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Miniaturized plate readers for low-cost, high-throughput phenotypic screening.

Jensen PA, Dougherty BV, Moutinho TJ, Papin JA - J Lab Autom (2014)

Bottom Line: The device contains no moving parts, allowing an entire 96-well plate to be read several times per second.A wireless communication module allows remote monitoring of multiple devices in real time.These features allow easy assembly of multiple readers to create a scalable, accurate solution for high-throughput phenotypic screening.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, University of Virginia, Charlottesville, VA, USA Biology Department, Boston College, Chestnut Hill, MA, USA.

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Related in: MedlinePlus

Design of a miniaturized multiwell optical density (OD) reader. (A) Schematic for OD sensor. An infrared light-emitting diode (LED) emits light at 870 nm, which is received by a phototransistor (PT). The light results in a proportional drop in voltage across the 100k ohm resistor, which is read at VOUT (output voltage). (B) Sensor output varies linearly throughout a range of OD. Readings for 96 wells on a single plate shown at 9 dilutions of a bacterial suspension. (C) A multiplexed grid for reading individual wells. Each of the 96 LEDs can be connected to the circuit independently by switching 2 analog multiplexers. One multiplexer connects all anodes in a single column, and the second connects all cathodes in a single row. The red line indicates an active connection through the LED corresponding to well D8. The same board layout can be used to connect the PTs above the multiwell plate.
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fig1-2211068214555414: Design of a miniaturized multiwell optical density (OD) reader. (A) Schematic for OD sensor. An infrared light-emitting diode (LED) emits light at 870 nm, which is received by a phototransistor (PT). The light results in a proportional drop in voltage across the 100k ohm resistor, which is read at VOUT (output voltage). (B) Sensor output varies linearly throughout a range of OD. Readings for 96 wells on a single plate shown at 9 dilutions of a bacterial suspension. (C) A multiplexed grid for reading individual wells. Each of the 96 LEDs can be connected to the circuit independently by switching 2 analog multiplexers. One multiplexer connects all anodes in a single column, and the second connects all cathodes in a single row. The red line indicates an active connection through the LED corresponding to well D8. The same board layout can be used to connect the PTs above the multiwell plate.

Mentions: A single circuit for measuring OD is shown in Figure 1A. An LED produces a consistent light source at a fixed wavelength. The light travels vertically through the well of a microtiter plate, and transmitted light falls on a PT. We observed a linear response throughout a physiologically reasonable OD range (Fig. 1B).


Miniaturized plate readers for low-cost, high-throughput phenotypic screening.

Jensen PA, Dougherty BV, Moutinho TJ, Papin JA - J Lab Autom (2014)

Design of a miniaturized multiwell optical density (OD) reader. (A) Schematic for OD sensor. An infrared light-emitting diode (LED) emits light at 870 nm, which is received by a phototransistor (PT). The light results in a proportional drop in voltage across the 100k ohm resistor, which is read at VOUT (output voltage). (B) Sensor output varies linearly throughout a range of OD. Readings for 96 wells on a single plate shown at 9 dilutions of a bacterial suspension. (C) A multiplexed grid for reading individual wells. Each of the 96 LEDs can be connected to the circuit independently by switching 2 analog multiplexers. One multiplexer connects all anodes in a single column, and the second connects all cathodes in a single row. The red line indicates an active connection through the LED corresponding to well D8. The same board layout can be used to connect the PTs above the multiwell plate.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2 - License 3
Show All Figures
getmorefigures.php?uid=PMC4359207&req=5

fig1-2211068214555414: Design of a miniaturized multiwell optical density (OD) reader. (A) Schematic for OD sensor. An infrared light-emitting diode (LED) emits light at 870 nm, which is received by a phototransistor (PT). The light results in a proportional drop in voltage across the 100k ohm resistor, which is read at VOUT (output voltage). (B) Sensor output varies linearly throughout a range of OD. Readings for 96 wells on a single plate shown at 9 dilutions of a bacterial suspension. (C) A multiplexed grid for reading individual wells. Each of the 96 LEDs can be connected to the circuit independently by switching 2 analog multiplexers. One multiplexer connects all anodes in a single column, and the second connects all cathodes in a single row. The red line indicates an active connection through the LED corresponding to well D8. The same board layout can be used to connect the PTs above the multiwell plate.
Mentions: A single circuit for measuring OD is shown in Figure 1A. An LED produces a consistent light source at a fixed wavelength. The light travels vertically through the well of a microtiter plate, and transmitted light falls on a PT. We observed a linear response throughout a physiologically reasonable OD range (Fig. 1B).

Bottom Line: The device contains no moving parts, allowing an entire 96-well plate to be read several times per second.A wireless communication module allows remote monitoring of multiple devices in real time.These features allow easy assembly of multiple readers to create a scalable, accurate solution for high-throughput phenotypic screening.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, University of Virginia, Charlottesville, VA, USA Biology Department, Boston College, Chestnut Hill, MA, USA.

Show MeSH
Related in: MedlinePlus