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Isolation and characterization of plant growth-promoting rhizobacteria from wheat rhizosphere and their effect on plant growth promotion.

Majeed A, Abbasi MK, Hameed S, Imran A, Rahim N - Front Microbiol (2015)

Bottom Line: Nine bacterial isolates were purified, screened in vitro for PGP characteristics and evaluated for their beneficial effects on the early growth of wheat (Triticum aestivum L.).Plant inoculation studies indicated that these Plant growth-promoting rhizobacteria (PGPR) strains provided a significant increase in shoot and root length, and shoot and root biomass.The study is the first report of wheat associated bacterial diversity in the Himalayan region of Rawalakot, AJK.

View Article: PubMed Central - PubMed

Affiliation: Department of Soil and Environmental Sciences, The University of Poonch Rawalakot, Azad Jammu and Kashmir, Pakistan.

ABSTRACT
The present study was conducted to characterize the native plant growth promoting (PGP) bacteria from wheat rhizosphere and root-endosphere in the Himalayan region of Rawalakot, Azad Jammu and Kashmir (AJK), Pakistan. Nine bacterial isolates were purified, screened in vitro for PGP characteristics and evaluated for their beneficial effects on the early growth of wheat (Triticum aestivum L.). Among nine bacterial isolates, seven were able to produce indole-3- acetic acid in tryptophan-supplemented medium; seven were nitrogen fixer, and four were able to solubilize inorganic phosphate in vitro. Four different morphotypes were genotypically identified based on IGS-RFLP fingerprinting and representative of each morphotype was identified by 16S rRNA gene sequencing analysis except Gram-positive putative Bacillus sp. Based on 16S rRNA gene sequence analysis, bacterial isolates AJK-3 and AJK-9 showing multiple PGP-traits were identified as Stenotrophomonas spp. while AJK-7 showed equal homologies to Acetobacter pasteurianus and Stenotrophomonas specie. Plant inoculation studies indicated that these Plant growth-promoting rhizobacteria (PGPR) strains provided a significant increase in shoot and root length, and shoot and root biomass. A significant increase in shoot N contents (up to 76%) and root N contents (up to 32%) was observed over the un-inoculated control. The study indicates the potential of these PGPR for inoculums production or biofertilizers for enhancing growth and nutrient content of wheat and other crops under field conditions. The study is the first report of wheat associated bacterial diversity in the Himalayan region of Rawalakot, AJK.

No MeSH data available.


Related in: MedlinePlus

Solubilization of inorganic tri-calcium phosphate in vitro by bacterial isolate AJK-8 after 7 days of growth at 28 ± 2∘C. The formation of halo zone around the colonies shows the solubilization of the inorganic phosphate.
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Figure 1: Solubilization of inorganic tri-calcium phosphate in vitro by bacterial isolate AJK-8 after 7 days of growth at 28 ± 2∘C. The formation of halo zone around the colonies shows the solubilization of the inorganic phosphate.

Mentions: Four bacteria including three rhizospheric (AJK-1, AJK-2, AJK-3) and one endophytic (AJK-8) were able to form clear zone on Pikovskaia’s agar plates after 7 days of incubation. When quantified spectrophotometrically, maximum P was solubilized by putative Bacillus sp. AJK-8 which was an endophytic strain and formed a clear zone of 3 mm diameter on Pikovskaia’s agar plate (Figure 1). The range of P-solubilization varied from 2 to 19 μg/mL. We could not found any bacteria able to solubilized zinc oxide or zinc sulfate during this study.


Isolation and characterization of plant growth-promoting rhizobacteria from wheat rhizosphere and their effect on plant growth promotion.

Majeed A, Abbasi MK, Hameed S, Imran A, Rahim N - Front Microbiol (2015)

Solubilization of inorganic tri-calcium phosphate in vitro by bacterial isolate AJK-8 after 7 days of growth at 28 ± 2∘C. The formation of halo zone around the colonies shows the solubilization of the inorganic phosphate.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4362341&req=5

Figure 1: Solubilization of inorganic tri-calcium phosphate in vitro by bacterial isolate AJK-8 after 7 days of growth at 28 ± 2∘C. The formation of halo zone around the colonies shows the solubilization of the inorganic phosphate.
Mentions: Four bacteria including three rhizospheric (AJK-1, AJK-2, AJK-3) and one endophytic (AJK-8) were able to form clear zone on Pikovskaia’s agar plates after 7 days of incubation. When quantified spectrophotometrically, maximum P was solubilized by putative Bacillus sp. AJK-8 which was an endophytic strain and formed a clear zone of 3 mm diameter on Pikovskaia’s agar plate (Figure 1). The range of P-solubilization varied from 2 to 19 μg/mL. We could not found any bacteria able to solubilized zinc oxide or zinc sulfate during this study.

Bottom Line: Nine bacterial isolates were purified, screened in vitro for PGP characteristics and evaluated for their beneficial effects on the early growth of wheat (Triticum aestivum L.).Plant inoculation studies indicated that these Plant growth-promoting rhizobacteria (PGPR) strains provided a significant increase in shoot and root length, and shoot and root biomass.The study is the first report of wheat associated bacterial diversity in the Himalayan region of Rawalakot, AJK.

View Article: PubMed Central - PubMed

Affiliation: Department of Soil and Environmental Sciences, The University of Poonch Rawalakot, Azad Jammu and Kashmir, Pakistan.

ABSTRACT
The present study was conducted to characterize the native plant growth promoting (PGP) bacteria from wheat rhizosphere and root-endosphere in the Himalayan region of Rawalakot, Azad Jammu and Kashmir (AJK), Pakistan. Nine bacterial isolates were purified, screened in vitro for PGP characteristics and evaluated for their beneficial effects on the early growth of wheat (Triticum aestivum L.). Among nine bacterial isolates, seven were able to produce indole-3- acetic acid in tryptophan-supplemented medium; seven were nitrogen fixer, and four were able to solubilize inorganic phosphate in vitro. Four different morphotypes were genotypically identified based on IGS-RFLP fingerprinting and representative of each morphotype was identified by 16S rRNA gene sequencing analysis except Gram-positive putative Bacillus sp. Based on 16S rRNA gene sequence analysis, bacterial isolates AJK-3 and AJK-9 showing multiple PGP-traits were identified as Stenotrophomonas spp. while AJK-7 showed equal homologies to Acetobacter pasteurianus and Stenotrophomonas specie. Plant inoculation studies indicated that these Plant growth-promoting rhizobacteria (PGPR) strains provided a significant increase in shoot and root length, and shoot and root biomass. A significant increase in shoot N contents (up to 76%) and root N contents (up to 32%) was observed over the un-inoculated control. The study indicates the potential of these PGPR for inoculums production or biofertilizers for enhancing growth and nutrient content of wheat and other crops under field conditions. The study is the first report of wheat associated bacterial diversity in the Himalayan region of Rawalakot, AJK.

No MeSH data available.


Related in: MedlinePlus