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Miniaturized plate readers for low-cost, high-throughput phenotypic screening.

Jensen PA, Dougherty BV, Moutinho TJ, Papin JA - J Lab Autom (2014)

Bottom Line: The device contains no moving parts, allowing an entire 96-well plate to be read several times per second.A wireless communication module allows remote monitoring of multiple devices in real time.These features allow easy assembly of multiple readers to create a scalable, accurate solution for high-throughput phenotypic screening.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, University of Virginia, Charlottesville, VA, USA Biology Department, Boston College, Chestnut Hill, MA, USA.

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Related in: MedlinePlus

Multiple configurations for the miniaturized plate reader system. (A) Standalone reader with data stored on a Secure Digital (SD) memory card. (B) Reader transmitting data to a database server for storage and processing. (C) Multiple readers as part of a high-throughput screening system. (D) Reader with on-board computer. The computer provides a Wi-Fi hotspot to visualize data through the internet.
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fig4-2211068214555414: Multiple configurations for the miniaturized plate reader system. (A) Standalone reader with data stored on a Secure Digital (SD) memory card. (B) Reader transmitting data to a database server for storage and processing. (C) Multiple readers as part of a high-throughput screening system. (D) Reader with on-board computer. The computer provides a Wi-Fi hotspot to visualize data through the internet.

Mentions: The miniaturized reader can be operated in a “standalone” configuration (Fig. 4A). The device can be powered with a single 9 V battery for several days. We included a ZigBee wireless communication radio (ZigBee, San Ramon, CA, http://www.digi.com/xbee) to transmit data to a remote server for real-time data storage and visualization (Fig. 4B). The server can track and configure multiple devices via the internet (Fig. 4C). We have operated up to 16 devices simultaneously. Many more devices can be connected, with the total number limited only by the available wireless bandwidth. We have also integrated an on-board single-chip computer (the Raspberry Pi: Raspberry Pi Foundation, Caldecott, UK, http://www.raspberrypi.org) to provide data storage and visualization without an external server (Fig. 4D). The on-board computer allows use of the reader in remote field locations without access to additional computers or wireless networks.


Miniaturized plate readers for low-cost, high-throughput phenotypic screening.

Jensen PA, Dougherty BV, Moutinho TJ, Papin JA - J Lab Autom (2014)

Multiple configurations for the miniaturized plate reader system. (A) Standalone reader with data stored on a Secure Digital (SD) memory card. (B) Reader transmitting data to a database server for storage and processing. (C) Multiple readers as part of a high-throughput screening system. (D) Reader with on-board computer. The computer provides a Wi-Fi hotspot to visualize data through the internet.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2 - License 3
Show All Figures
getmorefigures.php?uid=PMC4359207&req=5

fig4-2211068214555414: Multiple configurations for the miniaturized plate reader system. (A) Standalone reader with data stored on a Secure Digital (SD) memory card. (B) Reader transmitting data to a database server for storage and processing. (C) Multiple readers as part of a high-throughput screening system. (D) Reader with on-board computer. The computer provides a Wi-Fi hotspot to visualize data through the internet.
Mentions: The miniaturized reader can be operated in a “standalone” configuration (Fig. 4A). The device can be powered with a single 9 V battery for several days. We included a ZigBee wireless communication radio (ZigBee, San Ramon, CA, http://www.digi.com/xbee) to transmit data to a remote server for real-time data storage and visualization (Fig. 4B). The server can track and configure multiple devices via the internet (Fig. 4C). We have operated up to 16 devices simultaneously. Many more devices can be connected, with the total number limited only by the available wireless bandwidth. We have also integrated an on-board single-chip computer (the Raspberry Pi: Raspberry Pi Foundation, Caldecott, UK, http://www.raspberrypi.org) to provide data storage and visualization without an external server (Fig. 4D). The on-board computer allows use of the reader in remote field locations without access to additional computers or wireless networks.

Bottom Line: The device contains no moving parts, allowing an entire 96-well plate to be read several times per second.A wireless communication module allows remote monitoring of multiple devices in real time.These features allow easy assembly of multiple readers to create a scalable, accurate solution for high-throughput phenotypic screening.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, University of Virginia, Charlottesville, VA, USA Biology Department, Boston College, Chestnut Hill, MA, USA.

Show MeSH
Related in: MedlinePlus