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The prognostic value of combined TGF-β1 and ELF in hepatocellular carcinoma.

Ji F, Fu SJ, Shen SL, Zhang LJ, Cao QH, Li SQ, Peng BG, Liang LJ, Hua YP - BMC Cancer (2015)

Bottom Line: The expression of TGF-β1 in HCC tissues was significantly higher than that in normal liver tissues.Data also indicated a significant negative correlation between ELF and TGF-β1.Patients with high TGF-β1 expression or/and low ELF expression appeared to have a poor postoperative disease-free survival and overall survival compared with those with low TGF-β1 expression or/and high ELF expression.

View Article: PubMed Central - PubMed

Affiliation: Organ Transplant Center, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, P. R. China. jifei1215@163.com.

ABSTRACT

Background: Tumor suppression of Transforming Growth Factor (TGF-β) signaling pathway requires an adaptor protein, Embryonic Liver Fodrin (ELF). Disruption of ELF expression resulted in miscolocalization of Smad3 and Smad4, then disruption of TGF-β signaling. However, the prognostic significance of ELF for hepatocellular carcinoma (HCC) hasn't been clarified. This study aimed to investigate whether measuring both TGF-β1 and ELF provides a more powerful predictor for HCC prognosis than either marker alone.

Methods: TGF-β1 and ELF protein were detected by immunohistochemistry. The relationship between TGF-β1/ELF expression and patients' clinicopathologic factors was analyzed. The association between TGF-β1/ELF expression and disease-free survival and overall survival was analyzed by Kaplan-Meier curves, the log-rank test, and Multivariate Cox regression analyses.

Results: The expression of TGF-β1 in HCC tissues was significantly higher than that in normal liver tissues. Conversely, the expression of ELF in HCC tissues declined markedly. ELF protein was correlated with HBsAg, tumor size, tumor number, TNM and recurrence. Data also indicated a significant negative correlation between ELF and TGF-β1. Patients with high TGF-β1 expression or/and low ELF expression appeared to have a poor postoperative disease-free survival and overall survival compared with those with low TGF-β1 expression or/and high ELF expression. Furthermore, the predictive range of ELF combined with TGF-β1 was more sensitive than that of either one alone.

Conclusions: TGF-β1 and ELF protein are potential and reliable biomarkers for predicting prognosis in HCC patients after hepatic resection. Our current study has demonstrated that the prognostic accuracy of testing can be enhanced by their combination.

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Related in: MedlinePlus

Kaplan-Meier curves are shown for time to disease recurrence (A) and overall survival (B) among patients with high or low intratumoral ELF expression.
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Fig2: Kaplan-Meier curves are shown for time to disease recurrence (A) and overall survival (B) among patients with high or low intratumoral ELF expression.

Mentions: Firstly, we divided 84 patients with HCC into 2 groups according to their ELF expression profiles: the low-expression group (n = 44) and the high-expression group (n = 40). Using the Kaplan-Meier method to analyze patients’ survival, we found that the 1-, 3- and 5-year DFS rates of the high-expression ELF group were remarkably higher than the low-expression group (75.0%, 60.0% and 57.5% vs 25.0%, 15.9% and 10.2%, respectively, P < 0.001) (Figure 2A), while the 1-, 3- and 5-year OS rates of the high-expression ELF group were significantly higher than those of the low-expression group (90.0%, 72.5% and 65.0% vs 72.7%, 31.8% and 23.7%, respectively, P < 0.001) (Figure 2B). Our findings therefore indicated that ELF expression levels were positively correlated with patients’ DFS and OS.Figure 2


The prognostic value of combined TGF-β1 and ELF in hepatocellular carcinoma.

Ji F, Fu SJ, Shen SL, Zhang LJ, Cao QH, Li SQ, Peng BG, Liang LJ, Hua YP - BMC Cancer (2015)

Kaplan-Meier curves are shown for time to disease recurrence (A) and overall survival (B) among patients with high or low intratumoral ELF expression.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4359586&req=5

Fig2: Kaplan-Meier curves are shown for time to disease recurrence (A) and overall survival (B) among patients with high or low intratumoral ELF expression.
Mentions: Firstly, we divided 84 patients with HCC into 2 groups according to their ELF expression profiles: the low-expression group (n = 44) and the high-expression group (n = 40). Using the Kaplan-Meier method to analyze patients’ survival, we found that the 1-, 3- and 5-year DFS rates of the high-expression ELF group were remarkably higher than the low-expression group (75.0%, 60.0% and 57.5% vs 25.0%, 15.9% and 10.2%, respectively, P < 0.001) (Figure 2A), while the 1-, 3- and 5-year OS rates of the high-expression ELF group were significantly higher than those of the low-expression group (90.0%, 72.5% and 65.0% vs 72.7%, 31.8% and 23.7%, respectively, P < 0.001) (Figure 2B). Our findings therefore indicated that ELF expression levels were positively correlated with patients’ DFS and OS.Figure 2

Bottom Line: The expression of TGF-β1 in HCC tissues was significantly higher than that in normal liver tissues.Data also indicated a significant negative correlation between ELF and TGF-β1.Patients with high TGF-β1 expression or/and low ELF expression appeared to have a poor postoperative disease-free survival and overall survival compared with those with low TGF-β1 expression or/and high ELF expression.

View Article: PubMed Central - PubMed

Affiliation: Organ Transplant Center, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, P. R. China. jifei1215@163.com.

ABSTRACT

Background: Tumor suppression of Transforming Growth Factor (TGF-β) signaling pathway requires an adaptor protein, Embryonic Liver Fodrin (ELF). Disruption of ELF expression resulted in miscolocalization of Smad3 and Smad4, then disruption of TGF-β signaling. However, the prognostic significance of ELF for hepatocellular carcinoma (HCC) hasn't been clarified. This study aimed to investigate whether measuring both TGF-β1 and ELF provides a more powerful predictor for HCC prognosis than either marker alone.

Methods: TGF-β1 and ELF protein were detected by immunohistochemistry. The relationship between TGF-β1/ELF expression and patients' clinicopathologic factors was analyzed. The association between TGF-β1/ELF expression and disease-free survival and overall survival was analyzed by Kaplan-Meier curves, the log-rank test, and Multivariate Cox regression analyses.

Results: The expression of TGF-β1 in HCC tissues was significantly higher than that in normal liver tissues. Conversely, the expression of ELF in HCC tissues declined markedly. ELF protein was correlated with HBsAg, tumor size, tumor number, TNM and recurrence. Data also indicated a significant negative correlation between ELF and TGF-β1. Patients with high TGF-β1 expression or/and low ELF expression appeared to have a poor postoperative disease-free survival and overall survival compared with those with low TGF-β1 expression or/and high ELF expression. Furthermore, the predictive range of ELF combined with TGF-β1 was more sensitive than that of either one alone.

Conclusions: TGF-β1 and ELF protein are potential and reliable biomarkers for predicting prognosis in HCC patients after hepatic resection. Our current study has demonstrated that the prognostic accuracy of testing can be enhanced by their combination.

Show MeSH
Related in: MedlinePlus