Miniaturized plate readers for low-cost, high-throughput phenotypic screening.
Bottom Line: The device contains no moving parts, allowing an entire 96-well plate to be read several times per second.A wireless communication module allows remote monitoring of multiple devices in real time.These features allow easy assembly of multiple readers to create a scalable, accurate solution for high-throughput phenotypic screening.
Affiliation: Department of Biomedical Engineering, University of Virginia, Charlottesville, VA, USA Biology Department, Boston College, Chestnut Hill, MA, USA.Show MeSH
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Mentions: The miniaturized reader can be operated in a “standalone” configuration (Fig. 4A). The device can be powered with a single 9 V battery for several days. We included a ZigBee wireless communication radio (ZigBee, San Ramon, CA, http://www.digi.com/xbee) to transmit data to a remote server for real-time data storage and visualization (Fig. 4B). The server can track and configure multiple devices via the internet (Fig. 4C). We have operated up to 16 devices simultaneously. Many more devices can be connected, with the total number limited only by the available wireless bandwidth. We have also integrated an on-board single-chip computer (the Raspberry Pi: Raspberry Pi Foundation, Caldecott, UK, http://www.raspberrypi.org) to provide data storage and visualization without an external server (Fig. 4D). The on-board computer allows use of the reader in remote field locations without access to additional computers or wireless networks.
Affiliation: Department of Biomedical Engineering, University of Virginia, Charlottesville, VA, USA Biology Department, Boston College, Chestnut Hill, MA, USA.