fat diet (Fig. 5B), between wild type mice and mice over-expressing sarcolipin or FLAG-tagged sarcolipin in either the diurnal rhythm (main Fig. 5A and B) or in total energy expenditure (inset Fig. 5A and B), whether data were expressed for the whole animal (as in Fig. 5) or relative to body weight (data not shown).

Bottom Line: The mechanism proposed is uncoupling of the sarcoplasmic reticulum calcium pump.Sarcolipin levels were so low that it is unlikely that knocking out sarcolipin would have a measurable effect on thermogenesis by SERCA.In addition, overexpression of neither wild type nor FLAG-tagged variants of mouse sarcolipin in transgenic mice had any major significant effects on body mass, energy expenditure, even when mice were fed on a high fat diet.

View Article: PubMed Central - PubMed

Affiliation: Centre for Biological Sciences, University of Southampton, Southampton SO17 1BJ, UK.

Show MeSH