A simple flow cytometry method improves the detection of phosphatidylserine-exposing extracellular vesicles.
Bottom Line: We show that about 50× more Anx5-positive EVs are detected by FCM when detection is triggered on fluorescence as compared with light scattering.This study presents a simple method for enumerating EVs.We believe that this method is applicable in a general context and will improve our understanding of the roles of EVs in pathophysiological situations, which will open avenues for the development of EV-based diagnosis assays.
Affiliation: Molecular Imaging and NanoBioTechnology, UMR-5248-CBMN CNRS-University of Bordeaux-IPB, Pessac, France.Show MeSH
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Mentions: Next, we investigated the influence of Anx5-F* concentration on the detection of Anx5-positive EVs. For both FS and FL triggering, the number of Anx5-positive EVs detected was found to increase with increasing Anx5-F* concentration, until a plateau value was reached (Fig.2). In the conditions used here, namely 10 ×-diluted PFP, the plateau was reached at 0.8 nm Anx5-F*. Most experiments reported in this paper were performed with 2.8 nm Anx5-F*, therefore in saturating conditions.
Affiliation: Molecular Imaging and NanoBioTechnology, UMR-5248-CBMN CNRS-University of Bordeaux-IPB, Pessac, France.