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Uncovering the genetic architecture of Colletotrichum lindemuthianum resistance through QTL mapping and epistatic interaction analysis in common bean.

González AM, Yuste-Lisbona FJ, Rodiño AP, De Ron AM, Capel C, García-Alcázar M, Lozano R, Santalla M - Front Plant Sci (2015)

Bottom Line: The homologous genomic regions corresponding to 17 of the 26 main effect QTLs detected were positive for the presence of resistance-associated gene cluster encoding nucleotide-binding and leucine-rich repeat (NL) proteins.Among them, it is worth noting that the main effect QTLs detected on linkage group 05 for resistance to race 1545 in stem, petiole and leaf were located within a 1.2 Mb region.None of the main and epistatic QTLs detected displayed significant environment interaction effects.

View Article: PubMed Central - PubMed

Affiliation: Grupo de Biología de Agrosistemas, Misión Biológica de Galicia-CSIC Pontevedra, Spain.

ABSTRACT
Colletotrichum lindemuthianum is a hemibiotrophic fungal pathogen that causes anthracnose disease in common bean. Despite the genetics of anthracnose resistance has been studied for a long time, few quantitative trait loci (QTLs) studies have been conducted on this species. The present work examines the genetic basis of quantitative resistance to races 23 and 1545 of C. lindemuthianum in different organs (stem, leaf and petiole). A population of 185 recombinant inbred lines (RIL) derived from the cross PMB0225 × PHA1037 was evaluated for anthracnose resistance under natural and artificial photoperiod growth conditions. Using multi-environment QTL mapping approach, 10 and 16 main effect QTLs were identified for resistance to anthracnose races 23 and 1545, respectively. The homologous genomic regions corresponding to 17 of the 26 main effect QTLs detected were positive for the presence of resistance-associated gene cluster encoding nucleotide-binding and leucine-rich repeat (NL) proteins. Among them, it is worth noting that the main effect QTLs detected on linkage group 05 for resistance to race 1545 in stem, petiole and leaf were located within a 1.2 Mb region. The NL gene Phvul.005G117900 is located in this region, which can be considered an important candidate gene for the non-organ-specific QTL identified here. Furthermore, a total of 39 epistatic QTL (E-QTLs) (21 for resistance to race 23 and 18 for resistance to race 1545) involved in 20 epistatic interactions (eleven and nine interactions for resistance to races 23 and 1545, respectively) were identified. None of the main and epistatic QTLs detected displayed significant environment interaction effects. The present research provides essential information not only for the better understanding of the plant-pathogen interaction but also for the application of genomic assisted breeding for anthracnose resistance improvement in common bean through application of marker-assisted selection (MAS).

No MeSH data available.


Related in: MedlinePlus

Location of main effect QTLs for resistance to anthracnose races 23 and 1545 on a genetic linkage map of common bean based on the RIL population developed from the cross PMB0225 × PHA1037. Distances among markers are indicated in cM to the left of the linkage groups (LG); names of markers are shown on the right. QTLs are depicted as vertical bars to the right of the LG. QTL detected for resistance to race 23 are indicated in black. QTLs identified for resistance to race 1545 are shown in gray. Co-location of resistance-associated genes encoding NB-LRR (NL) proteins with anthracnose QTLs are represented to the right of the QTLs.
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Figure 2: Location of main effect QTLs for resistance to anthracnose races 23 and 1545 on a genetic linkage map of common bean based on the RIL population developed from the cross PMB0225 × PHA1037. Distances among markers are indicated in cM to the left of the linkage groups (LG); names of markers are shown on the right. QTLs are depicted as vertical bars to the right of the LG. QTL detected for resistance to race 23 are indicated in black. QTLs identified for resistance to race 1545 are shown in gray. Co-location of resistance-associated genes encoding NB-LRR (NL) proteins with anthracnose QTLs are represented to the right of the QTLs.

Mentions: The evaluation of the RIL population developed from the cross PMB0225 x PHA1037 under different environments has led to the identification of 10 and 16 main effect QTLs involved in resistance against anthracnose races 23 and 1545, respectively. These QTLs were mapped on eight LGs, with the exception of LGs 02, 10, and 11 (Figure 2). All QTLs detected had significant additive effects and did not display significant additive-by-environment interaction effects. A complete report of the single-locus QTL detected for anthracnose resistance traits is given in Table 3.


Uncovering the genetic architecture of Colletotrichum lindemuthianum resistance through QTL mapping and epistatic interaction analysis in common bean.

González AM, Yuste-Lisbona FJ, Rodiño AP, De Ron AM, Capel C, García-Alcázar M, Lozano R, Santalla M - Front Plant Sci (2015)

Location of main effect QTLs for resistance to anthracnose races 23 and 1545 on a genetic linkage map of common bean based on the RIL population developed from the cross PMB0225 × PHA1037. Distances among markers are indicated in cM to the left of the linkage groups (LG); names of markers are shown on the right. QTLs are depicted as vertical bars to the right of the LG. QTL detected for resistance to race 23 are indicated in black. QTLs identified for resistance to race 1545 are shown in gray. Co-location of resistance-associated genes encoding NB-LRR (NL) proteins with anthracnose QTLs are represented to the right of the QTLs.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4362272&req=5

Figure 2: Location of main effect QTLs for resistance to anthracnose races 23 and 1545 on a genetic linkage map of common bean based on the RIL population developed from the cross PMB0225 × PHA1037. Distances among markers are indicated in cM to the left of the linkage groups (LG); names of markers are shown on the right. QTLs are depicted as vertical bars to the right of the LG. QTL detected for resistance to race 23 are indicated in black. QTLs identified for resistance to race 1545 are shown in gray. Co-location of resistance-associated genes encoding NB-LRR (NL) proteins with anthracnose QTLs are represented to the right of the QTLs.
Mentions: The evaluation of the RIL population developed from the cross PMB0225 x PHA1037 under different environments has led to the identification of 10 and 16 main effect QTLs involved in resistance against anthracnose races 23 and 1545, respectively. These QTLs were mapped on eight LGs, with the exception of LGs 02, 10, and 11 (Figure 2). All QTLs detected had significant additive effects and did not display significant additive-by-environment interaction effects. A complete report of the single-locus QTL detected for anthracnose resistance traits is given in Table 3.

Bottom Line: The homologous genomic regions corresponding to 17 of the 26 main effect QTLs detected were positive for the presence of resistance-associated gene cluster encoding nucleotide-binding and leucine-rich repeat (NL) proteins.Among them, it is worth noting that the main effect QTLs detected on linkage group 05 for resistance to race 1545 in stem, petiole and leaf were located within a 1.2 Mb region.None of the main and epistatic QTLs detected displayed significant environment interaction effects.

View Article: PubMed Central - PubMed

Affiliation: Grupo de Biología de Agrosistemas, Misión Biológica de Galicia-CSIC Pontevedra, Spain.

ABSTRACT
Colletotrichum lindemuthianum is a hemibiotrophic fungal pathogen that causes anthracnose disease in common bean. Despite the genetics of anthracnose resistance has been studied for a long time, few quantitative trait loci (QTLs) studies have been conducted on this species. The present work examines the genetic basis of quantitative resistance to races 23 and 1545 of C. lindemuthianum in different organs (stem, leaf and petiole). A population of 185 recombinant inbred lines (RIL) derived from the cross PMB0225 × PHA1037 was evaluated for anthracnose resistance under natural and artificial photoperiod growth conditions. Using multi-environment QTL mapping approach, 10 and 16 main effect QTLs were identified for resistance to anthracnose races 23 and 1545, respectively. The homologous genomic regions corresponding to 17 of the 26 main effect QTLs detected were positive for the presence of resistance-associated gene cluster encoding nucleotide-binding and leucine-rich repeat (NL) proteins. Among them, it is worth noting that the main effect QTLs detected on linkage group 05 for resistance to race 1545 in stem, petiole and leaf were located within a 1.2 Mb region. The NL gene Phvul.005G117900 is located in this region, which can be considered an important candidate gene for the non-organ-specific QTL identified here. Furthermore, a total of 39 epistatic QTL (E-QTLs) (21 for resistance to race 23 and 18 for resistance to race 1545) involved in 20 epistatic interactions (eleven and nine interactions for resistance to races 23 and 1545, respectively) were identified. None of the main and epistatic QTLs detected displayed significant environment interaction effects. The present research provides essential information not only for the better understanding of the plant-pathogen interaction but also for the application of genomic assisted breeding for anthracnose resistance improvement in common bean through application of marker-assisted selection (MAS).

No MeSH data available.


Related in: MedlinePlus