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2G ethanol from the whole sugarcane lignocellulosic biomass.

Pereira SC, Maehara L, Machado CM, Farinas CS - Biotechnol Biofuels (2015)

Bottom Line: For the four commercial sugarcane varieties evaluated using the same experimental set of conditions, it was found that the variety of sugarcane was not a significant factor in the 2G ethanol production process.Assessment of use of the whole lignocellulosic sugarcane biomass clearly showed that 2G ethanol production could be significantly improved by the combined use of bagasse, straw, and tops, when compared to the use of bagasse alone.Furthermore, given that the variety was not a significant factor for the 2G ethanol production process within the four commercial sugarcane varieties evaluated here, agronomic features such as higher productivity and tolerance of soil and climate variations can be used as the criteria for variety selection.

View Article: PubMed Central - PubMed

Affiliation: Embrapa Instrumentation, Rua XV de Novembro 1452, 13560-970 São Carlos, SP Brazil.

ABSTRACT

Background: In the sugarcane industry, large amounts of lignocellulosic residues are generated, which includes bagasse, straw, and tops. The use of the whole sugarcane lignocellulosic biomass for the production of second-generation (2G) ethanol can be a potential alternative to contribute to the economic viability of this process. Here, we conducted a systematic comparative study of the use of the lignocellulosic residues from the whole sugarcane lignocellulosic biomass (bagasse, straw, and tops) from commercial sugarcane varieties for the production of 2G ethanol. In addition, the feasibility of using a mixture of these residues from a selected variety was also investigated.

Results: The materials were pretreated with dilute acid and hydrolyzed with a commercial enzymatic preparation, after which the hydrolysates were fermented using an industrial strain of Saccharomyces cerevisiae. The susceptibility to enzymatic saccharification was higher for the tops, followed by straw and bagasse. Interestingly, the fermentability of the hydrolysates showed a different profile, with straw achieving the highest ethanol yields, followed by tops and bagasse. Using a mixture of the different sugarcane parts (bagasse-straw-tops, 1:1:1, in a dry-weight basis), it was possible to achieve a 55% higher enzymatic conversion and a 25% higher ethanol yield, compared to use of the bagasse alone. For the four commercial sugarcane varieties evaluated using the same experimental set of conditions, it was found that the variety of sugarcane was not a significant factor in the 2G ethanol production process.

Conclusions: Assessment of use of the whole lignocellulosic sugarcane biomass clearly showed that 2G ethanol production could be significantly improved by the combined use of bagasse, straw, and tops, when compared to the use of bagasse alone. The lower susceptibility to saccharification of sugarcane bagasse, as well as the lower fermentability of its hydrolysates, can be compensated by using it in combination with straw and tops (sugarcane trash). Furthermore, given that the variety was not a significant factor for the 2G ethanol production process within the four commercial sugarcane varieties evaluated here, agronomic features such as higher productivity and tolerance of soil and climate variations can be used as the criteria for variety selection.

No MeSH data available.


Related in: MedlinePlus

Temporal profiles of glucose release and statistical analysis of the data for enzymatic conversion. Comparison of the temporal profiles of glucose release (A) and statistical analysis of the data for enzymatic conversion of cellulose after 24 h (B) for the saccharification of bagasse, straw, tops, and the combination of them (bagasse-straw-tops, 1:1:1 mixture), using variety K. The lines are models fitted according to the Chrastil [61] approach. Means with different capital letters are statistically different (Tukey’s test, P < 0.05).
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Fig4: Temporal profiles of glucose release and statistical analysis of the data for enzymatic conversion. Comparison of the temporal profiles of glucose release (A) and statistical analysis of the data for enzymatic conversion of cellulose after 24 h (B) for the saccharification of bagasse, straw, tops, and the combination of them (bagasse-straw-tops, 1:1:1 mixture), using variety K. The lines are models fitted according to the Chrastil [61] approach. Means with different capital letters are statistically different (Tukey’s test, P < 0.05).

Mentions: After assessing the responses for each type of residue (bagasse, straw, and tops) obtained after processing of the four sugarcane varieties (K, M, Q, and X), investigation was made of the feasibility of using a mixture of the three sugarcane residues for the conversion of cellulose into glucose and ethanol production. For this purpose, variety K was arbitrarily selected, since there were no significant differences among the varieties in terms of enzymatic conversion (Table 4). Figure 4A shows the temporal profiles of glucose release during the enzymatic hydrolysis step for the three separate residues and a mixture of them (bagasse-straw-tops, 1:1:1, dry weight basis). The chemical composition of the pretreated mixture was calculated as a weighted average of the compositions of the individual pretreated residues.Figure 4


2G ethanol from the whole sugarcane lignocellulosic biomass.

Pereira SC, Maehara L, Machado CM, Farinas CS - Biotechnol Biofuels (2015)

Temporal profiles of glucose release and statistical analysis of the data for enzymatic conversion. Comparison of the temporal profiles of glucose release (A) and statistical analysis of the data for enzymatic conversion of cellulose after 24 h (B) for the saccharification of bagasse, straw, tops, and the combination of them (bagasse-straw-tops, 1:1:1 mixture), using variety K. The lines are models fitted according to the Chrastil [61] approach. Means with different capital letters are statistically different (Tukey’s test, P < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4359543&req=5

Fig4: Temporal profiles of glucose release and statistical analysis of the data for enzymatic conversion. Comparison of the temporal profiles of glucose release (A) and statistical analysis of the data for enzymatic conversion of cellulose after 24 h (B) for the saccharification of bagasse, straw, tops, and the combination of them (bagasse-straw-tops, 1:1:1 mixture), using variety K. The lines are models fitted according to the Chrastil [61] approach. Means with different capital letters are statistically different (Tukey’s test, P < 0.05).
Mentions: After assessing the responses for each type of residue (bagasse, straw, and tops) obtained after processing of the four sugarcane varieties (K, M, Q, and X), investigation was made of the feasibility of using a mixture of the three sugarcane residues for the conversion of cellulose into glucose and ethanol production. For this purpose, variety K was arbitrarily selected, since there were no significant differences among the varieties in terms of enzymatic conversion (Table 4). Figure 4A shows the temporal profiles of glucose release during the enzymatic hydrolysis step for the three separate residues and a mixture of them (bagasse-straw-tops, 1:1:1, dry weight basis). The chemical composition of the pretreated mixture was calculated as a weighted average of the compositions of the individual pretreated residues.Figure 4

Bottom Line: For the four commercial sugarcane varieties evaluated using the same experimental set of conditions, it was found that the variety of sugarcane was not a significant factor in the 2G ethanol production process.Assessment of use of the whole lignocellulosic sugarcane biomass clearly showed that 2G ethanol production could be significantly improved by the combined use of bagasse, straw, and tops, when compared to the use of bagasse alone.Furthermore, given that the variety was not a significant factor for the 2G ethanol production process within the four commercial sugarcane varieties evaluated here, agronomic features such as higher productivity and tolerance of soil and climate variations can be used as the criteria for variety selection.

View Article: PubMed Central - PubMed

Affiliation: Embrapa Instrumentation, Rua XV de Novembro 1452, 13560-970 São Carlos, SP Brazil.

ABSTRACT

Background: In the sugarcane industry, large amounts of lignocellulosic residues are generated, which includes bagasse, straw, and tops. The use of the whole sugarcane lignocellulosic biomass for the production of second-generation (2G) ethanol can be a potential alternative to contribute to the economic viability of this process. Here, we conducted a systematic comparative study of the use of the lignocellulosic residues from the whole sugarcane lignocellulosic biomass (bagasse, straw, and tops) from commercial sugarcane varieties for the production of 2G ethanol. In addition, the feasibility of using a mixture of these residues from a selected variety was also investigated.

Results: The materials were pretreated with dilute acid and hydrolyzed with a commercial enzymatic preparation, after which the hydrolysates were fermented using an industrial strain of Saccharomyces cerevisiae. The susceptibility to enzymatic saccharification was higher for the tops, followed by straw and bagasse. Interestingly, the fermentability of the hydrolysates showed a different profile, with straw achieving the highest ethanol yields, followed by tops and bagasse. Using a mixture of the different sugarcane parts (bagasse-straw-tops, 1:1:1, in a dry-weight basis), it was possible to achieve a 55% higher enzymatic conversion and a 25% higher ethanol yield, compared to use of the bagasse alone. For the four commercial sugarcane varieties evaluated using the same experimental set of conditions, it was found that the variety of sugarcane was not a significant factor in the 2G ethanol production process.

Conclusions: Assessment of use of the whole lignocellulosic sugarcane biomass clearly showed that 2G ethanol production could be significantly improved by the combined use of bagasse, straw, and tops, when compared to the use of bagasse alone. The lower susceptibility to saccharification of sugarcane bagasse, as well as the lower fermentability of its hydrolysates, can be compensated by using it in combination with straw and tops (sugarcane trash). Furthermore, given that the variety was not a significant factor for the 2G ethanol production process within the four commercial sugarcane varieties evaluated here, agronomic features such as higher productivity and tolerance of soil and climate variations can be used as the criteria for variety selection.

No MeSH data available.


Related in: MedlinePlus