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Isolation and characterization of plant growth-promoting rhizobacteria from wheat rhizosphere and their effect on plant growth promotion.

Majeed A, Abbasi MK, Hameed S, Imran A, Rahim N - Front Microbiol (2015)

Bottom Line: Nine bacterial isolates were purified, screened in vitro for PGP characteristics and evaluated for their beneficial effects on the early growth of wheat (Triticum aestivum L.).Plant inoculation studies indicated that these Plant growth-promoting rhizobacteria (PGPR) strains provided a significant increase in shoot and root length, and shoot and root biomass.The study is the first report of wheat associated bacterial diversity in the Himalayan region of Rawalakot, AJK.

View Article: PubMed Central - PubMed

Affiliation: Department of Soil and Environmental Sciences, The University of Poonch Rawalakot, Azad Jammu and Kashmir, Pakistan.

ABSTRACT
The present study was conducted to characterize the native plant growth promoting (PGP) bacteria from wheat rhizosphere and root-endosphere in the Himalayan region of Rawalakot, Azad Jammu and Kashmir (AJK), Pakistan. Nine bacterial isolates were purified, screened in vitro for PGP characteristics and evaluated for their beneficial effects on the early growth of wheat (Triticum aestivum L.). Among nine bacterial isolates, seven were able to produce indole-3- acetic acid in tryptophan-supplemented medium; seven were nitrogen fixer, and four were able to solubilize inorganic phosphate in vitro. Four different morphotypes were genotypically identified based on IGS-RFLP fingerprinting and representative of each morphotype was identified by 16S rRNA gene sequencing analysis except Gram-positive putative Bacillus sp. Based on 16S rRNA gene sequence analysis, bacterial isolates AJK-3 and AJK-9 showing multiple PGP-traits were identified as Stenotrophomonas spp. while AJK-7 showed equal homologies to Acetobacter pasteurianus and Stenotrophomonas specie. Plant inoculation studies indicated that these Plant growth-promoting rhizobacteria (PGPR) strains provided a significant increase in shoot and root length, and shoot and root biomass. A significant increase in shoot N contents (up to 76%) and root N contents (up to 32%) was observed over the un-inoculated control. The study indicates the potential of these PGPR for inoculums production or biofertilizers for enhancing growth and nutrient content of wheat and other crops under field conditions. The study is the first report of wheat associated bacterial diversity in the Himalayan region of Rawalakot, AJK.

No MeSH data available.


Related in: MedlinePlus

Population density of different bacteria inoculated to wheat at different time intervals under axenic conditions. The error bars represent the least significant difference among treatments at P≤ 0.05.
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Figure 2: Population density of different bacteria inoculated to wheat at different time intervals under axenic conditions. The error bars represent the least significant difference among treatments at P≤ 0.05.

Mentions: Bacterial population size was determined by plate count method on LB agar at different time intervals. We observed that all rhizospheric as well as endophytic bacteria were able to colonize wheat plant roots and showed persistence in the rhizosphere up to 60 days after inoculation (Figure 2). Plants were grown in growth pouches for this experiment; hence, we could not determine the population size at maturity. Maximum colonization was recorded between 30 and 45 days post inoculation. Rhizosphere strain AJK-3 showed maximum number of bacteria at all times as compared to other bacterial isolates.


Isolation and characterization of plant growth-promoting rhizobacteria from wheat rhizosphere and their effect on plant growth promotion.

Majeed A, Abbasi MK, Hameed S, Imran A, Rahim N - Front Microbiol (2015)

Population density of different bacteria inoculated to wheat at different time intervals under axenic conditions. The error bars represent the least significant difference among treatments at P≤ 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4362341&req=5

Figure 2: Population density of different bacteria inoculated to wheat at different time intervals under axenic conditions. The error bars represent the least significant difference among treatments at P≤ 0.05.
Mentions: Bacterial population size was determined by plate count method on LB agar at different time intervals. We observed that all rhizospheric as well as endophytic bacteria were able to colonize wheat plant roots and showed persistence in the rhizosphere up to 60 days after inoculation (Figure 2). Plants were grown in growth pouches for this experiment; hence, we could not determine the population size at maturity. Maximum colonization was recorded between 30 and 45 days post inoculation. Rhizosphere strain AJK-3 showed maximum number of bacteria at all times as compared to other bacterial isolates.

Bottom Line: Nine bacterial isolates were purified, screened in vitro for PGP characteristics and evaluated for their beneficial effects on the early growth of wheat (Triticum aestivum L.).Plant inoculation studies indicated that these Plant growth-promoting rhizobacteria (PGPR) strains provided a significant increase in shoot and root length, and shoot and root biomass.The study is the first report of wheat associated bacterial diversity in the Himalayan region of Rawalakot, AJK.

View Article: PubMed Central - PubMed

Affiliation: Department of Soil and Environmental Sciences, The University of Poonch Rawalakot, Azad Jammu and Kashmir, Pakistan.

ABSTRACT
The present study was conducted to characterize the native plant growth promoting (PGP) bacteria from wheat rhizosphere and root-endosphere in the Himalayan region of Rawalakot, Azad Jammu and Kashmir (AJK), Pakistan. Nine bacterial isolates were purified, screened in vitro for PGP characteristics and evaluated for their beneficial effects on the early growth of wheat (Triticum aestivum L.). Among nine bacterial isolates, seven were able to produce indole-3- acetic acid in tryptophan-supplemented medium; seven were nitrogen fixer, and four were able to solubilize inorganic phosphate in vitro. Four different morphotypes were genotypically identified based on IGS-RFLP fingerprinting and representative of each morphotype was identified by 16S rRNA gene sequencing analysis except Gram-positive putative Bacillus sp. Based on 16S rRNA gene sequence analysis, bacterial isolates AJK-3 and AJK-9 showing multiple PGP-traits were identified as Stenotrophomonas spp. while AJK-7 showed equal homologies to Acetobacter pasteurianus and Stenotrophomonas specie. Plant inoculation studies indicated that these Plant growth-promoting rhizobacteria (PGPR) strains provided a significant increase in shoot and root length, and shoot and root biomass. A significant increase in shoot N contents (up to 76%) and root N contents (up to 32%) was observed over the un-inoculated control. The study indicates the potential of these PGPR for inoculums production or biofertilizers for enhancing growth and nutrient content of wheat and other crops under field conditions. The study is the first report of wheat associated bacterial diversity in the Himalayan region of Rawalakot, AJK.

No MeSH data available.


Related in: MedlinePlus