Loss of auditory activity modifies the location of potassium channel KCNQ5 in auditory brainstem neurons.
Bottom Line: The results were comparable after intracochlear TTX injection, which drastically reduced KCNQ5 immunostaining in MNTB calyces and increased immunolabeling in VCN cell bodies.Endbulbs of Held in the VCN also showed diminished KCNQ5 labeling after intracochlear TTX injection.These results show that peripheral activity from auditory nerve afferents is necessary to maintain the subcellular distribution of KCNQ5 in synaptic endings of the auditory brainstem.
Affiliation: Instituto de Investigación en Discapacidades Neurológicas (IDINE), Facultad de Medicina, Universidad de Castilla-La Mancha, Albacete, Spain.Show MeSH
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Mentions: The unilateral TTX injections in the left cochlea resulted in the complete elimination of normal ABR waves after ipsilateral stimulation. Stimulation of the contralateral ear resulted in a slight but not statistically significant increase in the auditory thresholds as well as a modest attenuation of wave amplitudes, particularly in waves III and IV (Fig. 5A). These results support the conclusion that TTX application blocks central auditory responses to sound by blocking auditory nerve activity.
Affiliation: Instituto de Investigación en Discapacidades Neurológicas (IDINE), Facultad de Medicina, Universidad de Castilla-La Mancha, Albacete, Spain.