Injury-stimulated Hedgehog signaling promotes regenerative proliferation of Drosophila intestinal stem cells.
Bottom Line: Many adult tissues are maintained by resident stem cells that elevate their proliferation in response to injury.Here we show that injury induces Hedgehog (Hh) signaling in enteroblasts (EBs) to promote intestinal stem cell (ISC) proliferation in Drosophila melanogaster adult midgut.Hence, our study uncovers a JNK-Hh-JAK-STAT signaling axis in the regulation of regenerative stem cell proliferation.
Affiliation: Department of Developmental Biology and Department of Pharmacology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390.Show MeSH
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Mentions: The observation that Hh signaling is not essential for basal ISC proliferation but is required for damage-induced ISC proliferation prompted us to examine whether Hh pathway activity is up-regulated in response to injury. Using RT-qPCR, we found that the expression of the Hh pathway target gene ptc as well as hh itself was up-regulated in guts treated with DSS; however, expression of hh was not induced by bleomycin treatment (Fig. 5 A and Fig. S2).
Affiliation: Department of Developmental Biology and Department of Pharmacology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390.