PAPC mediates self/non-self-distinction during Snail1-dependent tissue separation.
Bottom Line: First, PAPC attenuates planar cell polarity signaling at the ectoderm-mesoderm boundary to lower cell adhesion and facilitate cleft formation.It consists of short stretches of adherens junction-like contacts inserted between intermediate-sized contacts and large intercellular gaps.These roles of PAPC constitute a self/non-self-recognition mechanism that determines the site of boundary formation at the interface between PAPC-expressing and -nonexpressing cells.
Affiliation: Department of Cell and Systems Biology, University of Toronto, Toronto, Ontario, Canada M5S 3G5.Show MeSH
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Mentions: To examine this interaction, we reaggregated mixtures of M-PAPC+ and M-PAPC− BCR cells (Fig. 4 A; Fig. S4, A and B; and Videos 1 and 2). Cells of the same type attached rapidly. M-PAPC+ and M-PAPC− cells also formed stable contacts, but appeared to remain separated by a narrow gap when cells were visualized by injected fluorescent dextran (Fig. 4, A, C, and E; and Fig. S4 B). Although these heterotypic contacts resisted pulling, cells slipped easily past each other at these gap-like interfaces (Fig. 4 A and Videos 1 and 2). Thus, although tight contacts between cells of the same type appeared similar (Fig. 4, A, C, and E), contacts between different cell types appeared cleft-like, yet adhesive and permitted rapid lateral exchange of cell neighbors. Herein, we refer to these adhesive structures as cleft contacts.
Affiliation: Department of Cell and Systems Biology, University of Toronto, Toronto, Ontario, Canada M5S 3G5.