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Isolation and identification of indigenous plant growth promoting rhizobacteria from Himalayan region of Kashmir and their effect on improving growth and nutrient contents of maize (Zea mays L.).

Zahid M, Abbasi MK, Hameed S, Rahim N - Front Microbiol (2015)

Bottom Line: Isolates were then further tested for their effects on growth and nutrient contents of maize (Z. mays L.) under pouch and pot conditions.The isolates HJR1, HJR2, HJR3, and HJR5 showed positive activity in acetylene reduction assay showing their N2-fixation potential.Among the eight isolates, HR3 showed a marked P-solubilizing activity, plant growth-promoting attributes, and the potential to be developed as a biofertilizers for integrated nutrient management strategies.

View Article: PubMed Central - PubMed

Affiliation: Department of Soil and Environmental Sciences, Faculty of Agriculture, The University of Poonch Rawalakot, Azad Jammu and Kashmir, Pakistan.

ABSTRACT
Introduction and exploitation of plant growth promoting rhizobacteria (PGPR) in agro-ecosystems enhance plant-microbes interactions that may affect ecosystems sustainability, agricultural productivity, and environmental quality. The present study was conducted to isolate and identify PGPRs associated with maize (Zea mays L.) from twenty sites of Himalayan region of Hajira-Rawalakot, Azad Jammu and Kashmir (AJK), Pakistan. A total of 100 isolates were isolated from these sites, out of which eight (HJR1, HJR2, HJR3, HJR4, HJR5, MR6, HJR7, HJR8) were selected in vitro for their plant growth promoting ability (PGPA) including phosphorus solubilization, indole-3-acetic acid (IAA) production and N2 fixation. The 16S rRNA gene sequencing technique was used for molecular identity and authentication. Isolates were then further tested for their effects on growth and nutrient contents of maize (Z. mays L.) under pouch and pot conditions. The 16S rRNA gene sequencing and phylogenetic analysis identified these isolates belong to Pseudomonas and Bacillus genera. The isolates promoted plant growth by solubilizing soil P which ranged between 19.2 and 35.6 μg mL(-1). The isolates HJR1, HJR2, HJR3, and HJR5 showed positive activity in acetylene reduction assay showing their N2-fixation potential. All eight isolates showed the potential to produce IAA in the range of 0.9-5.39 μg mL(-1) and promote plant growth. Results from a subsequent pot experiment indicated PGPRs distinctly increased maize shoot and root length, shoot and root dry weight, root surface area, leaf surface area, shoot and root N and P contents. Among the eight isolates, HR3 showed a marked P-solubilizing activity, plant growth-promoting attributes, and the potential to be developed as a biofertilizers for integrated nutrient management strategies.

No MeSH data available.


Phylogenetic tree of 16S rRNA gene sequences showing the relationships among the isolates isolated from the soils of Himalayan region of Hajira Rawalakot, Azad Jammu and Kashmir (AJK), Pakistan and the related genera. The data of type strains of related species were from GenBank database (the accession numbers are given in parentheses).
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Figure 1: Phylogenetic tree of 16S rRNA gene sequences showing the relationships among the isolates isolated from the soils of Himalayan region of Hajira Rawalakot, Azad Jammu and Kashmir (AJK), Pakistan and the related genera. The data of type strains of related species were from GenBank database (the accession numbers are given in parentheses).

Mentions: The sequence analysis of 1.5 kb fragment of 16S rRNA gene of all eight bacterial isolates was analyzed by nucleotide Blast analysis. The sequence of isolates HJR1 and HJR3 showed 99% similarity with Bacillus subtilis and were submitted to GenBank under accession number HQ700330 and HQ700332, respectively. Isolates HJR2, HJR4, and HJR8 had 99% sequence match with that of Bacillus megaterium. These isolates were submitted to GenBank under accession number HQ700331, HQ700333, and HQ700334, respectively. Isolate MR6 showed similarity with genus Pseudomonas and was identified as Pseudomonas stutzeri. These sequences were aligned with sequence of some PGPR of different genera and species within genera from the GenBank database. The phylogenetic tree of these strains constructed by using their 16S rRNA sequences (Figure 1) showed that the selected isolates were members of genus Pseudomonas and Bacillus.


Isolation and identification of indigenous plant growth promoting rhizobacteria from Himalayan region of Kashmir and their effect on improving growth and nutrient contents of maize (Zea mays L.).

Zahid M, Abbasi MK, Hameed S, Rahim N - Front Microbiol (2015)

Phylogenetic tree of 16S rRNA gene sequences showing the relationships among the isolates isolated from the soils of Himalayan region of Hajira Rawalakot, Azad Jammu and Kashmir (AJK), Pakistan and the related genera. The data of type strains of related species were from GenBank database (the accession numbers are given in parentheses).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4362393&req=5

Figure 1: Phylogenetic tree of 16S rRNA gene sequences showing the relationships among the isolates isolated from the soils of Himalayan region of Hajira Rawalakot, Azad Jammu and Kashmir (AJK), Pakistan and the related genera. The data of type strains of related species were from GenBank database (the accession numbers are given in parentheses).
Mentions: The sequence analysis of 1.5 kb fragment of 16S rRNA gene of all eight bacterial isolates was analyzed by nucleotide Blast analysis. The sequence of isolates HJR1 and HJR3 showed 99% similarity with Bacillus subtilis and were submitted to GenBank under accession number HQ700330 and HQ700332, respectively. Isolates HJR2, HJR4, and HJR8 had 99% sequence match with that of Bacillus megaterium. These isolates were submitted to GenBank under accession number HQ700331, HQ700333, and HQ700334, respectively. Isolate MR6 showed similarity with genus Pseudomonas and was identified as Pseudomonas stutzeri. These sequences were aligned with sequence of some PGPR of different genera and species within genera from the GenBank database. The phylogenetic tree of these strains constructed by using their 16S rRNA sequences (Figure 1) showed that the selected isolates were members of genus Pseudomonas and Bacillus.

Bottom Line: Isolates were then further tested for their effects on growth and nutrient contents of maize (Z. mays L.) under pouch and pot conditions.The isolates HJR1, HJR2, HJR3, and HJR5 showed positive activity in acetylene reduction assay showing their N2-fixation potential.Among the eight isolates, HR3 showed a marked P-solubilizing activity, plant growth-promoting attributes, and the potential to be developed as a biofertilizers for integrated nutrient management strategies.

View Article: PubMed Central - PubMed

Affiliation: Department of Soil and Environmental Sciences, Faculty of Agriculture, The University of Poonch Rawalakot, Azad Jammu and Kashmir, Pakistan.

ABSTRACT
Introduction and exploitation of plant growth promoting rhizobacteria (PGPR) in agro-ecosystems enhance plant-microbes interactions that may affect ecosystems sustainability, agricultural productivity, and environmental quality. The present study was conducted to isolate and identify PGPRs associated with maize (Zea mays L.) from twenty sites of Himalayan region of Hajira-Rawalakot, Azad Jammu and Kashmir (AJK), Pakistan. A total of 100 isolates were isolated from these sites, out of which eight (HJR1, HJR2, HJR3, HJR4, HJR5, MR6, HJR7, HJR8) were selected in vitro for their plant growth promoting ability (PGPA) including phosphorus solubilization, indole-3-acetic acid (IAA) production and N2 fixation. The 16S rRNA gene sequencing technique was used for molecular identity and authentication. Isolates were then further tested for their effects on growth and nutrient contents of maize (Z. mays L.) under pouch and pot conditions. The 16S rRNA gene sequencing and phylogenetic analysis identified these isolates belong to Pseudomonas and Bacillus genera. The isolates promoted plant growth by solubilizing soil P which ranged between 19.2 and 35.6 μg mL(-1). The isolates HJR1, HJR2, HJR3, and HJR5 showed positive activity in acetylene reduction assay showing their N2-fixation potential. All eight isolates showed the potential to produce IAA in the range of 0.9-5.39 μg mL(-1) and promote plant growth. Results from a subsequent pot experiment indicated PGPRs distinctly increased maize shoot and root length, shoot and root dry weight, root surface area, leaf surface area, shoot and root N and P contents. Among the eight isolates, HR3 showed a marked P-solubilizing activity, plant growth-promoting attributes, and the potential to be developed as a biofertilizers for integrated nutrient management strategies.

No MeSH data available.