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Transcriptome responses to heat stress in hypothalamus of a meat-type chicken.

Sun H, Jiang R, Xu S, Zhang Z, Xu G, Zheng J, Qu L - J Anim Sci Biotechnol (2015)

Bottom Line: Consistent expression results were found for 11 selected genes by quantitative real-time PCR.Thirty-eight interesting differential expression genes were found from GO term annotation and those genes were related to meat quality, growth, and crucial enzymes.Moreover, the transcripts of heat-shock protein, including Hsp 40 and Hsp 90, were significantly altered in response to thermal stress.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, 100193 China.

ABSTRACT

Background: Heat stress has resulted in great losses in poultry production. To address this issue, we systematically analyzed chicken hypothalamus transcriptome responses to thermal stress using a 44 k chicken Agilent microarray.

Methods: Hypothalamus samples were collected from a control group reared at 25°C, a heat-stress group treated at 34°C for 24 h, and a temperature-recovery group reared at 25°C for 24 h following a heat-stress treatment. We compared the expression profiles between each pair of the three groups using microarray data.

Results: A total of 1,967 probe sets were found to be differentially expressed in the three comparisons with P < 0.05 and a fold change (FC) higher than 1.5, and the genes were mainly involved in self-regulation and compensation required to maintain homeostasis. Consistent expression results were found for 11 selected genes by quantitative real-time PCR. Thirty-eight interesting differential expression genes were found from GO term annotation and those genes were related to meat quality, growth, and crucial enzymes. Using these genes for genetic network analysis, we obtained three genetic networks. Moreover, the transcripts of heat-shock protein, including Hsp 40 and Hsp 90, were significantly altered in response to thermal stress.

Conclusions: This study provides a broader understanding of molecular mechanisms underlying stress response in chickens and discovery of novel genes that are regulated in a specific thermal-stress manner.

No MeSH data available.


Related in: MedlinePlus

Heat map. The X-axis is sample expression pattern in different treatment group. The first four samples are heat stress (HS) treatment. The middle four samples are temperature recovery (TR) treatment after heat stress. The last four samples are control group. The Y-axis is the gene clusters across HS, TR, and CL treatment.
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Fig4: Heat map. The X-axis is sample expression pattern in different treatment group. The first four samples are heat stress (HS) treatment. The middle four samples are temperature recovery (TR) treatment after heat stress. The last four samples are control group. The Y-axis is the gene clusters across HS, TR, and CL treatment.

Mentions: Principal component analysis (PCA) was used to classify the broilers based on their gene expression patterns in one-way analysis of variance (ANOVA) with P < 0.05 and FC > 1.5 among three groups (12 samples). The HS group had a transcription profile different from those of the CL and TR groups, proving that heat stress reprograms gene expression in broiler hypothalamus and has a unique expression pattern. Using PCA to observe the clustering of samples, we found that gene expression of HS group showed the largest variation, with the four samples distributing over a wide range. One sample of HS treatment was located in TR treatment, which may be because that bird responded to heat stress very quickly and had its own method for protecting itself. The four CL samples were highly homogenous, while the four TR chickens exhibited proximal patterns distributed similarly to the CL samples (Figure 3). Moreover, a heat map was also developed to investigate the expression pattern and gene clusters (Figure 4). The heat map result is consistent with the PCA result in terms of sample expression pattern. The gene clusters were also subjected to further analysis similar to the gene network analysis (IPA).Figure 3


Transcriptome responses to heat stress in hypothalamus of a meat-type chicken.

Sun H, Jiang R, Xu S, Zhang Z, Xu G, Zheng J, Qu L - J Anim Sci Biotechnol (2015)

Heat map. The X-axis is sample expression pattern in different treatment group. The first four samples are heat stress (HS) treatment. The middle four samples are temperature recovery (TR) treatment after heat stress. The last four samples are control group. The Y-axis is the gene clusters across HS, TR, and CL treatment.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4359534&req=5

Fig4: Heat map. The X-axis is sample expression pattern in different treatment group. The first four samples are heat stress (HS) treatment. The middle four samples are temperature recovery (TR) treatment after heat stress. The last four samples are control group. The Y-axis is the gene clusters across HS, TR, and CL treatment.
Mentions: Principal component analysis (PCA) was used to classify the broilers based on their gene expression patterns in one-way analysis of variance (ANOVA) with P < 0.05 and FC > 1.5 among three groups (12 samples). The HS group had a transcription profile different from those of the CL and TR groups, proving that heat stress reprograms gene expression in broiler hypothalamus and has a unique expression pattern. Using PCA to observe the clustering of samples, we found that gene expression of HS group showed the largest variation, with the four samples distributing over a wide range. One sample of HS treatment was located in TR treatment, which may be because that bird responded to heat stress very quickly and had its own method for protecting itself. The four CL samples were highly homogenous, while the four TR chickens exhibited proximal patterns distributed similarly to the CL samples (Figure 3). Moreover, a heat map was also developed to investigate the expression pattern and gene clusters (Figure 4). The heat map result is consistent with the PCA result in terms of sample expression pattern. The gene clusters were also subjected to further analysis similar to the gene network analysis (IPA).Figure 3

Bottom Line: Consistent expression results were found for 11 selected genes by quantitative real-time PCR.Thirty-eight interesting differential expression genes were found from GO term annotation and those genes were related to meat quality, growth, and crucial enzymes.Moreover, the transcripts of heat-shock protein, including Hsp 40 and Hsp 90, were significantly altered in response to thermal stress.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, 100193 China.

ABSTRACT

Background: Heat stress has resulted in great losses in poultry production. To address this issue, we systematically analyzed chicken hypothalamus transcriptome responses to thermal stress using a 44 k chicken Agilent microarray.

Methods: Hypothalamus samples were collected from a control group reared at 25°C, a heat-stress group treated at 34°C for 24 h, and a temperature-recovery group reared at 25°C for 24 h following a heat-stress treatment. We compared the expression profiles between each pair of the three groups using microarray data.

Results: A total of 1,967 probe sets were found to be differentially expressed in the three comparisons with P < 0.05 and a fold change (FC) higher than 1.5, and the genes were mainly involved in self-regulation and compensation required to maintain homeostasis. Consistent expression results were found for 11 selected genes by quantitative real-time PCR. Thirty-eight interesting differential expression genes were found from GO term annotation and those genes were related to meat quality, growth, and crucial enzymes. Using these genes for genetic network analysis, we obtained three genetic networks. Moreover, the transcripts of heat-shock protein, including Hsp 40 and Hsp 90, were significantly altered in response to thermal stress.

Conclusions: This study provides a broader understanding of molecular mechanisms underlying stress response in chickens and discovery of novel genes that are regulated in a specific thermal-stress manner.

No MeSH data available.


Related in: MedlinePlus