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Wnt antagonist, secreted frizzled-related protein 1, is involved in prenatal skeletal muscle development and is a target of miRNA-1/206 in pigs.

Yang Y, Sun W, Wang R, Lei C, Zhou R, Tang Z, Li K - BMC Mol. Biol. (2015)

Bottom Line: The expression level of the SFRP1 was significantly higher in the embryonic skeletal compared with postnatal skeletal muscle, whereas miR-206 showed the inverse pattern of expression.Dual luciferase assay and Western-blot results demonstrated that SFRP1 was a target of miR-1/206 in porcine iliac endothelial cells.Our results indicate that the SFRP1 gene is regulated by miR-1/206 and potentially affects skeletal muscle development.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, P.R. China. yangyalan1988@126.com.

ABSTRACT

Background: The Wnt signaling pathway is involved in the control of cell proliferation and differentiation during skeletal muscle development. Secreted frizzled-related proteins (SFRPs), such as SFRP1, function as inhibitors of Wnt signaling. MicroRNA-1/206(miRNA-1/206) is specifically expressed in skeletal muscle and play a critical role in myogenesis. The miRNA-mRNA profiles and bioinformatics study suggested that the SFRP1 gene was potentially regulated by miRNA-1/206 during porcine skeletal muscle development.

Methods: To understand the function of SFRP1 and miRNA-1/206 in swine myogenesis, we first predicted the targets of miRNA-1/206 with the TargetScan and PicTar programs, and analyzed the molecular characterization of the porcine SFRP1 gene. We performed a temporal-spatial expression analysis of SFRP1 mRNA and miRNA-206 in Tongcheng pigs (a Chinese indigenous breed) by quantitative real-time polymerase chain reaction, and conducted the co-expression analyses of SFRP1 and miRNA-1/206. Subsequently, the interaction between SFRP1 and miRNA-1/206 was validated via dual luciferase and Western blot assays.

Results: The bioinformatics analysis predicted SFRP1 to be a target of miRNA-1/206. The expression level of the SFRP1 was highly varied across numerous pig tissues and it was down-regulated during porcine skeletal muscle development. The expression level of the SFRP1 was significantly higher in the embryonic skeletal compared with postnatal skeletal muscle, whereas miR-206 showed the inverse pattern of expression. A significant negative correlation was observed between the expression of miR-1/206 and SFRP1 during porcine skeletal muscle development (p <0.05). Dual luciferase assay and Western-blot results demonstrated that SFRP1 was a target of miR-1/206 in porcine iliac endothelial cells.

Conclusions: Our results indicate that the SFRP1 gene is regulated by miR-1/206 and potentially affects skeletal muscle development. These findings increase understanding of the biological functions and the regulation of the SFRP1 gene in mammals.

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Correlation analyses of miRNA-1/206 andSFRP1expression. (A) Pearson’s correlation between SFRP1 and miRNA-1 expression in different tissues. (B) Pearson’s correlation between SFRP1 and miRNA-206 expression in different tissues. (C) Pearson’s correlation between SFRP1 and miRNA-1 expression during skeletal muscle development. (D) Pearson’s correlation between SFRP1 and miRNA-206 expression during skeletal muscle development.
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Fig5: Correlation analyses of miRNA-1/206 andSFRP1expression. (A) Pearson’s correlation between SFRP1 and miRNA-1 expression in different tissues. (B) Pearson’s correlation between SFRP1 and miRNA-206 expression in different tissues. (C) Pearson’s correlation between SFRP1 and miRNA-1 expression during skeletal muscle development. (D) Pearson’s correlation between SFRP1 and miRNA-206 expression during skeletal muscle development.

Mentions: Tang et al. have explored the spatial and dynamic expression of miRNA-1 in the Tongcheng pig (Figure 4D) [35]. According to their study and this study, miRNA-1 and miRNA-206 were abundantly expressed in skeletal muscle and weakly expressed in other tissues. In contrast, SFRP1 was expressed at very low levels in adult skeletal muscle. The co-expression analysis revealed that the SFPR1 mRNA was significantly negatively correlated with miRNA-1 (Pearson’s RSFRP1/miRNA-1 = −0.928, p value = 0.003) (Figure 5A) and miRNA-206 (Pearson’s RSFRP1/miRNA-1 = −0.922, p value = 0.003) (Figure 5B) at the mRNA level in different tissues adult tissues.Figure 5


Wnt antagonist, secreted frizzled-related protein 1, is involved in prenatal skeletal muscle development and is a target of miRNA-1/206 in pigs.

Yang Y, Sun W, Wang R, Lei C, Zhou R, Tang Z, Li K - BMC Mol. Biol. (2015)

Correlation analyses of miRNA-1/206 andSFRP1expression. (A) Pearson’s correlation between SFRP1 and miRNA-1 expression in different tissues. (B) Pearson’s correlation between SFRP1 and miRNA-206 expression in different tissues. (C) Pearson’s correlation between SFRP1 and miRNA-1 expression during skeletal muscle development. (D) Pearson’s correlation between SFRP1 and miRNA-206 expression during skeletal muscle development.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4359577&req=5

Fig5: Correlation analyses of miRNA-1/206 andSFRP1expression. (A) Pearson’s correlation between SFRP1 and miRNA-1 expression in different tissues. (B) Pearson’s correlation between SFRP1 and miRNA-206 expression in different tissues. (C) Pearson’s correlation between SFRP1 and miRNA-1 expression during skeletal muscle development. (D) Pearson’s correlation between SFRP1 and miRNA-206 expression during skeletal muscle development.
Mentions: Tang et al. have explored the spatial and dynamic expression of miRNA-1 in the Tongcheng pig (Figure 4D) [35]. According to their study and this study, miRNA-1 and miRNA-206 were abundantly expressed in skeletal muscle and weakly expressed in other tissues. In contrast, SFRP1 was expressed at very low levels in adult skeletal muscle. The co-expression analysis revealed that the SFPR1 mRNA was significantly negatively correlated with miRNA-1 (Pearson’s RSFRP1/miRNA-1 = −0.928, p value = 0.003) (Figure 5A) and miRNA-206 (Pearson’s RSFRP1/miRNA-1 = −0.922, p value = 0.003) (Figure 5B) at the mRNA level in different tissues adult tissues.Figure 5

Bottom Line: The expression level of the SFRP1 was significantly higher in the embryonic skeletal compared with postnatal skeletal muscle, whereas miR-206 showed the inverse pattern of expression.Dual luciferase assay and Western-blot results demonstrated that SFRP1 was a target of miR-1/206 in porcine iliac endothelial cells.Our results indicate that the SFRP1 gene is regulated by miR-1/206 and potentially affects skeletal muscle development.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, P.R. China. yangyalan1988@126.com.

ABSTRACT

Background: The Wnt signaling pathway is involved in the control of cell proliferation and differentiation during skeletal muscle development. Secreted frizzled-related proteins (SFRPs), such as SFRP1, function as inhibitors of Wnt signaling. MicroRNA-1/206(miRNA-1/206) is specifically expressed in skeletal muscle and play a critical role in myogenesis. The miRNA-mRNA profiles and bioinformatics study suggested that the SFRP1 gene was potentially regulated by miRNA-1/206 during porcine skeletal muscle development.

Methods: To understand the function of SFRP1 and miRNA-1/206 in swine myogenesis, we first predicted the targets of miRNA-1/206 with the TargetScan and PicTar programs, and analyzed the molecular characterization of the porcine SFRP1 gene. We performed a temporal-spatial expression analysis of SFRP1 mRNA and miRNA-206 in Tongcheng pigs (a Chinese indigenous breed) by quantitative real-time polymerase chain reaction, and conducted the co-expression analyses of SFRP1 and miRNA-1/206. Subsequently, the interaction between SFRP1 and miRNA-1/206 was validated via dual luciferase and Western blot assays.

Results: The bioinformatics analysis predicted SFRP1 to be a target of miRNA-1/206. The expression level of the SFRP1 was highly varied across numerous pig tissues and it was down-regulated during porcine skeletal muscle development. The expression level of the SFRP1 was significantly higher in the embryonic skeletal compared with postnatal skeletal muscle, whereas miR-206 showed the inverse pattern of expression. A significant negative correlation was observed between the expression of miR-1/206 and SFRP1 during porcine skeletal muscle development (p <0.05). Dual luciferase assay and Western-blot results demonstrated that SFRP1 was a target of miR-1/206 in porcine iliac endothelial cells.

Conclusions: Our results indicate that the SFRP1 gene is regulated by miR-1/206 and potentially affects skeletal muscle development. These findings increase understanding of the biological functions and the regulation of the SFRP1 gene in mammals.

Show MeSH