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Kidney injury molecule-1 expression in human kidney transplants with interstitial fibrosis and tubular atrophy.

Nogare AL, Veronese FV, Carpio VN, Montenegro RM, Pedroso JA, Pegas KL, Gonçalves LF, Manfro RC - BMC Nephrol (2015)

Bottom Line: In the urine cells KIM-1 mRNA was also increased in patients with IF/TA compared with patients with acute CIN (P <0.05).Significant correlations were found between KIM-1 protein and mRNA levels in tissue, between mRNA expressions in tissue and urine and between protein tissue expression and gene expression in the urine.Urinary KIM-1 mRNA may become a useful non-invasive biomarker of the injuries that can trigger intra-graft fibrotic processes, such as interstitial fibrosis and tubular atrophy.

View Article: PubMed Central - PubMed

Affiliation: Post-Graduate Medical Sciences Program, School of Medicine, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil. alnogare@gmail.com.

ABSTRACT

Background: Kidney injury molecule-1 (KIM-1) is expressed in tubular epithelial cells after injury and may have a role in the development of renal graft fibrosis. In this study we evaluated the molecular and protein expressions of KIM-1 in dysfunctional allografts and also mRNA KIM-1 expression in urine as potential biomarkers of graft fibrosis.

Methods: Protein and mRNA levels in renal tissue and urinary sediment cells of 69 kidney transplant recipients that undertook for-cause graft biopsies were evaluated by immunohistochemistry and real-time polymerase chain reaction. The histopathology was classified according to the 2007 Banff schema.

Results: KIM-1 protein expression was increased in biopsies with interstitial fibrosis and tubular atrophy (IF/TA) compared with biopsies showing acute calcineurin inhibitor nephrotoxicity (CIN) (P <0.05). Kidney tissue KIM-1 mRNA signaling (in) was increased in biopsies with IF/TA compared with all other groups (P <0.05). In the urine cells KIM-1 mRNA was also increased in patients with IF/TA compared with patients with acute CIN (P <0.05). Significant correlations were found between KIM-1 protein and mRNA levels in tissue, between mRNA expressions in tissue and urine and between protein tissue expression and gene expression in the urine.

Conclusions: KIM-1 seems to be a marker of kidney graft fibrosis. Urinary KIM-1 mRNA may become a useful non-invasive biomarker of the injuries that can trigger intra-graft fibrotic processes, such as interstitial fibrosis and tubular atrophy.

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Related in: MedlinePlus

Immunohistochemical staining of KIM-1 in non-atrophic proximal tubules of kidney transplant for-cause biopsies according to staining intensity. Slide A: interstitial fibrosis and tubular atrophy, 3+; Slide B: acute tubular necrosis, 2+; Slide C; acute rejection, 1+; Slide D: calcineurin inhibitor nephrotoxicity, very weak staining (original magnification x 400 in A-D).
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Fig2: Immunohistochemical staining of KIM-1 in non-atrophic proximal tubules of kidney transplant for-cause biopsies according to staining intensity. Slide A: interstitial fibrosis and tubular atrophy, 3+; Slide B: acute tubular necrosis, 2+; Slide C; acute rejection, 1+; Slide D: calcineurin inhibitor nephrotoxicity, very weak staining (original magnification x 400 in A-D).

Mentions: Immunohistochemistry slides showing KIM-1 protein expression in patients with post-transplant IF/TA, ATN, AR and CIN are shown in the four panels of Figure 2.Figure 2


Kidney injury molecule-1 expression in human kidney transplants with interstitial fibrosis and tubular atrophy.

Nogare AL, Veronese FV, Carpio VN, Montenegro RM, Pedroso JA, Pegas KL, Gonçalves LF, Manfro RC - BMC Nephrol (2015)

Immunohistochemical staining of KIM-1 in non-atrophic proximal tubules of kidney transplant for-cause biopsies according to staining intensity. Slide A: interstitial fibrosis and tubular atrophy, 3+; Slide B: acute tubular necrosis, 2+; Slide C; acute rejection, 1+; Slide D: calcineurin inhibitor nephrotoxicity, very weak staining (original magnification x 400 in A-D).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4359521&req=5

Fig2: Immunohistochemical staining of KIM-1 in non-atrophic proximal tubules of kidney transplant for-cause biopsies according to staining intensity. Slide A: interstitial fibrosis and tubular atrophy, 3+; Slide B: acute tubular necrosis, 2+; Slide C; acute rejection, 1+; Slide D: calcineurin inhibitor nephrotoxicity, very weak staining (original magnification x 400 in A-D).
Mentions: Immunohistochemistry slides showing KIM-1 protein expression in patients with post-transplant IF/TA, ATN, AR and CIN are shown in the four panels of Figure 2.Figure 2

Bottom Line: In the urine cells KIM-1 mRNA was also increased in patients with IF/TA compared with patients with acute CIN (P <0.05).Significant correlations were found between KIM-1 protein and mRNA levels in tissue, between mRNA expressions in tissue and urine and between protein tissue expression and gene expression in the urine.Urinary KIM-1 mRNA may become a useful non-invasive biomarker of the injuries that can trigger intra-graft fibrotic processes, such as interstitial fibrosis and tubular atrophy.

View Article: PubMed Central - PubMed

Affiliation: Post-Graduate Medical Sciences Program, School of Medicine, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil. alnogare@gmail.com.

ABSTRACT

Background: Kidney injury molecule-1 (KIM-1) is expressed in tubular epithelial cells after injury and may have a role in the development of renal graft fibrosis. In this study we evaluated the molecular and protein expressions of KIM-1 in dysfunctional allografts and also mRNA KIM-1 expression in urine as potential biomarkers of graft fibrosis.

Methods: Protein and mRNA levels in renal tissue and urinary sediment cells of 69 kidney transplant recipients that undertook for-cause graft biopsies were evaluated by immunohistochemistry and real-time polymerase chain reaction. The histopathology was classified according to the 2007 Banff schema.

Results: KIM-1 protein expression was increased in biopsies with interstitial fibrosis and tubular atrophy (IF/TA) compared with biopsies showing acute calcineurin inhibitor nephrotoxicity (CIN) (P <0.05). Kidney tissue KIM-1 mRNA signaling (in) was increased in biopsies with IF/TA compared with all other groups (P <0.05). In the urine cells KIM-1 mRNA was also increased in patients with IF/TA compared with patients with acute CIN (P <0.05). Significant correlations were found between KIM-1 protein and mRNA levels in tissue, between mRNA expressions in tissue and urine and between protein tissue expression and gene expression in the urine.

Conclusions: KIM-1 seems to be a marker of kidney graft fibrosis. Urinary KIM-1 mRNA may become a useful non-invasive biomarker of the injuries that can trigger intra-graft fibrotic processes, such as interstitial fibrosis and tubular atrophy.

Show MeSH
Related in: MedlinePlus