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Transcriptome responses to heat stress in hypothalamus of a meat-type chicken.

Sun H, Jiang R, Xu S, Zhang Z, Xu G, Zheng J, Qu L - J Anim Sci Biotechnol (2015)

Bottom Line: Consistent expression results were found for 11 selected genes by quantitative real-time PCR.Thirty-eight interesting differential expression genes were found from GO term annotation and those genes were related to meat quality, growth, and crucial enzymes.Moreover, the transcripts of heat-shock protein, including Hsp 40 and Hsp 90, were significantly altered in response to thermal stress.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, 100193 China.

ABSTRACT

Background: Heat stress has resulted in great losses in poultry production. To address this issue, we systematically analyzed chicken hypothalamus transcriptome responses to thermal stress using a 44 k chicken Agilent microarray.

Methods: Hypothalamus samples were collected from a control group reared at 25°C, a heat-stress group treated at 34°C for 24 h, and a temperature-recovery group reared at 25°C for 24 h following a heat-stress treatment. We compared the expression profiles between each pair of the three groups using microarray data.

Results: A total of 1,967 probe sets were found to be differentially expressed in the three comparisons with P < 0.05 and a fold change (FC) higher than 1.5, and the genes were mainly involved in self-regulation and compensation required to maintain homeostasis. Consistent expression results were found for 11 selected genes by quantitative real-time PCR. Thirty-eight interesting differential expression genes were found from GO term annotation and those genes were related to meat quality, growth, and crucial enzymes. Using these genes for genetic network analysis, we obtained three genetic networks. Moreover, the transcripts of heat-shock protein, including Hsp 40 and Hsp 90, were significantly altered in response to thermal stress.

Conclusions: This study provides a broader understanding of molecular mechanisms underlying stress response in chickens and discovery of novel genes that are regulated in a specific thermal-stress manner.

No MeSH data available.


Related in: MedlinePlus

Gene ontology (GO) annotation of up-regulated differentially expressed genes (P < 0.05).
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Fig6: Gene ontology (GO) annotation of up-regulated differentially expressed genes (P < 0.05).

Mentions: Gene ontology was used to evaluate the function of DE genes in three comparisons (Figures 5 and 6). All the DE genes in HS vs. CL, HS vs. TR, and TR vs. CL were performed by gene ontology (GO) terms through the DAVID platform. The biological process (BP) components were presented as functional clusters. The significantly-enriched GO categories were selected only when P < 0.05 at the fifth level. In summary, up-regulated genes in HS vs. CL and HS vs. TR were mainly enriched in regulation of cell morphogenesis involved in differentiation, neurogenesis, cellular component morphogenesis, neuron development, neuron projection morphogenesis, and transmembrane transport. GO items such as ion transport and cognition were associated only with down-regulation in TR vs. CL. The down-regulated genes between HS vs. CL and TR vs. CL showed muscle-organ development, striated muscle-tissue development, and cardiac muscle-tissue development; muscle-tissue development was enriched, indicating that muscle development is significantly inhibited during heat stress. In HS vs. CL, the up-regulated genes were mostly concentrated in regulation of gene expression, regulation of macromolecule biosynthetic process, regulation of nucleobase, nucleoside, nucleotide and nucleic acid metabolic processes, regulation of transcription, and DNA metabolic processes. The regulation of protein amino acid phosphorylation and lymph vessel development were only changed in HS vs. TR. Also, among the many functional GO annotation categories, a number of genes were involved that might relate to or affect meat quality, growth factor, and enzyme (Additional file 1: Table S1).Figure 5


Transcriptome responses to heat stress in hypothalamus of a meat-type chicken.

Sun H, Jiang R, Xu S, Zhang Z, Xu G, Zheng J, Qu L - J Anim Sci Biotechnol (2015)

Gene ontology (GO) annotation of up-regulated differentially expressed genes (P < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4359534&req=5

Fig6: Gene ontology (GO) annotation of up-regulated differentially expressed genes (P < 0.05).
Mentions: Gene ontology was used to evaluate the function of DE genes in three comparisons (Figures 5 and 6). All the DE genes in HS vs. CL, HS vs. TR, and TR vs. CL were performed by gene ontology (GO) terms through the DAVID platform. The biological process (BP) components were presented as functional clusters. The significantly-enriched GO categories were selected only when P < 0.05 at the fifth level. In summary, up-regulated genes in HS vs. CL and HS vs. TR were mainly enriched in regulation of cell morphogenesis involved in differentiation, neurogenesis, cellular component morphogenesis, neuron development, neuron projection morphogenesis, and transmembrane transport. GO items such as ion transport and cognition were associated only with down-regulation in TR vs. CL. The down-regulated genes between HS vs. CL and TR vs. CL showed muscle-organ development, striated muscle-tissue development, and cardiac muscle-tissue development; muscle-tissue development was enriched, indicating that muscle development is significantly inhibited during heat stress. In HS vs. CL, the up-regulated genes were mostly concentrated in regulation of gene expression, regulation of macromolecule biosynthetic process, regulation of nucleobase, nucleoside, nucleotide and nucleic acid metabolic processes, regulation of transcription, and DNA metabolic processes. The regulation of protein amino acid phosphorylation and lymph vessel development were only changed in HS vs. TR. Also, among the many functional GO annotation categories, a number of genes were involved that might relate to or affect meat quality, growth factor, and enzyme (Additional file 1: Table S1).Figure 5

Bottom Line: Consistent expression results were found for 11 selected genes by quantitative real-time PCR.Thirty-eight interesting differential expression genes were found from GO term annotation and those genes were related to meat quality, growth, and crucial enzymes.Moreover, the transcripts of heat-shock protein, including Hsp 40 and Hsp 90, were significantly altered in response to thermal stress.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, 100193 China.

ABSTRACT

Background: Heat stress has resulted in great losses in poultry production. To address this issue, we systematically analyzed chicken hypothalamus transcriptome responses to thermal stress using a 44 k chicken Agilent microarray.

Methods: Hypothalamus samples were collected from a control group reared at 25°C, a heat-stress group treated at 34°C for 24 h, and a temperature-recovery group reared at 25°C for 24 h following a heat-stress treatment. We compared the expression profiles between each pair of the three groups using microarray data.

Results: A total of 1,967 probe sets were found to be differentially expressed in the three comparisons with P < 0.05 and a fold change (FC) higher than 1.5, and the genes were mainly involved in self-regulation and compensation required to maintain homeostasis. Consistent expression results were found for 11 selected genes by quantitative real-time PCR. Thirty-eight interesting differential expression genes were found from GO term annotation and those genes were related to meat quality, growth, and crucial enzymes. Using these genes for genetic network analysis, we obtained three genetic networks. Moreover, the transcripts of heat-shock protein, including Hsp 40 and Hsp 90, were significantly altered in response to thermal stress.

Conclusions: This study provides a broader understanding of molecular mechanisms underlying stress response in chickens and discovery of novel genes that are regulated in a specific thermal-stress manner.

No MeSH data available.


Related in: MedlinePlus