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Deep sequencing and ecological characterization of gut microbial communities of diverse bumble bee species.

Lim HC, Chu CC, Seufferheld MJ, Cameron SA - PLoS ONE (2015)

Bottom Line: The most abundant and prevalent (occurring in 92% of the samples) bacterial OTU, based on 16S rRNA sequences, closely matched that of the previously described Betaproteobacteria species Snodgrassella alvi.Bacteria that were first described in bee-related external environments dominated a number of gut bacterial communities, suggesting that they are not strictly dependent on the internal gut environment.Using light and fluorescent microscopy, we demonstrated that the gut bacteria form a biofilm on the internal epithelial surface of the ileum, corroborating results obtained from Apis mellifera.

View Article: PubMed Central - PubMed

Affiliation: Department of Entomology, University of Illinois, Urbana, IL, 61801, United States of America.

ABSTRACT
Gut bacterial communities of bumble bees are correlated with defense against pathogens. Further understanding this host-microbe association is vitally important as bumble bees are currently experiencing global population declines, potentially due in part to emergent diseases. In this study, we used pyrosequencing and community fingerprinting (ARISA) to characterize the gut microbial communities of nine bumble species from across the Bombus phylogeny. Overall, we delimited 74 bacterial taxa (operational taxonomic units or OTUs) belonging to Betaproteobacteria, Gammaproteobacteria, Bacilli, Actinobacteria, Flavobacteria and Alphaproteobacteria. Each bacterial community was taxonomically simple, containing an average of 1.9 common (relative abundance per sample > 5%) bacterial OTUs. The most abundant and prevalent (occurring in 92% of the samples) bacterial OTU, based on 16S rRNA sequences, closely matched that of the previously described Betaproteobacteria species Snodgrassella alvi. Bacteria that were first described in bee-related external environments dominated a number of gut bacterial communities, suggesting that they are not strictly dependent on the internal gut environment. The ARISA data showed a correlation between bacterial community structures and the geographic locations where the bees were sampled, suggesting that at least a subset of the bacterial species may be transmitted environmentally. Using light and fluorescent microscopy, we demonstrated that the gut bacteria form a biofilm on the internal epithelial surface of the ileum, corroborating results obtained from Apis mellifera.

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Three-dimensional (DIM1–3) nMDS ordination of ARISA profiles of different bumble bee gut DNA samples.Corresponding bumble bee species and populations (collection sites) are illustrated by the colors and shapes of the markers on the right. Abbreviations: B.FR = B. fraternus; B.IM = B. impatiens; B.PE = B. pensylvanicus; B.TE = B. terricola; B.TRS = B. terrestris. Abbreviations following species names represent different collection sites, sorted by states in the US (FL, GA, MI, MN, NC, SC, WI, and WV), or across countries (ARG and FRA). ARG: Argentina, FRA: France.
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pone.0118566.g005: Three-dimensional (DIM1–3) nMDS ordination of ARISA profiles of different bumble bee gut DNA samples.Corresponding bumble bee species and populations (collection sites) are illustrated by the colors and shapes of the markers on the right. Abbreviations: B.FR = B. fraternus; B.IM = B. impatiens; B.PE = B. pensylvanicus; B.TE = B. terricola; B.TRS = B. terrestris. Abbreviations following species names represent different collection sites, sorted by states in the US (FL, GA, MI, MN, NC, SC, WI, and WV), or across countries (ARG and FRA). ARG: Argentina, FRA: France.

Mentions: After noise-filtering of the ARISA data, we observed an average of 125 fragments per sample. A 3-D plot of the NMDS analysis based on Bray-Curtis dissimilarity measures showed that samples tended to cluster by bumble bee species, and by locations within species (Fig. 5). The plot also showed trends resembling that of the 2-D NMDS plot based on the sequencing data (Fig. 4); microbial communities of B. fraternus were relatively distinct from those of the other four Bombus species analyzed (B. pensylvanicus, B. terricola, B. terrestris, and B. impatiens). A two-way unbalanced PERMANOVA including “species” (fixed effect) and “locality” (random effect nested in species) showed that both factors significantly influenced the gut microbiota structures of the bees examined (Species, P = 0.0001; Locality, P = 0.0001).


Deep sequencing and ecological characterization of gut microbial communities of diverse bumble bee species.

Lim HC, Chu CC, Seufferheld MJ, Cameron SA - PLoS ONE (2015)

Three-dimensional (DIM1–3) nMDS ordination of ARISA profiles of different bumble bee gut DNA samples.Corresponding bumble bee species and populations (collection sites) are illustrated by the colors and shapes of the markers on the right. Abbreviations: B.FR = B. fraternus; B.IM = B. impatiens; B.PE = B. pensylvanicus; B.TE = B. terricola; B.TRS = B. terrestris. Abbreviations following species names represent different collection sites, sorted by states in the US (FL, GA, MI, MN, NC, SC, WI, and WV), or across countries (ARG and FRA). ARG: Argentina, FRA: France.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4359114&req=5

pone.0118566.g005: Three-dimensional (DIM1–3) nMDS ordination of ARISA profiles of different bumble bee gut DNA samples.Corresponding bumble bee species and populations (collection sites) are illustrated by the colors and shapes of the markers on the right. Abbreviations: B.FR = B. fraternus; B.IM = B. impatiens; B.PE = B. pensylvanicus; B.TE = B. terricola; B.TRS = B. terrestris. Abbreviations following species names represent different collection sites, sorted by states in the US (FL, GA, MI, MN, NC, SC, WI, and WV), or across countries (ARG and FRA). ARG: Argentina, FRA: France.
Mentions: After noise-filtering of the ARISA data, we observed an average of 125 fragments per sample. A 3-D plot of the NMDS analysis based on Bray-Curtis dissimilarity measures showed that samples tended to cluster by bumble bee species, and by locations within species (Fig. 5). The plot also showed trends resembling that of the 2-D NMDS plot based on the sequencing data (Fig. 4); microbial communities of B. fraternus were relatively distinct from those of the other four Bombus species analyzed (B. pensylvanicus, B. terricola, B. terrestris, and B. impatiens). A two-way unbalanced PERMANOVA including “species” (fixed effect) and “locality” (random effect nested in species) showed that both factors significantly influenced the gut microbiota structures of the bees examined (Species, P = 0.0001; Locality, P = 0.0001).

Bottom Line: The most abundant and prevalent (occurring in 92% of the samples) bacterial OTU, based on 16S rRNA sequences, closely matched that of the previously described Betaproteobacteria species Snodgrassella alvi.Bacteria that were first described in bee-related external environments dominated a number of gut bacterial communities, suggesting that they are not strictly dependent on the internal gut environment.Using light and fluorescent microscopy, we demonstrated that the gut bacteria form a biofilm on the internal epithelial surface of the ileum, corroborating results obtained from Apis mellifera.

View Article: PubMed Central - PubMed

Affiliation: Department of Entomology, University of Illinois, Urbana, IL, 61801, United States of America.

ABSTRACT
Gut bacterial communities of bumble bees are correlated with defense against pathogens. Further understanding this host-microbe association is vitally important as bumble bees are currently experiencing global population declines, potentially due in part to emergent diseases. In this study, we used pyrosequencing and community fingerprinting (ARISA) to characterize the gut microbial communities of nine bumble species from across the Bombus phylogeny. Overall, we delimited 74 bacterial taxa (operational taxonomic units or OTUs) belonging to Betaproteobacteria, Gammaproteobacteria, Bacilli, Actinobacteria, Flavobacteria and Alphaproteobacteria. Each bacterial community was taxonomically simple, containing an average of 1.9 common (relative abundance per sample > 5%) bacterial OTUs. The most abundant and prevalent (occurring in 92% of the samples) bacterial OTU, based on 16S rRNA sequences, closely matched that of the previously described Betaproteobacteria species Snodgrassella alvi. Bacteria that were first described in bee-related external environments dominated a number of gut bacterial communities, suggesting that they are not strictly dependent on the internal gut environment. The ARISA data showed a correlation between bacterial community structures and the geographic locations where the bees were sampled, suggesting that at least a subset of the bacterial species may be transmitted environmentally. Using light and fluorescent microscopy, we demonstrated that the gut bacteria form a biofilm on the internal epithelial surface of the ileum, corroborating results obtained from Apis mellifera.

Show MeSH
Related in: MedlinePlus