PAPC mediates self/non-self-distinction during Snail1-dependent tissue separation.
Bottom Line: First, PAPC attenuates planar cell polarity signaling at the ectoderm-mesoderm boundary to lower cell adhesion and facilitate cleft formation.It consists of short stretches of adherens junction-like contacts inserted between intermediate-sized contacts and large intercellular gaps.These roles of PAPC constitute a self/non-self-recognition mechanism that determines the site of boundary formation at the interface between PAPC-expressing and -nonexpressing cells.
Affiliation: Department of Cell and Systems Biology, University of Toronto, Toronto, Ontario, Canada M5S 3G5.Show MeSH
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Mentions: Xsnail1 is expressed in the mesoderm of Xenopus gastrulae (Essex et al., 1993). To identify its function, morpholino antisense oligonucleotides (MOs) were injected into dorsal blastomeres. In uninjected or 5-mismatch control MO (5mis-MO)â€“injected embryos, Brachetâ€™s cleft separated the prechordal mesoderm from the ectodermal BCR (Fig. 1 A). Injection of Snail1-MO eliminated cleft formation in this region (Fig. 1 B), and coinjection of Xsnail1 mRNA rescued it (Fig. 1 C), suggesting that Xsnail1 is required for tissue separation. Cleft defects in Xsnail1 morphants were not accompanied by any noticeable changes in mesoderm specification (Fig. S1, Aâ€“D).
Affiliation: Department of Cell and Systems Biology, University of Toronto, Toronto, Ontario, Canada M5S 3G5.