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Biogenic silver nanoparticles production and characterization from native stain of Corynebacterium species and its antimicrobial activity

View Article: PubMed Central

ABSTRACT

In the present study, synthesis, characterization, and the antibacterial activity of silver nanoparticles from native isolate of Corynebacterium glutamicum has been reported. Silver nanoparticles were synthesized by challenging the dried biomass of C. glutamicum with aqueous diamine silver ([Ag (NH3)2]+) containing 1 mM AgNO3. Synthesized silver nanoparticles (AgNPs) were characterized by ultraviolet–visible spectroscopy and energy-dispersive X-ray (EDX) spectroscopy analysis. Morphological study of silver nanoparticles was carried out using transmission electron microscopy (TEM) and scanning electron microscope (SEM). The spherical morphology of silver nanoparticles was confirmed from SEM image. The TEM image showed the average particle size of silver nanoparticles was about 15 nm. Silver nanoparticles synthesized from C. glutamicum were found to have enhanced antimicrobial activity against selected pathogenic strains. Silver nanoparticles from pure strains of Corynebacterium species was done by many investigators, but as per the present literature, this is the first report on the production of silver nanoparticles using a native strain of Corynebacterium.

No MeSH data available.


UV–Vis spectra of silver nanoparticles showing maximum absorbance at 450 nm
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Fig2: UV–Vis spectra of silver nanoparticles showing maximum absorbance at 450 nm

Mentions: The silver nanoparticles were characterized by UV–Vis spectroscopy, one of the most widely used techniques for structural characterization of silver nanoparticles (Sun et al. 2001). Biological method of silver nanoparticles synthesis exhibit strong absorption of electromagnetic waves in the visible range due to their optical resonant property, called occurs due to its collective oscillation of conduction electrons, combined with the incident light (Kreibig and Vollmer 1995). Figure 2 shows the UV–Vis absorption spectra of the 96 h old AgNPs sample at 440 nm. The spectra recorded from the C. glutamicum reaction vessel at different reaction times and observed increased intensity in absorption spectra of silver solution with time, indicating the formation of increased number of silver nanoparticles in the solution (the graph is not included); this is similar to the findings of Esumi et al. (2000), Zhang et al. (2005). The prepared aqueous solution of AgNPs showed a strong absorption band between 410 and 440 nm, which is a typical absorption band of spherical Ag nanoparticles due to their surface plasmon.Fig. 2


Biogenic silver nanoparticles production and characterization from native stain of Corynebacterium species and its antimicrobial activity
UV–Vis spectra of silver nanoparticles showing maximum absorbance at 450 nm
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4362738&req=5

Fig2: UV–Vis spectra of silver nanoparticles showing maximum absorbance at 450 nm
Mentions: The silver nanoparticles were characterized by UV–Vis spectroscopy, one of the most widely used techniques for structural characterization of silver nanoparticles (Sun et al. 2001). Biological method of silver nanoparticles synthesis exhibit strong absorption of electromagnetic waves in the visible range due to their optical resonant property, called occurs due to its collective oscillation of conduction electrons, combined with the incident light (Kreibig and Vollmer 1995). Figure 2 shows the UV–Vis absorption spectra of the 96 h old AgNPs sample at 440 nm. The spectra recorded from the C. glutamicum reaction vessel at different reaction times and observed increased intensity in absorption spectra of silver solution with time, indicating the formation of increased number of silver nanoparticles in the solution (the graph is not included); this is similar to the findings of Esumi et al. (2000), Zhang et al. (2005). The prepared aqueous solution of AgNPs showed a strong absorption band between 410 and 440 nm, which is a typical absorption band of spherical Ag nanoparticles due to their surface plasmon.Fig. 2

View Article: PubMed Central

ABSTRACT

In the present study, synthesis, characterization, and the antibacterial activity of silver nanoparticles from native isolate of Corynebacterium glutamicum has been reported. Silver nanoparticles were synthesized by challenging the dried biomass of C. glutamicum with aqueous diamine silver ([Ag (NH3)2]+) containing 1 mM AgNO3. Synthesized silver nanoparticles (AgNPs) were characterized by ultraviolet–visible spectroscopy and energy-dispersive X-ray (EDX) spectroscopy analysis. Morphological study of silver nanoparticles was carried out using transmission electron microscopy (TEM) and scanning electron microscope (SEM). The spherical morphology of silver nanoparticles was confirmed from SEM image. The TEM image showed the average particle size of silver nanoparticles was about 15 nm. Silver nanoparticles synthesized from C. glutamicum were found to have enhanced antimicrobial activity against selected pathogenic strains. Silver nanoparticles from pure strains of Corynebacterium species was done by many investigators, but as per the present literature, this is the first report on the production of silver nanoparticles using a native strain of Corynebacterium.

No MeSH data available.