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Microengineering in cardiovascular research: new developments and translational applications.

Chan JM, Wong KH, Richards AM, Drum CL - Cardiovasc. Res. (2015)

Bottom Line: Microfluidic, cellular co-cultures that approximate macro-scale biology are important tools for refining the in vitro study of organ-level function and disease.Here we review applications of these technologies specific to the cardiovascular field, emphasizing three general categories of use: reductionist vascular models, tissue-engineered vascular models, and point-of-care diagnostics.With continued progress in the ability to purposefully control microscale environments, the detailed study of both primary and cultured cells may find new relevance in the general cardiovascular research community.

View Article: PubMed Central - PubMed

Affiliation: School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore.

No MeSH data available.


Microfluidic point-of-care devices. (A) Schematic representation of hsCRP capture chip. (B) Photograph of chip with annotations to the various chambers and channels. Reproduced with permission from Lee et al.51 (C) Illustration of CM enrichment device with an array of 30 µm posts. CM purity increases from outlet #1 to #7. (D) Photograph of the CM enrichment device with colour dyes illustrating stable hydrodynamic focusing of the blue dye by flanking red dyes into the centre of the chamber. A filtering device upstream of the sorting chamber eliminates cell clumps. Reproduced with permission from Martinez et al.52
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CVV049F5: Microfluidic point-of-care devices. (A) Schematic representation of hsCRP capture chip. (B) Photograph of chip with annotations to the various chambers and channels. Reproduced with permission from Lee et al.51 (C) Illustration of CM enrichment device with an array of 30 µm posts. CM purity increases from outlet #1 to #7. (D) Photograph of the CM enrichment device with colour dyes illustrating stable hydrodynamic focusing of the blue dye by flanking red dyes into the centre of the chamber. A filtering device upstream of the sorting chamber eliminates cell clumps. Reproduced with permission from Martinez et al.52

Mentions: In another use of surface-coated POCT electrochemical immunoassay, researchers used microfluidic devices to capture hsCRP, a classic acute phase plasma protein that increases rapidly in cardiovascular disease, stroke, tissue infection, or inflammation.51 The researchers designed a microfluidic-based hsCRP test using ELISA and experimentally measured electrical current as a function of the concentration of the alkaline phosphatase (ALP)-labelled CRP antigen–antibody complex in the microfluidic chip (Figure 5A and B). The LOD of the chip was 0.1 mg/L CRP in serum samples, which is the concentration of hsCRP in a physiologically relevant range.Figure 5


Microengineering in cardiovascular research: new developments and translational applications.

Chan JM, Wong KH, Richards AM, Drum CL - Cardiovasc. Res. (2015)

Microfluidic point-of-care devices. (A) Schematic representation of hsCRP capture chip. (B) Photograph of chip with annotations to the various chambers and channels. Reproduced with permission from Lee et al.51 (C) Illustration of CM enrichment device with an array of 30 µm posts. CM purity increases from outlet #1 to #7. (D) Photograph of the CM enrichment device with colour dyes illustrating stable hydrodynamic focusing of the blue dye by flanking red dyes into the centre of the chamber. A filtering device upstream of the sorting chamber eliminates cell clumps. Reproduced with permission from Martinez et al.52
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4362405&req=5

CVV049F5: Microfluidic point-of-care devices. (A) Schematic representation of hsCRP capture chip. (B) Photograph of chip with annotations to the various chambers and channels. Reproduced with permission from Lee et al.51 (C) Illustration of CM enrichment device with an array of 30 µm posts. CM purity increases from outlet #1 to #7. (D) Photograph of the CM enrichment device with colour dyes illustrating stable hydrodynamic focusing of the blue dye by flanking red dyes into the centre of the chamber. A filtering device upstream of the sorting chamber eliminates cell clumps. Reproduced with permission from Martinez et al.52
Mentions: In another use of surface-coated POCT electrochemical immunoassay, researchers used microfluidic devices to capture hsCRP, a classic acute phase plasma protein that increases rapidly in cardiovascular disease, stroke, tissue infection, or inflammation.51 The researchers designed a microfluidic-based hsCRP test using ELISA and experimentally measured electrical current as a function of the concentration of the alkaline phosphatase (ALP)-labelled CRP antigen–antibody complex in the microfluidic chip (Figure 5A and B). The LOD of the chip was 0.1 mg/L CRP in serum samples, which is the concentration of hsCRP in a physiologically relevant range.Figure 5

Bottom Line: Microfluidic, cellular co-cultures that approximate macro-scale biology are important tools for refining the in vitro study of organ-level function and disease.Here we review applications of these technologies specific to the cardiovascular field, emphasizing three general categories of use: reductionist vascular models, tissue-engineered vascular models, and point-of-care diagnostics.With continued progress in the ability to purposefully control microscale environments, the detailed study of both primary and cultured cells may find new relevance in the general cardiovascular research community.

View Article: PubMed Central - PubMed

Affiliation: School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore.

No MeSH data available.