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The prognostic value of combined TGF-β1 and ELF in hepatocellular carcinoma.

Ji F, Fu SJ, Shen SL, Zhang LJ, Cao QH, Li SQ, Peng BG, Liang LJ, Hua YP - BMC Cancer (2015)

Bottom Line: The expression of TGF-β1 in HCC tissues was significantly higher than that in normal liver tissues.Data also indicated a significant negative correlation between ELF and TGF-β1.Patients with high TGF-β1 expression or/and low ELF expression appeared to have a poor postoperative disease-free survival and overall survival compared with those with low TGF-β1 expression or/and high ELF expression.

View Article: PubMed Central - PubMed

Affiliation: Organ Transplant Center, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, P. R. China. jifei1215@163.com.

ABSTRACT

Background: Tumor suppression of Transforming Growth Factor (TGF-β) signaling pathway requires an adaptor protein, Embryonic Liver Fodrin (ELF). Disruption of ELF expression resulted in miscolocalization of Smad3 and Smad4, then disruption of TGF-β signaling. However, the prognostic significance of ELF for hepatocellular carcinoma (HCC) hasn't been clarified. This study aimed to investigate whether measuring both TGF-β1 and ELF provides a more powerful predictor for HCC prognosis than either marker alone.

Methods: TGF-β1 and ELF protein were detected by immunohistochemistry. The relationship between TGF-β1/ELF expression and patients' clinicopathologic factors was analyzed. The association between TGF-β1/ELF expression and disease-free survival and overall survival was analyzed by Kaplan-Meier curves, the log-rank test, and Multivariate Cox regression analyses.

Results: The expression of TGF-β1 in HCC tissues was significantly higher than that in normal liver tissues. Conversely, the expression of ELF in HCC tissues declined markedly. ELF protein was correlated with HBsAg, tumor size, tumor number, TNM and recurrence. Data also indicated a significant negative correlation between ELF and TGF-β1. Patients with high TGF-β1 expression or/and low ELF expression appeared to have a poor postoperative disease-free survival and overall survival compared with those with low TGF-β1 expression or/and high ELF expression. Furthermore, the predictive range of ELF combined with TGF-β1 was more sensitive than that of either one alone.

Conclusions: TGF-β1 and ELF protein are potential and reliable biomarkers for predicting prognosis in HCC patients after hepatic resection. Our current study has demonstrated that the prognostic accuracy of testing can be enhanced by their combination.

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Related in: MedlinePlus

Kaplan-Meier curves are shown for time to disease recurrence (A) and overall survival (B) among patients with high or low intratumoral TGF-β1 expression.
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Fig3: Kaplan-Meier curves are shown for time to disease recurrence (A) and overall survival (B) among patients with high or low intratumoral TGF-β1 expression.

Mentions: Similarly, Two groups were divided from 84 HCC patients according to their TGF-β1 expression profiles: the low-expression group (n = 34) and the high-expression group (n = 50). We observed that the 1-, 3- and 5-year DFS rates of the low-expression TGF-β1 group were markedly higher than the high-expression group (79.4%, 73.5% and 62.0% vs 28.0%, 12.0% and 12.0%, respectively, P < 0.001) (Figure 3A). Also, the 1-, 3- and 5-year OS rates of the low-expression TGF-β1 group were significantly higher than those of the high-expression group (94.1%, 85.3% and 76.5% vs 72.0%, 28.0% and 20.0%, respectively, P < 0.001) (Figure 3B). These data suggested that TGF-β1 expression levels were negatively correlated with patients’ DFS and OS.Figure 3


The prognostic value of combined TGF-β1 and ELF in hepatocellular carcinoma.

Ji F, Fu SJ, Shen SL, Zhang LJ, Cao QH, Li SQ, Peng BG, Liang LJ, Hua YP - BMC Cancer (2015)

Kaplan-Meier curves are shown for time to disease recurrence (A) and overall survival (B) among patients with high or low intratumoral TGF-β1 expression.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4359586&req=5

Fig3: Kaplan-Meier curves are shown for time to disease recurrence (A) and overall survival (B) among patients with high or low intratumoral TGF-β1 expression.
Mentions: Similarly, Two groups were divided from 84 HCC patients according to their TGF-β1 expression profiles: the low-expression group (n = 34) and the high-expression group (n = 50). We observed that the 1-, 3- and 5-year DFS rates of the low-expression TGF-β1 group were markedly higher than the high-expression group (79.4%, 73.5% and 62.0% vs 28.0%, 12.0% and 12.0%, respectively, P < 0.001) (Figure 3A). Also, the 1-, 3- and 5-year OS rates of the low-expression TGF-β1 group were significantly higher than those of the high-expression group (94.1%, 85.3% and 76.5% vs 72.0%, 28.0% and 20.0%, respectively, P < 0.001) (Figure 3B). These data suggested that TGF-β1 expression levels were negatively correlated with patients’ DFS and OS.Figure 3

Bottom Line: The expression of TGF-β1 in HCC tissues was significantly higher than that in normal liver tissues.Data also indicated a significant negative correlation between ELF and TGF-β1.Patients with high TGF-β1 expression or/and low ELF expression appeared to have a poor postoperative disease-free survival and overall survival compared with those with low TGF-β1 expression or/and high ELF expression.

View Article: PubMed Central - PubMed

Affiliation: Organ Transplant Center, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, P. R. China. jifei1215@163.com.

ABSTRACT

Background: Tumor suppression of Transforming Growth Factor (TGF-β) signaling pathway requires an adaptor protein, Embryonic Liver Fodrin (ELF). Disruption of ELF expression resulted in miscolocalization of Smad3 and Smad4, then disruption of TGF-β signaling. However, the prognostic significance of ELF for hepatocellular carcinoma (HCC) hasn't been clarified. This study aimed to investigate whether measuring both TGF-β1 and ELF provides a more powerful predictor for HCC prognosis than either marker alone.

Methods: TGF-β1 and ELF protein were detected by immunohistochemistry. The relationship between TGF-β1/ELF expression and patients' clinicopathologic factors was analyzed. The association between TGF-β1/ELF expression and disease-free survival and overall survival was analyzed by Kaplan-Meier curves, the log-rank test, and Multivariate Cox regression analyses.

Results: The expression of TGF-β1 in HCC tissues was significantly higher than that in normal liver tissues. Conversely, the expression of ELF in HCC tissues declined markedly. ELF protein was correlated with HBsAg, tumor size, tumor number, TNM and recurrence. Data also indicated a significant negative correlation between ELF and TGF-β1. Patients with high TGF-β1 expression or/and low ELF expression appeared to have a poor postoperative disease-free survival and overall survival compared with those with low TGF-β1 expression or/and high ELF expression. Furthermore, the predictive range of ELF combined with TGF-β1 was more sensitive than that of either one alone.

Conclusions: TGF-β1 and ELF protein are potential and reliable biomarkers for predicting prognosis in HCC patients after hepatic resection. Our current study has demonstrated that the prognostic accuracy of testing can be enhanced by their combination.

Show MeSH
Related in: MedlinePlus