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Isolation and characterization of plant growth-promoting rhizobacteria from wheat rhizosphere and their effect on plant growth promotion.

Majeed A, Abbasi MK, Hameed S, Imran A, Rahim N - Front Microbiol (2015)

Bottom Line: Nine bacterial isolates were purified, screened in vitro for PGP characteristics and evaluated for their beneficial effects on the early growth of wheat (Triticum aestivum L.).Plant inoculation studies indicated that these Plant growth-promoting rhizobacteria (PGPR) strains provided a significant increase in shoot and root length, and shoot and root biomass.The study is the first report of wheat associated bacterial diversity in the Himalayan region of Rawalakot, AJK.

View Article: PubMed Central - PubMed

Affiliation: Department of Soil and Environmental Sciences, The University of Poonch Rawalakot, Azad Jammu and Kashmir, Pakistan.

ABSTRACT
The present study was conducted to characterize the native plant growth promoting (PGP) bacteria from wheat rhizosphere and root-endosphere in the Himalayan region of Rawalakot, Azad Jammu and Kashmir (AJK), Pakistan. Nine bacterial isolates were purified, screened in vitro for PGP characteristics and evaluated for their beneficial effects on the early growth of wheat (Triticum aestivum L.). Among nine bacterial isolates, seven were able to produce indole-3- acetic acid in tryptophan-supplemented medium; seven were nitrogen fixer, and four were able to solubilize inorganic phosphate in vitro. Four different morphotypes were genotypically identified based on IGS-RFLP fingerprinting and representative of each morphotype was identified by 16S rRNA gene sequencing analysis except Gram-positive putative Bacillus sp. Based on 16S rRNA gene sequence analysis, bacterial isolates AJK-3 and AJK-9 showing multiple PGP-traits were identified as Stenotrophomonas spp. while AJK-7 showed equal homologies to Acetobacter pasteurianus and Stenotrophomonas specie. Plant inoculation studies indicated that these Plant growth-promoting rhizobacteria (PGPR) strains provided a significant increase in shoot and root length, and shoot and root biomass. A significant increase in shoot N contents (up to 76%) and root N contents (up to 32%) was observed over the un-inoculated control. The study indicates the potential of these PGPR for inoculums production or biofertilizers for enhancing growth and nutrient content of wheat and other crops under field conditions. The study is the first report of wheat associated bacterial diversity in the Himalayan region of Rawalakot, AJK.

No MeSH data available.


Effect of PGPR inoculation on shoot and root length (A) and shoot/root dry weight (B) of wheat variety Inqlab grown in growth pouches under axenic conditions. The error bars represent the least significant difference among treatments at P≤ 0.05.
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Figure 3: Effect of PGPR inoculation on shoot and root length (A) and shoot/root dry weight (B) of wheat variety Inqlab grown in growth pouches under axenic conditions. The error bars represent the least significant difference among treatments at P≤ 0.05.

Mentions: The bacterial isolates exerted a significant influence on wheat growth characteristics (Table 3; Figure 3). Comparisons were made among Azospirillum (ER-20) inoculated positive control and a non-inoculated control (with Hoagland N and P). The relative increase in shoot and root length due to bacterial isolates ranged between 25–45% and 29–52%, respectively, over the un-inoculated control whilst the corresponding increase in the shoot and root biomass ranged between 2–62% and 100–172%, respectively (Figure 3A,B). Similarly, bacterial isolates significantly increased N content both in shoot and root compared to un-inoculated control and Er-20 (Figure 4A). The relative increase varied between 22–76% for shoot and 10–32% for root over the un-inoculated control. The correlation between root length and shoot N contents was positive and significant, i.e., R2 = 0.67 (Figure 4B). The efficacy of different isolates for growth characteristics was variable. Bacterial isolates AJK-2, AJK-9, AJK-3, and AJK-7 performed significantly better than others. Overall, the effect of bacterial inoculation was more pronounced on root than shoot.


Isolation and characterization of plant growth-promoting rhizobacteria from wheat rhizosphere and their effect on plant growth promotion.

Majeed A, Abbasi MK, Hameed S, Imran A, Rahim N - Front Microbiol (2015)

Effect of PGPR inoculation on shoot and root length (A) and shoot/root dry weight (B) of wheat variety Inqlab grown in growth pouches under axenic conditions. The error bars represent the least significant difference among treatments at P≤ 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4362341&req=5

Figure 3: Effect of PGPR inoculation on shoot and root length (A) and shoot/root dry weight (B) of wheat variety Inqlab grown in growth pouches under axenic conditions. The error bars represent the least significant difference among treatments at P≤ 0.05.
Mentions: The bacterial isolates exerted a significant influence on wheat growth characteristics (Table 3; Figure 3). Comparisons were made among Azospirillum (ER-20) inoculated positive control and a non-inoculated control (with Hoagland N and P). The relative increase in shoot and root length due to bacterial isolates ranged between 25–45% and 29–52%, respectively, over the un-inoculated control whilst the corresponding increase in the shoot and root biomass ranged between 2–62% and 100–172%, respectively (Figure 3A,B). Similarly, bacterial isolates significantly increased N content both in shoot and root compared to un-inoculated control and Er-20 (Figure 4A). The relative increase varied between 22–76% for shoot and 10–32% for root over the un-inoculated control. The correlation between root length and shoot N contents was positive and significant, i.e., R2 = 0.67 (Figure 4B). The efficacy of different isolates for growth characteristics was variable. Bacterial isolates AJK-2, AJK-9, AJK-3, and AJK-7 performed significantly better than others. Overall, the effect of bacterial inoculation was more pronounced on root than shoot.

Bottom Line: Nine bacterial isolates were purified, screened in vitro for PGP characteristics and evaluated for their beneficial effects on the early growth of wheat (Triticum aestivum L.).Plant inoculation studies indicated that these Plant growth-promoting rhizobacteria (PGPR) strains provided a significant increase in shoot and root length, and shoot and root biomass.The study is the first report of wheat associated bacterial diversity in the Himalayan region of Rawalakot, AJK.

View Article: PubMed Central - PubMed

Affiliation: Department of Soil and Environmental Sciences, The University of Poonch Rawalakot, Azad Jammu and Kashmir, Pakistan.

ABSTRACT
The present study was conducted to characterize the native plant growth promoting (PGP) bacteria from wheat rhizosphere and root-endosphere in the Himalayan region of Rawalakot, Azad Jammu and Kashmir (AJK), Pakistan. Nine bacterial isolates were purified, screened in vitro for PGP characteristics and evaluated for their beneficial effects on the early growth of wheat (Triticum aestivum L.). Among nine bacterial isolates, seven were able to produce indole-3- acetic acid in tryptophan-supplemented medium; seven were nitrogen fixer, and four were able to solubilize inorganic phosphate in vitro. Four different morphotypes were genotypically identified based on IGS-RFLP fingerprinting and representative of each morphotype was identified by 16S rRNA gene sequencing analysis except Gram-positive putative Bacillus sp. Based on 16S rRNA gene sequence analysis, bacterial isolates AJK-3 and AJK-9 showing multiple PGP-traits were identified as Stenotrophomonas spp. while AJK-7 showed equal homologies to Acetobacter pasteurianus and Stenotrophomonas specie. Plant inoculation studies indicated that these Plant growth-promoting rhizobacteria (PGPR) strains provided a significant increase in shoot and root length, and shoot and root biomass. A significant increase in shoot N contents (up to 76%) and root N contents (up to 32%) was observed over the un-inoculated control. The study indicates the potential of these PGPR for inoculums production or biofertilizers for enhancing growth and nutrient content of wheat and other crops under field conditions. The study is the first report of wheat associated bacterial diversity in the Himalayan region of Rawalakot, AJK.

No MeSH data available.