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Miniaturized plate readers for low-cost, high-throughput phenotypic screening.

Jensen PA, Dougherty BV, Moutinho TJ, Papin JA - J Lab Autom (2014)

Bottom Line: The device contains no moving parts, allowing an entire 96-well plate to be read several times per second.A wireless communication module allows remote monitoring of multiple devices in real time.These features allow easy assembly of multiple readers to create a scalable, accurate solution for high-throughput phenotypic screening.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, University of Virginia, Charlottesville, VA, USA Biology Department, Boston College, Chestnut Hill, MA, USA.

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Related in: MedlinePlus

(A) Performance comparison between our miniaturized plate reader (vertical) and a commercial system, the Tecan Infinite Pro 200 (horizontal). The bacteria Escherichia coli K12 and Pseudomonas aeruginosa PA14 were grown in multiple conditions. Growth rates were normalized to E. coli grown in LB with supplemental glucose. Each point represents a biological replicate. Vertical and horizontal bars indicate the standard deviation from 24 technical replicates. (B) Comparison between miniaturized plate reader (vertical) and batch cultures grown in 500 ml flasks (horizontal). Growth rates were normalized to E. coli grown in LB. Each point represents a biological replicate. (C) Shaking does not affect device performance. Comparison between growth curve of P. aeruginosa in LB, with (blue) and without (red) shaking at 100 rpm. Readings were collected every 120 s.
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fig3-2211068214555414: (A) Performance comparison between our miniaturized plate reader (vertical) and a commercial system, the Tecan Infinite Pro 200 (horizontal). The bacteria Escherichia coli K12 and Pseudomonas aeruginosa PA14 were grown in multiple conditions. Growth rates were normalized to E. coli grown in LB with supplemental glucose. Each point represents a biological replicate. Vertical and horizontal bars indicate the standard deviation from 24 technical replicates. (B) Comparison between miniaturized plate reader (vertical) and batch cultures grown in 500 ml flasks (horizontal). Growth rates were normalized to E. coli grown in LB. Each point represents a biological replicate. (C) Shaking does not affect device performance. Comparison between growth curve of P. aeruginosa in LB, with (blue) and without (red) shaking at 100 rpm. Readings were collected every 120 s.

Mentions: We tested our miniaturized plate reader against a commercial plate reader using a variety of conditions. Cultures of the bacteria E. coli K12 and P. aeruginosa PA14 were grown in multiple media: a rich base (LB) with supplemental glucose or lactate; a minimal base (M9) with supplemental arginine, glucose, or glycerol; and SCFM, a media designed to recreate the in vivo metabolic environment of the cystic fibrotic lung.7 We compared the exponential phase growth rates between our device and either a commercial incubating plate reader—the Tecan Infinite Pro 200 (Fig. 3A)—or batch cultures grown in 500 ml flasks (Fig. 3B). There was excellent agreement among the growth rates measured by each device.


Miniaturized plate readers for low-cost, high-throughput phenotypic screening.

Jensen PA, Dougherty BV, Moutinho TJ, Papin JA - J Lab Autom (2014)

(A) Performance comparison between our miniaturized plate reader (vertical) and a commercial system, the Tecan Infinite Pro 200 (horizontal). The bacteria Escherichia coli K12 and Pseudomonas aeruginosa PA14 were grown in multiple conditions. Growth rates were normalized to E. coli grown in LB with supplemental glucose. Each point represents a biological replicate. Vertical and horizontal bars indicate the standard deviation from 24 technical replicates. (B) Comparison between miniaturized plate reader (vertical) and batch cultures grown in 500 ml flasks (horizontal). Growth rates were normalized to E. coli grown in LB. Each point represents a biological replicate. (C) Shaking does not affect device performance. Comparison between growth curve of P. aeruginosa in LB, with (blue) and without (red) shaking at 100 rpm. Readings were collected every 120 s.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2 - License 3
Show All Figures
getmorefigures.php?uid=PMC4359207&req=5

fig3-2211068214555414: (A) Performance comparison between our miniaturized plate reader (vertical) and a commercial system, the Tecan Infinite Pro 200 (horizontal). The bacteria Escherichia coli K12 and Pseudomonas aeruginosa PA14 were grown in multiple conditions. Growth rates were normalized to E. coli grown in LB with supplemental glucose. Each point represents a biological replicate. Vertical and horizontal bars indicate the standard deviation from 24 technical replicates. (B) Comparison between miniaturized plate reader (vertical) and batch cultures grown in 500 ml flasks (horizontal). Growth rates were normalized to E. coli grown in LB. Each point represents a biological replicate. (C) Shaking does not affect device performance. Comparison between growth curve of P. aeruginosa in LB, with (blue) and without (red) shaking at 100 rpm. Readings were collected every 120 s.
Mentions: We tested our miniaturized plate reader against a commercial plate reader using a variety of conditions. Cultures of the bacteria E. coli K12 and P. aeruginosa PA14 were grown in multiple media: a rich base (LB) with supplemental glucose or lactate; a minimal base (M9) with supplemental arginine, glucose, or glycerol; and SCFM, a media designed to recreate the in vivo metabolic environment of the cystic fibrotic lung.7 We compared the exponential phase growth rates between our device and either a commercial incubating plate reader—the Tecan Infinite Pro 200 (Fig. 3A)—or batch cultures grown in 500 ml flasks (Fig. 3B). There was excellent agreement among the growth rates measured by each device.

Bottom Line: The device contains no moving parts, allowing an entire 96-well plate to be read several times per second.A wireless communication module allows remote monitoring of multiple devices in real time.These features allow easy assembly of multiple readers to create a scalable, accurate solution for high-throughput phenotypic screening.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, University of Virginia, Charlottesville, VA, USA Biology Department, Boston College, Chestnut Hill, MA, USA.

Show MeSH
Related in: MedlinePlus