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The prognostic value of combined TGF-β1 and ELF in hepatocellular carcinoma.

Ji F, Fu SJ, Shen SL, Zhang LJ, Cao QH, Li SQ, Peng BG, Liang LJ, Hua YP - BMC Cancer (2015)

Bottom Line: The expression of TGF-β1 in HCC tissues was significantly higher than that in normal liver tissues.Data also indicated a significant negative correlation between ELF and TGF-β1.Patients with high TGF-β1 expression or/and low ELF expression appeared to have a poor postoperative disease-free survival and overall survival compared with those with low TGF-β1 expression or/and high ELF expression.

View Article: PubMed Central - PubMed

Affiliation: Organ Transplant Center, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, P. R. China. jifei1215@163.com.

ABSTRACT

Background: Tumor suppression of Transforming Growth Factor (TGF-β) signaling pathway requires an adaptor protein, Embryonic Liver Fodrin (ELF). Disruption of ELF expression resulted in miscolocalization of Smad3 and Smad4, then disruption of TGF-β signaling. However, the prognostic significance of ELF for hepatocellular carcinoma (HCC) hasn't been clarified. This study aimed to investigate whether measuring both TGF-β1 and ELF provides a more powerful predictor for HCC prognosis than either marker alone.

Methods: TGF-β1 and ELF protein were detected by immunohistochemistry. The relationship between TGF-β1/ELF expression and patients' clinicopathologic factors was analyzed. The association between TGF-β1/ELF expression and disease-free survival and overall survival was analyzed by Kaplan-Meier curves, the log-rank test, and Multivariate Cox regression analyses.

Results: The expression of TGF-β1 in HCC tissues was significantly higher than that in normal liver tissues. Conversely, the expression of ELF in HCC tissues declined markedly. ELF protein was correlated with HBsAg, tumor size, tumor number, TNM and recurrence. Data also indicated a significant negative correlation between ELF and TGF-β1. Patients with high TGF-β1 expression or/and low ELF expression appeared to have a poor postoperative disease-free survival and overall survival compared with those with low TGF-β1 expression or/and high ELF expression. Furthermore, the predictive range of ELF combined with TGF-β1 was more sensitive than that of either one alone.

Conclusions: TGF-β1 and ELF protein are potential and reliable biomarkers for predicting prognosis in HCC patients after hepatic resection. Our current study has demonstrated that the prognostic accuracy of testing can be enhanced by their combination.

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Related in: MedlinePlus

Expression of ELF and TGF-β1 protein. (A) Immunohistochemical staining in different tissues is shown. Normal liver tissues (Aa and Ad), HCC adjacent tissues (Ab and Ae), HCC tissues (Ac and Af) (original magnification × 400). (B) and (C) Case distribution of ELF/TGF-β1 expression in normal liver tissues (Normal), HCC adjacent tissues (Para-T) and HCC tissue (Tumor).
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Fig1: Expression of ELF and TGF-β1 protein. (A) Immunohistochemical staining in different tissues is shown. Normal liver tissues (Aa and Ad), HCC adjacent tissues (Ab and Ae), HCC tissues (Ac and Af) (original magnification × 400). (B) and (C) Case distribution of ELF/TGF-β1 expression in normal liver tissues (Normal), HCC adjacent tissues (Para-T) and HCC tissue (Tumor).

Mentions: Using immunohistochemical staining, we examine expression of ELF and TGF-β1 on 20 normal liver tissues, 84 HCC samples and adjacent tissues. All normal liver tissues expressed high level of ELF (20/20). In HCC adjacent tissues, there was a 77.4% high expression rate for ELF (65/84). However, the ELF high expression rate declined to 47.6% (40/84) in HCC tissues. There was significant difference among the groups examined (P < 0.001) (Table 1, Figure 1A, B). On the contrary, the expression rate of TGF-β1 in HCC tissues (59.5%, 50/84) was significantly higher than that in the normal liver tissues (0, 0/20, P < 0.001), but not in HCC adjacent tissues (46.4%, 39/84, P = 0.089, Table 2, Figure 1A, C). These results suggested that there was the low expression of ELF and high expression of TGF-β1 in HCC tissues.Table 1


The prognostic value of combined TGF-β1 and ELF in hepatocellular carcinoma.

Ji F, Fu SJ, Shen SL, Zhang LJ, Cao QH, Li SQ, Peng BG, Liang LJ, Hua YP - BMC Cancer (2015)

Expression of ELF and TGF-β1 protein. (A) Immunohistochemical staining in different tissues is shown. Normal liver tissues (Aa and Ad), HCC adjacent tissues (Ab and Ae), HCC tissues (Ac and Af) (original magnification × 400). (B) and (C) Case distribution of ELF/TGF-β1 expression in normal liver tissues (Normal), HCC adjacent tissues (Para-T) and HCC tissue (Tumor).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4359586&req=5

Fig1: Expression of ELF and TGF-β1 protein. (A) Immunohistochemical staining in different tissues is shown. Normal liver tissues (Aa and Ad), HCC adjacent tissues (Ab and Ae), HCC tissues (Ac and Af) (original magnification × 400). (B) and (C) Case distribution of ELF/TGF-β1 expression in normal liver tissues (Normal), HCC adjacent tissues (Para-T) and HCC tissue (Tumor).
Mentions: Using immunohistochemical staining, we examine expression of ELF and TGF-β1 on 20 normal liver tissues, 84 HCC samples and adjacent tissues. All normal liver tissues expressed high level of ELF (20/20). In HCC adjacent tissues, there was a 77.4% high expression rate for ELF (65/84). However, the ELF high expression rate declined to 47.6% (40/84) in HCC tissues. There was significant difference among the groups examined (P < 0.001) (Table 1, Figure 1A, B). On the contrary, the expression rate of TGF-β1 in HCC tissues (59.5%, 50/84) was significantly higher than that in the normal liver tissues (0, 0/20, P < 0.001), but not in HCC adjacent tissues (46.4%, 39/84, P = 0.089, Table 2, Figure 1A, C). These results suggested that there was the low expression of ELF and high expression of TGF-β1 in HCC tissues.Table 1

Bottom Line: The expression of TGF-β1 in HCC tissues was significantly higher than that in normal liver tissues.Data also indicated a significant negative correlation between ELF and TGF-β1.Patients with high TGF-β1 expression or/and low ELF expression appeared to have a poor postoperative disease-free survival and overall survival compared with those with low TGF-β1 expression or/and high ELF expression.

View Article: PubMed Central - PubMed

Affiliation: Organ Transplant Center, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, P. R. China. jifei1215@163.com.

ABSTRACT

Background: Tumor suppression of Transforming Growth Factor (TGF-β) signaling pathway requires an adaptor protein, Embryonic Liver Fodrin (ELF). Disruption of ELF expression resulted in miscolocalization of Smad3 and Smad4, then disruption of TGF-β signaling. However, the prognostic significance of ELF for hepatocellular carcinoma (HCC) hasn't been clarified. This study aimed to investigate whether measuring both TGF-β1 and ELF provides a more powerful predictor for HCC prognosis than either marker alone.

Methods: TGF-β1 and ELF protein were detected by immunohistochemistry. The relationship between TGF-β1/ELF expression and patients' clinicopathologic factors was analyzed. The association between TGF-β1/ELF expression and disease-free survival and overall survival was analyzed by Kaplan-Meier curves, the log-rank test, and Multivariate Cox regression analyses.

Results: The expression of TGF-β1 in HCC tissues was significantly higher than that in normal liver tissues. Conversely, the expression of ELF in HCC tissues declined markedly. ELF protein was correlated with HBsAg, tumor size, tumor number, TNM and recurrence. Data also indicated a significant negative correlation between ELF and TGF-β1. Patients with high TGF-β1 expression or/and low ELF expression appeared to have a poor postoperative disease-free survival and overall survival compared with those with low TGF-β1 expression or/and high ELF expression. Furthermore, the predictive range of ELF combined with TGF-β1 was more sensitive than that of either one alone.

Conclusions: TGF-β1 and ELF protein are potential and reliable biomarkers for predicting prognosis in HCC patients after hepatic resection. Our current study has demonstrated that the prognostic accuracy of testing can be enhanced by their combination.

Show MeSH
Related in: MedlinePlus