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Transcriptome analyses to investigate symbiotic relationships between marine protists.

Balzano S, Corre E, Decelle J, Sierra R, Wincker P, Da Silva C, Poulain J, Pawlowski J, Not F - Front Microbiol (2015)

Bottom Line: Unigenes coding putative c-type lectin domains (CTLD) were found in the species bearing photosynthetic symbionts (A. elongata, Collozoum sp., and S. streptacantha) but not in the non-symbiotic one (A. scolymantha).More particularly, phylogenetic analyses group CTLDs from A. elongata and Collozoum sp. on a distinct branch from S. streptacantha CTLDs, which contained carbohydrate-binding motifs typically observed in other marine photosymbiosis.Our data suggest that similarly to other well-known marine photosymbiosis involving metazoans, the interactions of glycans with c-type lectins is likely involved in modulation of the host/symbiont specific recognition in Radiolaria.

View Article: PubMed Central - PubMed

Affiliation: UMR 7144, Université Pierre et Marie Curie Université Paris 06, Sorbonne Universités, Station Biologique de Roscoff Roscoff, France ; Centre National de la Recherche Scientifique, UMR 7144, Station Biologique de Roscoff Roscoff, France.

ABSTRACT
Rhizaria are an important component of oceanic plankton communities worldwide. A number of species harbor eukaryotic microalgal symbionts, which are horizontally acquired in the environment at each generation. Although these photosymbioses are determinant for Rhizaria ability to thrive in oceanic ecosystems, the mechanisms for symbiotic interactions are unclear. Using high-throughput sequencing technology (i.e., 454), we generated large Expressed Sequence Tag (EST) datasets from four uncultured Rhizaria, an acantharian (Amphilonche elongata), two polycystines (Collozoum sp. and Spongosphaera streptacantha), and one phaeodarian (Aulacantha scolymantha). We assessed the main genetic features of the host/symbionts consortium (i.e., the holobiont) transcriptomes and found rRNA sequences affiliated to a wide range of bacteria and protists in all samples, suggesting that diverse microbial communities are associated with the holobionts. A particular focus was then carried out to search for genes potentially involved in symbiotic processes such as the presence of c-type lectins-coding genes, which are proteins that play a role in cell recognition among eukaryotes. Unigenes coding putative c-type lectin domains (CTLD) were found in the species bearing photosynthetic symbionts (A. elongata, Collozoum sp., and S. streptacantha) but not in the non-symbiotic one (A. scolymantha). More particularly, phylogenetic analyses group CTLDs from A. elongata and Collozoum sp. on a distinct branch from S. streptacantha CTLDs, which contained carbohydrate-binding motifs typically observed in other marine photosymbiosis. Our data suggest that similarly to other well-known marine photosymbiosis involving metazoans, the interactions of glycans with c-type lectins is likely involved in modulation of the host/symbiont specific recognition in Radiolaria.

No MeSH data available.


Venn diagram for pooled sequence clustering based on cd-hit. A total of 124,979 clusters were obtained using 50% similarity. Values indicate the number of clusters relative to each diagram section. Percentages show portions of clusters which only occur in one specimen.
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Figure 4: Venn diagram for pooled sequence clustering based on cd-hit. A total of 124,979 clusters were obtained using 50% similarity. Values indicate the number of clusters relative to each diagram section. Percentages show portions of clusters which only occur in one specimen.

Mentions: The majority of the clusters identified here, based on a 50% similarity, only include sequences from one sample (Figure 4). For example 83% of the clusters containing sequences from A. elongata do not contain unigenes from other samples (Figure 4), and this percentage rises up to 94% for A. scolymantha. Very few clusters thus contain sequences from multiple samples. In spite of the taxonomic relatedness of our specimens only 130 clusters include unigenes from all our samples whereas higher proportions of the clusters are shared between A. elongata and Collozoum sp. (899), Collozoum sp. and S. streptacantha (1170), and A. elongata and S. streptacantha (951) compared to the clusters shared between A. scolymantha and any of the three other holobionts (Figure 4). These differences might be related to the lack of photosymbionts within A. scolymantha, in contrast with the other samples.


Transcriptome analyses to investigate symbiotic relationships between marine protists.

Balzano S, Corre E, Decelle J, Sierra R, Wincker P, Da Silva C, Poulain J, Pawlowski J, Not F - Front Microbiol (2015)

Venn diagram for pooled sequence clustering based on cd-hit. A total of 124,979 clusters were obtained using 50% similarity. Values indicate the number of clusters relative to each diagram section. Percentages show portions of clusters which only occur in one specimen.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4362344&req=5

Figure 4: Venn diagram for pooled sequence clustering based on cd-hit. A total of 124,979 clusters were obtained using 50% similarity. Values indicate the number of clusters relative to each diagram section. Percentages show portions of clusters which only occur in one specimen.
Mentions: The majority of the clusters identified here, based on a 50% similarity, only include sequences from one sample (Figure 4). For example 83% of the clusters containing sequences from A. elongata do not contain unigenes from other samples (Figure 4), and this percentage rises up to 94% for A. scolymantha. Very few clusters thus contain sequences from multiple samples. In spite of the taxonomic relatedness of our specimens only 130 clusters include unigenes from all our samples whereas higher proportions of the clusters are shared between A. elongata and Collozoum sp. (899), Collozoum sp. and S. streptacantha (1170), and A. elongata and S. streptacantha (951) compared to the clusters shared between A. scolymantha and any of the three other holobionts (Figure 4). These differences might be related to the lack of photosymbionts within A. scolymantha, in contrast with the other samples.

Bottom Line: Unigenes coding putative c-type lectin domains (CTLD) were found in the species bearing photosynthetic symbionts (A. elongata, Collozoum sp., and S. streptacantha) but not in the non-symbiotic one (A. scolymantha).More particularly, phylogenetic analyses group CTLDs from A. elongata and Collozoum sp. on a distinct branch from S. streptacantha CTLDs, which contained carbohydrate-binding motifs typically observed in other marine photosymbiosis.Our data suggest that similarly to other well-known marine photosymbiosis involving metazoans, the interactions of glycans with c-type lectins is likely involved in modulation of the host/symbiont specific recognition in Radiolaria.

View Article: PubMed Central - PubMed

Affiliation: UMR 7144, Université Pierre et Marie Curie Université Paris 06, Sorbonne Universités, Station Biologique de Roscoff Roscoff, France ; Centre National de la Recherche Scientifique, UMR 7144, Station Biologique de Roscoff Roscoff, France.

ABSTRACT
Rhizaria are an important component of oceanic plankton communities worldwide. A number of species harbor eukaryotic microalgal symbionts, which are horizontally acquired in the environment at each generation. Although these photosymbioses are determinant for Rhizaria ability to thrive in oceanic ecosystems, the mechanisms for symbiotic interactions are unclear. Using high-throughput sequencing technology (i.e., 454), we generated large Expressed Sequence Tag (EST) datasets from four uncultured Rhizaria, an acantharian (Amphilonche elongata), two polycystines (Collozoum sp. and Spongosphaera streptacantha), and one phaeodarian (Aulacantha scolymantha). We assessed the main genetic features of the host/symbionts consortium (i.e., the holobiont) transcriptomes and found rRNA sequences affiliated to a wide range of bacteria and protists in all samples, suggesting that diverse microbial communities are associated with the holobionts. A particular focus was then carried out to search for genes potentially involved in symbiotic processes such as the presence of c-type lectins-coding genes, which are proteins that play a role in cell recognition among eukaryotes. Unigenes coding putative c-type lectin domains (CTLD) were found in the species bearing photosynthetic symbionts (A. elongata, Collozoum sp., and S. streptacantha) but not in the non-symbiotic one (A. scolymantha). More particularly, phylogenetic analyses group CTLDs from A. elongata and Collozoum sp. on a distinct branch from S. streptacantha CTLDs, which contained carbohydrate-binding motifs typically observed in other marine photosymbiosis. Our data suggest that similarly to other well-known marine photosymbiosis involving metazoans, the interactions of glycans with c-type lectins is likely involved in modulation of the host/symbiont specific recognition in Radiolaria.

No MeSH data available.