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PAPC mediates self/non-self-distinction during Snail1-dependent tissue separation.

Luu O, Damm EW, Parent SE, Barua D, Smith TH, Wen JW, Lepage SE, Nagel M, Ibrahim-Gawel H, Huang Y, Bruce AE, Winklbauer R - J. Cell Biol. (2015)

Bottom Line: First, PAPC attenuates planar cell polarity signaling at the ectoderm-mesoderm boundary to lower cell adhesion and facilitate cleft formation.It consists of short stretches of adherens junction-like contacts inserted between intermediate-sized contacts and large intercellular gaps.These roles of PAPC constitute a self/non-self-recognition mechanism that determines the site of boundary formation at the interface between PAPC-expressing and -nonexpressing cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Cell and Systems Biology, University of Toronto, Toronto, Ontario, Canada M5S 3G5.

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Cleft contact formation. (A) Reaggregation of normal (FDA labeled) and M-PAPC–expressing (RDA) BCR cells. Arrowheads indicate example of cleft contact; blue and yellow dots and arrows indicate gliding movement; cell groups move apart in the direction of the red arrows. (B) Reaggregating prechordal mesoderm (RDA) and BCR (FDA) cells. Gliding of cells marked as in A. (C) Frequency of cleft contacts after 30 min of reaggregation. 95% confidence intervals indicated; n, number of cell pairs. (D) Expansion of initial cleft contact (arrows) between reaggregating ectoderm and mesoderm cells. (E) Exemplary kymographs of cell contacts in reaggregated cells quantitated in C. Black arrows indicate the position of cell–cell contact. White or gray, close contact; dark, cleft contact (pointed out by white arrows when only occasionally present).
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fig4: Cleft contact formation. (A) Reaggregation of normal (FDA labeled) and M-PAPC–expressing (RDA) BCR cells. Arrowheads indicate example of cleft contact; blue and yellow dots and arrows indicate gliding movement; cell groups move apart in the direction of the red arrows. (B) Reaggregating prechordal mesoderm (RDA) and BCR (FDA) cells. Gliding of cells marked as in A. (C) Frequency of cleft contacts after 30 min of reaggregation. 95% confidence intervals indicated; n, number of cell pairs. (D) Expansion of initial cleft contact (arrows) between reaggregating ectoderm and mesoderm cells. (E) Exemplary kymographs of cell contacts in reaggregated cells quantitated in C. Black arrows indicate the position of cell–cell contact. White or gray, close contact; dark, cleft contact (pointed out by white arrows when only occasionally present).

Mentions: To examine this interaction, we reaggregated mixtures of M-PAPC+ and M-PAPC− BCR cells (Fig. 4 A; Fig. S4, A and B; and Videos 1 and 2). Cells of the same type attached rapidly. M-PAPC+ and M-PAPC− cells also formed stable contacts, but appeared to remain separated by a narrow gap when cells were visualized by injected fluorescent dextran (Fig. 4, A, C, and E; and Fig. S4 B). Although these heterotypic contacts resisted pulling, cells slipped easily past each other at these gap-like interfaces (Fig. 4 A and Videos 1 and 2). Thus, although tight contacts between cells of the same type appeared similar (Fig. 4, A, C, and E), contacts between different cell types appeared cleft-like, yet adhesive and permitted rapid lateral exchange of cell neighbors. Herein, we refer to these adhesive structures as cleft contacts.


PAPC mediates self/non-self-distinction during Snail1-dependent tissue separation.

Luu O, Damm EW, Parent SE, Barua D, Smith TH, Wen JW, Lepage SE, Nagel M, Ibrahim-Gawel H, Huang Y, Bruce AE, Winklbauer R - J. Cell Biol. (2015)

Cleft contact formation. (A) Reaggregation of normal (FDA labeled) and M-PAPC–expressing (RDA) BCR cells. Arrowheads indicate example of cleft contact; blue and yellow dots and arrows indicate gliding movement; cell groups move apart in the direction of the red arrows. (B) Reaggregating prechordal mesoderm (RDA) and BCR (FDA) cells. Gliding of cells marked as in A. (C) Frequency of cleft contacts after 30 min of reaggregation. 95% confidence intervals indicated; n, number of cell pairs. (D) Expansion of initial cleft contact (arrows) between reaggregating ectoderm and mesoderm cells. (E) Exemplary kymographs of cell contacts in reaggregated cells quantitated in C. Black arrows indicate the position of cell–cell contact. White or gray, close contact; dark, cleft contact (pointed out by white arrows when only occasionally present).
© Copyright Policy - openaccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4362454&req=5

fig4: Cleft contact formation. (A) Reaggregation of normal (FDA labeled) and M-PAPC–expressing (RDA) BCR cells. Arrowheads indicate example of cleft contact; blue and yellow dots and arrows indicate gliding movement; cell groups move apart in the direction of the red arrows. (B) Reaggregating prechordal mesoderm (RDA) and BCR (FDA) cells. Gliding of cells marked as in A. (C) Frequency of cleft contacts after 30 min of reaggregation. 95% confidence intervals indicated; n, number of cell pairs. (D) Expansion of initial cleft contact (arrows) between reaggregating ectoderm and mesoderm cells. (E) Exemplary kymographs of cell contacts in reaggregated cells quantitated in C. Black arrows indicate the position of cell–cell contact. White or gray, close contact; dark, cleft contact (pointed out by white arrows when only occasionally present).
Mentions: To examine this interaction, we reaggregated mixtures of M-PAPC+ and M-PAPC− BCR cells (Fig. 4 A; Fig. S4, A and B; and Videos 1 and 2). Cells of the same type attached rapidly. M-PAPC+ and M-PAPC− cells also formed stable contacts, but appeared to remain separated by a narrow gap when cells were visualized by injected fluorescent dextran (Fig. 4, A, C, and E; and Fig. S4 B). Although these heterotypic contacts resisted pulling, cells slipped easily past each other at these gap-like interfaces (Fig. 4 A and Videos 1 and 2). Thus, although tight contacts between cells of the same type appeared similar (Fig. 4, A, C, and E), contacts between different cell types appeared cleft-like, yet adhesive and permitted rapid lateral exchange of cell neighbors. Herein, we refer to these adhesive structures as cleft contacts.

Bottom Line: First, PAPC attenuates planar cell polarity signaling at the ectoderm-mesoderm boundary to lower cell adhesion and facilitate cleft formation.It consists of short stretches of adherens junction-like contacts inserted between intermediate-sized contacts and large intercellular gaps.These roles of PAPC constitute a self/non-self-recognition mechanism that determines the site of boundary formation at the interface between PAPC-expressing and -nonexpressing cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Cell and Systems Biology, University of Toronto, Toronto, Ontario, Canada M5S 3G5.

Show MeSH
Related in: MedlinePlus