Limits...
Development of a bacterial bioassay for atrazine and cyanuric acid detection.

Hua A, Gueuné H, Cregut M, Thouand G, Durand MJ - Front Microbiol (2015)

Bottom Line: Moreover, atrazine detection by E. coli SM004 ranges from 1.08 to 15 μM.According to WHO recommendations, the cyanuric acid detection range is sensitive enough to discriminate between polluted and drinking water.Nevertheless, the detection of atrazine by E. coli SM004 is only applicable for high concentrations of contaminants.

View Article: PubMed Central - PubMed

Affiliation: Nantes University, Campus de la Courtaisière - IUT, UMR CNRS 6144 GEPEA, CBAC La Roche-sur-Yon, France.

ABSTRACT
The s-triazine herbicides are compounds which can disseminate into soils and water. Due to their toxic effects on living organisms, their concentrations in drinking water are legislated by WHO recommendations. Here we have developed for the first time, to the best of our knowledge, an alternative method for physicochemical quantification using two bioluminescent bacterial biosensors: E. coli SM003 for cyanuric acid detection and E. coli SM004 for both atrazine and cyanuric acid detection. The concentration of cyanuric acid detection for E. coli SM003 ranges from 7.83 μM to 2.89 mM, and for E. coli SM004 ranges from 0.22 to 15 μM. Moreover, atrazine detection by E. coli SM004 ranges from 1.08 to 15 μM. According to WHO recommendations, the cyanuric acid detection range is sensitive enough to discriminate between polluted and drinking water. Nevertheless, the detection of atrazine by E. coli SM004 is only applicable for high concentrations of contaminants.

No MeSH data available.


Related in: MedlinePlus

Atrazine degradation pathway and strategy for atrazine and cyanuric acid detection. In blue are the elements necessary for cyanuric acid detection. In red are the elements necessary for atrazine degradation into cyanuric acid.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4362333&req=5

Figure 1: Atrazine degradation pathway and strategy for atrazine and cyanuric acid detection. In blue are the elements necessary for cyanuric acid detection. In red are the elements necessary for atrazine degradation into cyanuric acid.

Mentions: To address this goal, the pADP-1 plasmid, borne by Pseudomonas sp. pADP-1 and well-studied for its ability to degrade atrazine, was used for genetic construction (Mandelbaum et al., 1995) (Figure 1). Atrazine biodegradation pathway is encoded through the atz genes, which can be divided into two sets: (i) the constitutive atzA, atzB, and atzC genes responsible of atrazine degradation into cyanuric acid and (ii) the inducible atzDEF operon, leading to cyanuric acid mineralization into NH3 and CO2. Moreover, the atzDEF expression requires the constitutive expression of its regulator atzR (Govantes et al., 2010).


Development of a bacterial bioassay for atrazine and cyanuric acid detection.

Hua A, Gueuné H, Cregut M, Thouand G, Durand MJ - Front Microbiol (2015)

Atrazine degradation pathway and strategy for atrazine and cyanuric acid detection. In blue are the elements necessary for cyanuric acid detection. In red are the elements necessary for atrazine degradation into cyanuric acid.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4362333&req=5

Figure 1: Atrazine degradation pathway and strategy for atrazine and cyanuric acid detection. In blue are the elements necessary for cyanuric acid detection. In red are the elements necessary for atrazine degradation into cyanuric acid.
Mentions: To address this goal, the pADP-1 plasmid, borne by Pseudomonas sp. pADP-1 and well-studied for its ability to degrade atrazine, was used for genetic construction (Mandelbaum et al., 1995) (Figure 1). Atrazine biodegradation pathway is encoded through the atz genes, which can be divided into two sets: (i) the constitutive atzA, atzB, and atzC genes responsible of atrazine degradation into cyanuric acid and (ii) the inducible atzDEF operon, leading to cyanuric acid mineralization into NH3 and CO2. Moreover, the atzDEF expression requires the constitutive expression of its regulator atzR (Govantes et al., 2010).

Bottom Line: Moreover, atrazine detection by E. coli SM004 ranges from 1.08 to 15 μM.According to WHO recommendations, the cyanuric acid detection range is sensitive enough to discriminate between polluted and drinking water.Nevertheless, the detection of atrazine by E. coli SM004 is only applicable for high concentrations of contaminants.

View Article: PubMed Central - PubMed

Affiliation: Nantes University, Campus de la Courtaisière - IUT, UMR CNRS 6144 GEPEA, CBAC La Roche-sur-Yon, France.

ABSTRACT
The s-triazine herbicides are compounds which can disseminate into soils and water. Due to their toxic effects on living organisms, their concentrations in drinking water are legislated by WHO recommendations. Here we have developed for the first time, to the best of our knowledge, an alternative method for physicochemical quantification using two bioluminescent bacterial biosensors: E. coli SM003 for cyanuric acid detection and E. coli SM004 for both atrazine and cyanuric acid detection. The concentration of cyanuric acid detection for E. coli SM003 ranges from 7.83 μM to 2.89 mM, and for E. coli SM004 ranges from 0.22 to 15 μM. Moreover, atrazine detection by E. coli SM004 ranges from 1.08 to 15 μM. According to WHO recommendations, the cyanuric acid detection range is sensitive enough to discriminate between polluted and drinking water. Nevertheless, the detection of atrazine by E. coli SM004 is only applicable for high concentrations of contaminants.

No MeSH data available.


Related in: MedlinePlus