A simple flow cytometry method improves the detection of phosphatidylserine-exposing extracellular vesicles.
Bottom Line: We show that about 50× more Anx5-positive EVs are detected by FCM when detection is triggered on fluorescence as compared with light scattering.By fluorescence triggering, concentrations of 22 000-30 000 Anx5-positive EVs per μL PFP were determined, using two different flow cytometers.Results from EM suggest that EVs down to 100-150 nm diameter are detected by fluorescence triggering.
Affiliation: Molecular Imaging and NanoBioTechnology, UMR-5248-CBMN CNRS-University of Bordeaux-IPB, Pessac, France.Show MeSH
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Mentions: A gallery of EV images obtained by this procedure is presented in Fig.5. Most EVs (Fig.5A–D) present a near-circular shape, as expected from spherical EVs projected onto a flat support. Some EVs present a tubular morphology (Fig.5C,D), while a minor number consists of large fragments and erythrocyte ghosts (Fig.5E).
Affiliation: Molecular Imaging and NanoBioTechnology, UMR-5248-CBMN CNRS-University of Bordeaux-IPB, Pessac, France.