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Streptokinase production from Streptococcus dysgalactiae subsp. equisimilis SK-6 in the presence of surfactants, growth factors and trace elements

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ABSTRACT

Streptokinase is an extracellular protein secreted by various strains of streptococci and is used clinically as an intravenous thrombolytic agent for the treatment of acute myocardial infarction. It is well established that the fibrinolytic activity of streptokinase originates in its ability to activate plasminogen. The present investigation was carried out to determine the extent of streptokinase production by Streptococcus dysgalactiae subsp. equisimilis SK-6, in the presence of surfactants, growth factors, trace elements and under the influence of different physical parameters. Mineral salt medium was supplemented with different surfactants, growth factors and trace elements. Effects of incubation period and inoculum volume were also studied. Results indicated that the streptokinase yield was higher in the presence of non-ionic surfactants, where Tween 80 supported maximum enzyme production (0.178 U/ml). Growth factors such as glycine and thiamine supplementation resulted in better enzyme production. Trace elements in the form of magnesium sulphate and ferrous sulphate when added in lesser quantity aided higher streptokinase synthesis. Enzyme production was severely affected in the presence of higher concentrations of these inorganic salts. A constant decrease in the enzyme production was observed beyond 48 h of incubation. Among the different inoculum sizes used, 1 % v/v inoculum facilitated highest streptokinase production (0.360 U/ml). The streptokinase production ability of S. dysgalactiae subsp. equisimilis SK-6 offers its potential industrial application for the manufacture of streptokinase.

No MeSH data available.


Effect of FeSO4 supplementation on streptokinase production. Data represent mean ± SD (n = 3); P < 0.05
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Fig4: Effect of FeSO4 supplementation on streptokinase production. Data represent mean ± SD (n = 3); P < 0.05

Mentions: Supplementation of the production medium with FeSO4 enhanced streptokinase production. A gradual increase in streptokinase production was observed between 0.002 and 0.004 % of FeSO4. Highest streptokinase activity (0.392 U/ml) was obtained with 0.004 % (w/v) FeSO4 supplementation to the medium. Beyond this concentration, a steady decrease in streptokinase activity was recorded (Fig. 4).Fig. 4


Streptokinase production from Streptococcus dysgalactiae subsp. equisimilis SK-6 in the presence of surfactants, growth factors and trace elements
Effect of FeSO4 supplementation on streptokinase production. Data represent mean ± SD (n = 3); P < 0.05
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4362735&req=5

Fig4: Effect of FeSO4 supplementation on streptokinase production. Data represent mean ± SD (n = 3); P < 0.05
Mentions: Supplementation of the production medium with FeSO4 enhanced streptokinase production. A gradual increase in streptokinase production was observed between 0.002 and 0.004 % of FeSO4. Highest streptokinase activity (0.392 U/ml) was obtained with 0.004 % (w/v) FeSO4 supplementation to the medium. Beyond this concentration, a steady decrease in streptokinase activity was recorded (Fig. 4).Fig. 4

View Article: PubMed Central - PubMed

ABSTRACT

Streptokinase is an extracellular protein secreted by various strains of streptococci and is used clinically as an intravenous thrombolytic agent for the treatment of acute myocardial infarction. It is well established that the fibrinolytic activity of streptokinase originates in its ability to activate plasminogen. The present investigation was carried out to determine the extent of streptokinase production by Streptococcus dysgalactiae subsp. equisimilis SK-6, in the presence of surfactants, growth factors, trace elements and under the influence of different physical parameters. Mineral salt medium was supplemented with different surfactants, growth factors and trace elements. Effects of incubation period and inoculum volume were also studied. Results indicated that the streptokinase yield was higher in the presence of non-ionic surfactants, where Tween 80 supported maximum enzyme production (0.178&nbsp;U/ml). Growth factors such as glycine and thiamine supplementation resulted in better enzyme production. Trace elements in the form of magnesium sulphate and ferrous sulphate when added in lesser quantity aided higher streptokinase synthesis. Enzyme production was severely affected in the presence of higher concentrations of these inorganic salts. A constant decrease in the enzyme production was observed beyond 48&nbsp;h of incubation. Among the different inoculum sizes used, 1&nbsp;% v/v inoculum facilitated highest streptokinase production (0.360&nbsp;U/ml). The streptokinase production ability of S. dysgalactiae subsp. equisimilis SK-6 offers its potential industrial application for the manufacture of streptokinase.

No MeSH data available.