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Transcriptome responses to heat stress in hypothalamus of a meat-type chicken.

Sun H, Jiang R, Xu S, Zhang Z, Xu G, Zheng J, Qu L - J Anim Sci Biotechnol (2015)

Bottom Line: Consistent expression results were found for 11 selected genes by quantitative real-time PCR.Thirty-eight interesting differential expression genes were found from GO term annotation and those genes were related to meat quality, growth, and crucial enzymes.Moreover, the transcripts of heat-shock protein, including Hsp 40 and Hsp 90, were significantly altered in response to thermal stress.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, 100193 China.

ABSTRACT

Background: Heat stress has resulted in great losses in poultry production. To address this issue, we systematically analyzed chicken hypothalamus transcriptome responses to thermal stress using a 44 k chicken Agilent microarray.

Methods: Hypothalamus samples were collected from a control group reared at 25°C, a heat-stress group treated at 34°C for 24 h, and a temperature-recovery group reared at 25°C for 24 h following a heat-stress treatment. We compared the expression profiles between each pair of the three groups using microarray data.

Results: A total of 1,967 probe sets were found to be differentially expressed in the three comparisons with P < 0.05 and a fold change (FC) higher than 1.5, and the genes were mainly involved in self-regulation and compensation required to maintain homeostasis. Consistent expression results were found for 11 selected genes by quantitative real-time PCR. Thirty-eight interesting differential expression genes were found from GO term annotation and those genes were related to meat quality, growth, and crucial enzymes. Using these genes for genetic network analysis, we obtained three genetic networks. Moreover, the transcripts of heat-shock protein, including Hsp 40 and Hsp 90, were significantly altered in response to thermal stress.

Conclusions: This study provides a broader understanding of molecular mechanisms underlying stress response in chickens and discovery of novel genes that are regulated in a specific thermal-stress manner.

No MeSH data available.


Related in: MedlinePlus

The number of differentially expressed genes in three different comparison groups. Note: FDR < 0.05 and fold change ≥ 1.5; HS: heat stress group; CL: control group; TR: temperature recovery group.
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Fig1: The number of differentially expressed genes in three different comparison groups. Note: FDR < 0.05 and fold change ≥ 1.5; HS: heat stress group; CL: control group; TR: temperature recovery group.

Mentions: A comparative analysis was performed by comparing gene expression profiling between each pair of the three groups CL, HS, and TR. Generally, more DE genes were present in the comparison of HS vs. CL and HS vs. TR compared to that of TR vs. CL. In summary, there were 1,239, 846, and 9 probe sets DE in HS vs. CL, HS vs. TR, and TR vs. CL, respectively. These results showed that the TR group is more similar to the CL group. Moreover, 638 of 1,239 probe sets, 493 of 846 probe sets and 5 of 9 probe sets are up-regulated in HS vs. CL, HS vs. TR, and TR vs. CL, respectively (Figure 1). The FC range was 1.5 to 25 in HS vs. CL, 1.5 to 36 in HS vs. TR, and 1.5 to 16 in TR vs. CL. In addition, the pattern of the total 1,743 differential expression probe sets is shown in Figure 2. There were 890, 499, and 3 probe sets uniquely DE in HS vs. CL, HS vs. TR, and TR vs. CL, respectively. Contrasts HS vs. CL and HS vs. TR shared 345 DE genes (Figure 2). All the microarray data have been deposited in the GEO repository (GSE37400, NCBI tracking system #16562670).Figure 1


Transcriptome responses to heat stress in hypothalamus of a meat-type chicken.

Sun H, Jiang R, Xu S, Zhang Z, Xu G, Zheng J, Qu L - J Anim Sci Biotechnol (2015)

The number of differentially expressed genes in three different comparison groups. Note: FDR < 0.05 and fold change ≥ 1.5; HS: heat stress group; CL: control group; TR: temperature recovery group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4359534&req=5

Fig1: The number of differentially expressed genes in three different comparison groups. Note: FDR < 0.05 and fold change ≥ 1.5; HS: heat stress group; CL: control group; TR: temperature recovery group.
Mentions: A comparative analysis was performed by comparing gene expression profiling between each pair of the three groups CL, HS, and TR. Generally, more DE genes were present in the comparison of HS vs. CL and HS vs. TR compared to that of TR vs. CL. In summary, there were 1,239, 846, and 9 probe sets DE in HS vs. CL, HS vs. TR, and TR vs. CL, respectively. These results showed that the TR group is more similar to the CL group. Moreover, 638 of 1,239 probe sets, 493 of 846 probe sets and 5 of 9 probe sets are up-regulated in HS vs. CL, HS vs. TR, and TR vs. CL, respectively (Figure 1). The FC range was 1.5 to 25 in HS vs. CL, 1.5 to 36 in HS vs. TR, and 1.5 to 16 in TR vs. CL. In addition, the pattern of the total 1,743 differential expression probe sets is shown in Figure 2. There were 890, 499, and 3 probe sets uniquely DE in HS vs. CL, HS vs. TR, and TR vs. CL, respectively. Contrasts HS vs. CL and HS vs. TR shared 345 DE genes (Figure 2). All the microarray data have been deposited in the GEO repository (GSE37400, NCBI tracking system #16562670).Figure 1

Bottom Line: Consistent expression results were found for 11 selected genes by quantitative real-time PCR.Thirty-eight interesting differential expression genes were found from GO term annotation and those genes were related to meat quality, growth, and crucial enzymes.Moreover, the transcripts of heat-shock protein, including Hsp 40 and Hsp 90, were significantly altered in response to thermal stress.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, 100193 China.

ABSTRACT

Background: Heat stress has resulted in great losses in poultry production. To address this issue, we systematically analyzed chicken hypothalamus transcriptome responses to thermal stress using a 44 k chicken Agilent microarray.

Methods: Hypothalamus samples were collected from a control group reared at 25°C, a heat-stress group treated at 34°C for 24 h, and a temperature-recovery group reared at 25°C for 24 h following a heat-stress treatment. We compared the expression profiles between each pair of the three groups using microarray data.

Results: A total of 1,967 probe sets were found to be differentially expressed in the three comparisons with P < 0.05 and a fold change (FC) higher than 1.5, and the genes were mainly involved in self-regulation and compensation required to maintain homeostasis. Consistent expression results were found for 11 selected genes by quantitative real-time PCR. Thirty-eight interesting differential expression genes were found from GO term annotation and those genes were related to meat quality, growth, and crucial enzymes. Using these genes for genetic network analysis, we obtained three genetic networks. Moreover, the transcripts of heat-shock protein, including Hsp 40 and Hsp 90, were significantly altered in response to thermal stress.

Conclusions: This study provides a broader understanding of molecular mechanisms underlying stress response in chickens and discovery of novel genes that are regulated in a specific thermal-stress manner.

No MeSH data available.


Related in: MedlinePlus