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PAPC mediates self/non-self-distinction during Snail1-dependent tissue separation.

Luu O, Damm EW, Parent SE, Barua D, Smith TH, Wen JW, Lepage SE, Nagel M, Ibrahim-Gawel H, Huang Y, Bruce AE, Winklbauer R - J. Cell Biol. (2015)

Bottom Line: First, PAPC attenuates planar cell polarity signaling at the ectoderm-mesoderm boundary to lower cell adhesion and facilitate cleft formation.It consists of short stretches of adherens junction-like contacts inserted between intermediate-sized contacts and large intercellular gaps.These roles of PAPC constitute a self/non-self-recognition mechanism that determines the site of boundary formation at the interface between PAPC-expressing and -nonexpressing cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Cell and Systems Biology, University of Toronto, Toronto, Ontario, Canada M5S 3G5.

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Ultrastructure of Brachet’s cleft. (A) Low-magnification view of Brachet’s cleft. ECM stained with LN. Note size differences of yolk platelets (y) in mesoderm versus ectoderm. (B–E) Contacts between ectoderm and mesoderm cell in absence of ECM staining. Boxes in B indicate regions shown at higher magnification in D–E. e, ectodermal BCR; m, prechordal mesoderm; l, large gaps; i, intermediate, gap-like contacts; c, close, adherens junction–like contacts. Arrows in D indicate point of divergence of membranes at end of intermediate contact and in E indicate characteristics of adherens junctions at high cadherin density.
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fig8: Ultrastructure of Brachet’s cleft. (A) Low-magnification view of Brachet’s cleft. ECM stained with LN. Note size differences of yolk platelets (y) in mesoderm versus ectoderm. (B–E) Contacts between ectoderm and mesoderm cell in absence of ECM staining. Boxes in B indicate regions shown at higher magnification in D–E. e, ectodermal BCR; m, prechordal mesoderm; l, large gaps; i, intermediate, gap-like contacts; c, close, adherens junction–like contacts. Arrows in D indicate point of divergence of membranes at end of intermediate contact and in E indicate characteristics of adherens junctions at high cadherin density.

Mentions: To analyze cell–cell interactions across ectoderm–mesoderm boundaries at high resolution, we performed transmission EM (TEM). At low magnification, Brachet’s cleft appeared as an ECM-filled space of irregular width (Fig. 8 A). Large micrometer-sized gaps were intermixed with narrower contacts (Fig. 8 B), which in turn consisted of intermediate contacts 100–300 nm wide and adherens junction–like close contacts <30 nm wide (Fig. 8, C–E), and compatible with cadherin adhesion (Farquhar and Palade, 1963; Miyaguchi, 2000; Tepass et al., 2000). Close contacts occupied 17 ± 1.5% of the cleft length (n = 12 embryos). At intermediate contacts, membranes of adjacent cells typically ran in parallel, bending sharply when they opened into larger spaces, suggesting that cell adhesion occurred across these contacts.


PAPC mediates self/non-self-distinction during Snail1-dependent tissue separation.

Luu O, Damm EW, Parent SE, Barua D, Smith TH, Wen JW, Lepage SE, Nagel M, Ibrahim-Gawel H, Huang Y, Bruce AE, Winklbauer R - J. Cell Biol. (2015)

Ultrastructure of Brachet’s cleft. (A) Low-magnification view of Brachet’s cleft. ECM stained with LN. Note size differences of yolk platelets (y) in mesoderm versus ectoderm. (B–E) Contacts between ectoderm and mesoderm cell in absence of ECM staining. Boxes in B indicate regions shown at higher magnification in D–E. e, ectodermal BCR; m, prechordal mesoderm; l, large gaps; i, intermediate, gap-like contacts; c, close, adherens junction–like contacts. Arrows in D indicate point of divergence of membranes at end of intermediate contact and in E indicate characteristics of adherens junctions at high cadherin density.
© Copyright Policy - openaccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4362454&req=5

fig8: Ultrastructure of Brachet’s cleft. (A) Low-magnification view of Brachet’s cleft. ECM stained with LN. Note size differences of yolk platelets (y) in mesoderm versus ectoderm. (B–E) Contacts between ectoderm and mesoderm cell in absence of ECM staining. Boxes in B indicate regions shown at higher magnification in D–E. e, ectodermal BCR; m, prechordal mesoderm; l, large gaps; i, intermediate, gap-like contacts; c, close, adherens junction–like contacts. Arrows in D indicate point of divergence of membranes at end of intermediate contact and in E indicate characteristics of adherens junctions at high cadherin density.
Mentions: To analyze cell–cell interactions across ectoderm–mesoderm boundaries at high resolution, we performed transmission EM (TEM). At low magnification, Brachet’s cleft appeared as an ECM-filled space of irregular width (Fig. 8 A). Large micrometer-sized gaps were intermixed with narrower contacts (Fig. 8 B), which in turn consisted of intermediate contacts 100–300 nm wide and adherens junction–like close contacts <30 nm wide (Fig. 8, C–E), and compatible with cadherin adhesion (Farquhar and Palade, 1963; Miyaguchi, 2000; Tepass et al., 2000). Close contacts occupied 17 ± 1.5% of the cleft length (n = 12 embryos). At intermediate contacts, membranes of adjacent cells typically ran in parallel, bending sharply when they opened into larger spaces, suggesting that cell adhesion occurred across these contacts.

Bottom Line: First, PAPC attenuates planar cell polarity signaling at the ectoderm-mesoderm boundary to lower cell adhesion and facilitate cleft formation.It consists of short stretches of adherens junction-like contacts inserted between intermediate-sized contacts and large intercellular gaps.These roles of PAPC constitute a self/non-self-recognition mechanism that determines the site of boundary formation at the interface between PAPC-expressing and -nonexpressing cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Cell and Systems Biology, University of Toronto, Toronto, Ontario, Canada M5S 3G5.

Show MeSH
Related in: MedlinePlus