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Transcriptome responses to heat stress in hypothalamus of a meat-type chicken.

Sun H, Jiang R, Xu S, Zhang Z, Xu G, Zheng J, Qu L - J Anim Sci Biotechnol (2015)

Bottom Line: Consistent expression results were found for 11 selected genes by quantitative real-time PCR.Thirty-eight interesting differential expression genes were found from GO term annotation and those genes were related to meat quality, growth, and crucial enzymes.Moreover, the transcripts of heat-shock protein, including Hsp 40 and Hsp 90, were significantly altered in response to thermal stress.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, 100193 China.

ABSTRACT

Background: Heat stress has resulted in great losses in poultry production. To address this issue, we systematically analyzed chicken hypothalamus transcriptome responses to thermal stress using a 44 k chicken Agilent microarray.

Methods: Hypothalamus samples were collected from a control group reared at 25°C, a heat-stress group treated at 34°C for 24 h, and a temperature-recovery group reared at 25°C for 24 h following a heat-stress treatment. We compared the expression profiles between each pair of the three groups using microarray data.

Results: A total of 1,967 probe sets were found to be differentially expressed in the three comparisons with P < 0.05 and a fold change (FC) higher than 1.5, and the genes were mainly involved in self-regulation and compensation required to maintain homeostasis. Consistent expression results were found for 11 selected genes by quantitative real-time PCR. Thirty-eight interesting differential expression genes were found from GO term annotation and those genes were related to meat quality, growth, and crucial enzymes. Using these genes for genetic network analysis, we obtained three genetic networks. Moreover, the transcripts of heat-shock protein, including Hsp 40 and Hsp 90, were significantly altered in response to thermal stress.

Conclusions: This study provides a broader understanding of molecular mechanisms underlying stress response in chickens and discovery of novel genes that are regulated in a specific thermal-stress manner.

No MeSH data available.


Related in: MedlinePlus

Cardiovascular disease, lipid metabolism, molecular transport. Pathway analysis of gene functions in broiler hypothalamus transcriptome in response to acute heat stress. Red color shows up-regulation and green color shows down-regulation (IPA). The intensity of green and red molecule colors indicates the degree of down or upregulation, respectively. White molecules are not differential expression, but are included to illustrate association with significantly up-regulated and down-regulated genes.
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Fig7: Cardiovascular disease, lipid metabolism, molecular transport. Pathway analysis of gene functions in broiler hypothalamus transcriptome in response to acute heat stress. Red color shows up-regulation and green color shows down-regulation (IPA). The intensity of green and red molecule colors indicates the degree of down or upregulation, respectively. White molecules are not differential expression, but are included to illustrate association with significantly up-regulated and down-regulated genes.

Mentions: Based on the fact that genes might be related to meat quality, growth factor, and enzyme from the GO annotation, a functional network of these DE genes and their interrelationships were analyzed using Ingenuity Pathway Analysis (IPA); three genetic networks (P-value = 0.0001) were created from those DE genes: “Cardiovascular disease, lipid metabolism, molecular transport” (ACTC1, BMP3, CETP, DBI, FABP7, EMP1, FIGF, LIPG, MYH11, PDK4, PTGS2, SDC1, and TH), “Cell-to-cell signalling and interaction, molecular transport, small molecule biochemistry” (ACTC1, AOAH, DRD2, GCH1, GDPD5, JPH1, LMX1A, LOC430178, and UCP3), and “Lipid metabolism, molecular transport, small molecule biochemistry” (CROT, CUBN, DRD2, LPAR4, MYH7, PLA1A, and TH) (Figures 7, 8 and 9).Figure 7


Transcriptome responses to heat stress in hypothalamus of a meat-type chicken.

Sun H, Jiang R, Xu S, Zhang Z, Xu G, Zheng J, Qu L - J Anim Sci Biotechnol (2015)

Cardiovascular disease, lipid metabolism, molecular transport. Pathway analysis of gene functions in broiler hypothalamus transcriptome in response to acute heat stress. Red color shows up-regulation and green color shows down-regulation (IPA). The intensity of green and red molecule colors indicates the degree of down or upregulation, respectively. White molecules are not differential expression, but are included to illustrate association with significantly up-regulated and down-regulated genes.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4359534&req=5

Fig7: Cardiovascular disease, lipid metabolism, molecular transport. Pathway analysis of gene functions in broiler hypothalamus transcriptome in response to acute heat stress. Red color shows up-regulation and green color shows down-regulation (IPA). The intensity of green and red molecule colors indicates the degree of down or upregulation, respectively. White molecules are not differential expression, but are included to illustrate association with significantly up-regulated and down-regulated genes.
Mentions: Based on the fact that genes might be related to meat quality, growth factor, and enzyme from the GO annotation, a functional network of these DE genes and their interrelationships were analyzed using Ingenuity Pathway Analysis (IPA); three genetic networks (P-value = 0.0001) were created from those DE genes: “Cardiovascular disease, lipid metabolism, molecular transport” (ACTC1, BMP3, CETP, DBI, FABP7, EMP1, FIGF, LIPG, MYH11, PDK4, PTGS2, SDC1, and TH), “Cell-to-cell signalling and interaction, molecular transport, small molecule biochemistry” (ACTC1, AOAH, DRD2, GCH1, GDPD5, JPH1, LMX1A, LOC430178, and UCP3), and “Lipid metabolism, molecular transport, small molecule biochemistry” (CROT, CUBN, DRD2, LPAR4, MYH7, PLA1A, and TH) (Figures 7, 8 and 9).Figure 7

Bottom Line: Consistent expression results were found for 11 selected genes by quantitative real-time PCR.Thirty-eight interesting differential expression genes were found from GO term annotation and those genes were related to meat quality, growth, and crucial enzymes.Moreover, the transcripts of heat-shock protein, including Hsp 40 and Hsp 90, were significantly altered in response to thermal stress.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, 100193 China.

ABSTRACT

Background: Heat stress has resulted in great losses in poultry production. To address this issue, we systematically analyzed chicken hypothalamus transcriptome responses to thermal stress using a 44 k chicken Agilent microarray.

Methods: Hypothalamus samples were collected from a control group reared at 25°C, a heat-stress group treated at 34°C for 24 h, and a temperature-recovery group reared at 25°C for 24 h following a heat-stress treatment. We compared the expression profiles between each pair of the three groups using microarray data.

Results: A total of 1,967 probe sets were found to be differentially expressed in the three comparisons with P < 0.05 and a fold change (FC) higher than 1.5, and the genes were mainly involved in self-regulation and compensation required to maintain homeostasis. Consistent expression results were found for 11 selected genes by quantitative real-time PCR. Thirty-eight interesting differential expression genes were found from GO term annotation and those genes were related to meat quality, growth, and crucial enzymes. Using these genes for genetic network analysis, we obtained three genetic networks. Moreover, the transcripts of heat-shock protein, including Hsp 40 and Hsp 90, were significantly altered in response to thermal stress.

Conclusions: This study provides a broader understanding of molecular mechanisms underlying stress response in chickens and discovery of novel genes that are regulated in a specific thermal-stress manner.

No MeSH data available.


Related in: MedlinePlus