Miniaturized plate readers for low-cost, high-throughput phenotypic screening.
Bottom Line: The device contains no moving parts, allowing an entire 96-well plate to be read several times per second.A wireless communication module allows remote monitoring of multiple devices in real time.These features allow easy assembly of multiple readers to create a scalable, accurate solution for high-throughput phenotypic screening.
Affiliation: Department of Biomedical Engineering, University of Virginia, Charlottesville, VA, USA Biology Department, Boston College, Chestnut Hill, MA, USA.Show MeSH
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Mentions: We tested our miniaturized plate reader against a commercial plate reader using a variety of conditions. Cultures of the bacteria E. coli K12 and P. aeruginosa PA14 were grown in multiple media: a rich base (LB) with supplemental glucose or lactate; a minimal base (M9) with supplemental arginine, glucose, or glycerol; and SCFM, a media designed to recreate the in vivo metabolic environment of the cystic fibrotic lung.7 We compared the exponential phase growth rates between our device and either a commercial incubating plate reader—the Tecan Infinite Pro 200 (Fig. 3A)—or batch cultures grown in 500 ml flasks (Fig. 3B). There was excellent agreement among the growth rates measured by each device.
Affiliation: Department of Biomedical Engineering, University of Virginia, Charlottesville, VA, USA Biology Department, Boston College, Chestnut Hill, MA, USA.