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Loss of auditory activity modifies the location of potassium channel KCNQ5 in auditory brainstem neurons.

Caminos E, Garcia-Pino E, Juiz JM - J. Neurosci. Res. (2014)

Bottom Line: The results were comparable after intracochlear TTX injection, which drastically reduced KCNQ5 immunostaining in MNTB calyces and increased immunolabeling in VCN cell bodies.Endbulbs of Held in the VCN also showed diminished KCNQ5 labeling after intracochlear TTX injection.These results show that peripheral activity from auditory nerve afferents is necessary to maintain the subcellular distribution of KCNQ5 in synaptic endings of the auditory brainstem.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Investigación en Discapacidades Neurológicas (IDINE), Facultad de Medicina, Universidad de Castilla-La Mancha, Albacete, Spain.

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Representative ABR traces of an adult rat collected before and after cochlear removal. Before cochlear removal, five peak waves in response to click stimuli were recorded in rats at P30. No evoked responses were observed in rats on days 3 (3d) and 40 (40d) after cochlea removal. The sham control did not eliminate the ABR. Sound intensity is indicated (dB SPL). Arrows indicate the start of stimulation.
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fig01: Representative ABR traces of an adult rat collected before and after cochlear removal. Before cochlear removal, five peak waves in response to click stimuli were recorded in rats at P30. No evoked responses were observed in rats on days 3 (3d) and 40 (40d) after cochlea removal. The sham control did not eliminate the ABR. Sound intensity is indicated (dB SPL). Arrows indicate the start of stimulation.

Mentions: ABRs were measured to test whether the bilateral cochlear ablation procedure blocked central auditory activity. Both presurgery and unmanipulated control rats showed that the ABR recordings were within the normal values for Wistar rats, with an average ABR threshold to the click stimuli of 46.5 ± 4.9 dB SPL. Equally, sham surgery had no effect on ABR thresholds or latencies (Fig. 1). After cochlear removal, rats showed a complete elimination of any recordable ABR compared with the same response recorded before cochlear ablation (Fig. 1). These findings indicate that there was no generation of activity driven by auditory nerve input and that brainstem auditory nuclei did not generate activity of this kind after bilateral cochlear ablation at any survival time.


Loss of auditory activity modifies the location of potassium channel KCNQ5 in auditory brainstem neurons.

Caminos E, Garcia-Pino E, Juiz JM - J. Neurosci. Res. (2014)

Representative ABR traces of an adult rat collected before and after cochlear removal. Before cochlear removal, five peak waves in response to click stimuli were recorded in rats at P30. No evoked responses were observed in rats on days 3 (3d) and 40 (40d) after cochlea removal. The sham control did not eliminate the ABR. Sound intensity is indicated (dB SPL). Arrows indicate the start of stimulation.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4359677&req=5

fig01: Representative ABR traces of an adult rat collected before and after cochlear removal. Before cochlear removal, five peak waves in response to click stimuli were recorded in rats at P30. No evoked responses were observed in rats on days 3 (3d) and 40 (40d) after cochlea removal. The sham control did not eliminate the ABR. Sound intensity is indicated (dB SPL). Arrows indicate the start of stimulation.
Mentions: ABRs were measured to test whether the bilateral cochlear ablation procedure blocked central auditory activity. Both presurgery and unmanipulated control rats showed that the ABR recordings were within the normal values for Wistar rats, with an average ABR threshold to the click stimuli of 46.5 ± 4.9 dB SPL. Equally, sham surgery had no effect on ABR thresholds or latencies (Fig. 1). After cochlear removal, rats showed a complete elimination of any recordable ABR compared with the same response recorded before cochlear ablation (Fig. 1). These findings indicate that there was no generation of activity driven by auditory nerve input and that brainstem auditory nuclei did not generate activity of this kind after bilateral cochlear ablation at any survival time.

Bottom Line: The results were comparable after intracochlear TTX injection, which drastically reduced KCNQ5 immunostaining in MNTB calyces and increased immunolabeling in VCN cell bodies.Endbulbs of Held in the VCN also showed diminished KCNQ5 labeling after intracochlear TTX injection.These results show that peripheral activity from auditory nerve afferents is necessary to maintain the subcellular distribution of KCNQ5 in synaptic endings of the auditory brainstem.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Investigación en Discapacidades Neurológicas (IDINE), Facultad de Medicina, Universidad de Castilla-La Mancha, Albacete, Spain.

Show MeSH
Related in: MedlinePlus