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Deep sequencing and ecological characterization of gut microbial communities of diverse bumble bee species.

Lim HC, Chu CC, Seufferheld MJ, Cameron SA - PLoS ONE (2015)

Bottom Line: The most abundant and prevalent (occurring in 92% of the samples) bacterial OTU, based on 16S rRNA sequences, closely matched that of the previously described Betaproteobacteria species Snodgrassella alvi.Bacteria that were first described in bee-related external environments dominated a number of gut bacterial communities, suggesting that they are not strictly dependent on the internal gut environment.Using light and fluorescent microscopy, we demonstrated that the gut bacteria form a biofilm on the internal epithelial surface of the ileum, corroborating results obtained from Apis mellifera.

View Article: PubMed Central - PubMed

Affiliation: Department of Entomology, University of Illinois, Urbana, IL, 61801, United States of America.

ABSTRACT
Gut bacterial communities of bumble bees are correlated with defense against pathogens. Further understanding this host-microbe association is vitally important as bumble bees are currently experiencing global population declines, potentially due in part to emergent diseases. In this study, we used pyrosequencing and community fingerprinting (ARISA) to characterize the gut microbial communities of nine bumble species from across the Bombus phylogeny. Overall, we delimited 74 bacterial taxa (operational taxonomic units or OTUs) belonging to Betaproteobacteria, Gammaproteobacteria, Bacilli, Actinobacteria, Flavobacteria and Alphaproteobacteria. Each bacterial community was taxonomically simple, containing an average of 1.9 common (relative abundance per sample > 5%) bacterial OTUs. The most abundant and prevalent (occurring in 92% of the samples) bacterial OTU, based on 16S rRNA sequences, closely matched that of the previously described Betaproteobacteria species Snodgrassella alvi. Bacteria that were first described in bee-related external environments dominated a number of gut bacterial communities, suggesting that they are not strictly dependent on the internal gut environment. The ARISA data showed a correlation between bacterial community structures and the geographic locations where the bees were sampled, suggesting that at least a subset of the bacterial species may be transmitted environmentally. Using light and fluorescent microscopy, we demonstrated that the gut bacteria form a biofilm on the internal epithelial surface of the ileum, corroborating results obtained from Apis mellifera.

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Relative abundance (averaged across 13 pyrosequencing samples) of different bacterial classes.The average number of sequences obtained from each pyrosequencing sample was 15,170.
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pone.0118566.g003: Relative abundance (averaged across 13 pyrosequencing samples) of different bacterial classes.The average number of sequences obtained from each pyrosequencing sample was 15,170.

Mentions: Sequencing of all 13 pyrosequencing samples revealed bacteria from only six classes: Actinobacteria, Alphaproteobacteria, Bacilli, Betaproteobacteria, Flavobacteria and Gammaproteobacteria (Table 2). Overall, Betaproteobacteria was the most dominant class, accounting for 52.7 ± 42.2% of the bacterial sequences when abundance was averaged across 13 pyrosequencing samples. This was followed by Bacilli (18.5 ± 36.0%) and Gammaproteobacteria (13.5 ± 24.9%) (Fig. 3, S1 Table). The number of OTUs found in each class varied widely; it ranged from 20 each in Bacilli and Betaproteobacteria, to two in Alphaproteobacteria. Bacterial Orders containing OTUs with high abundance included Neisseriales (Betaproteobacteria), Lactobacillales (Bacilli) and Enterobacteriales (Gammaproteobacteria). At the opposite end of the spectrum, bacterial orders with OTUs with low overall abundance included Burkholderiales (Betaproteobacteria), Oceanospirillales (Gammaproteobacteria) and Bifidobacteriales (Actinobacteria) (Table 2).


Deep sequencing and ecological characterization of gut microbial communities of diverse bumble bee species.

Lim HC, Chu CC, Seufferheld MJ, Cameron SA - PLoS ONE (2015)

Relative abundance (averaged across 13 pyrosequencing samples) of different bacterial classes.The average number of sequences obtained from each pyrosequencing sample was 15,170.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4359114&req=5

pone.0118566.g003: Relative abundance (averaged across 13 pyrosequencing samples) of different bacterial classes.The average number of sequences obtained from each pyrosequencing sample was 15,170.
Mentions: Sequencing of all 13 pyrosequencing samples revealed bacteria from only six classes: Actinobacteria, Alphaproteobacteria, Bacilli, Betaproteobacteria, Flavobacteria and Gammaproteobacteria (Table 2). Overall, Betaproteobacteria was the most dominant class, accounting for 52.7 ± 42.2% of the bacterial sequences when abundance was averaged across 13 pyrosequencing samples. This was followed by Bacilli (18.5 ± 36.0%) and Gammaproteobacteria (13.5 ± 24.9%) (Fig. 3, S1 Table). The number of OTUs found in each class varied widely; it ranged from 20 each in Bacilli and Betaproteobacteria, to two in Alphaproteobacteria. Bacterial Orders containing OTUs with high abundance included Neisseriales (Betaproteobacteria), Lactobacillales (Bacilli) and Enterobacteriales (Gammaproteobacteria). At the opposite end of the spectrum, bacterial orders with OTUs with low overall abundance included Burkholderiales (Betaproteobacteria), Oceanospirillales (Gammaproteobacteria) and Bifidobacteriales (Actinobacteria) (Table 2).

Bottom Line: The most abundant and prevalent (occurring in 92% of the samples) bacterial OTU, based on 16S rRNA sequences, closely matched that of the previously described Betaproteobacteria species Snodgrassella alvi.Bacteria that were first described in bee-related external environments dominated a number of gut bacterial communities, suggesting that they are not strictly dependent on the internal gut environment.Using light and fluorescent microscopy, we demonstrated that the gut bacteria form a biofilm on the internal epithelial surface of the ileum, corroborating results obtained from Apis mellifera.

View Article: PubMed Central - PubMed

Affiliation: Department of Entomology, University of Illinois, Urbana, IL, 61801, United States of America.

ABSTRACT
Gut bacterial communities of bumble bees are correlated with defense against pathogens. Further understanding this host-microbe association is vitally important as bumble bees are currently experiencing global population declines, potentially due in part to emergent diseases. In this study, we used pyrosequencing and community fingerprinting (ARISA) to characterize the gut microbial communities of nine bumble species from across the Bombus phylogeny. Overall, we delimited 74 bacterial taxa (operational taxonomic units or OTUs) belonging to Betaproteobacteria, Gammaproteobacteria, Bacilli, Actinobacteria, Flavobacteria and Alphaproteobacteria. Each bacterial community was taxonomically simple, containing an average of 1.9 common (relative abundance per sample > 5%) bacterial OTUs. The most abundant and prevalent (occurring in 92% of the samples) bacterial OTU, based on 16S rRNA sequences, closely matched that of the previously described Betaproteobacteria species Snodgrassella alvi. Bacteria that were first described in bee-related external environments dominated a number of gut bacterial communities, suggesting that they are not strictly dependent on the internal gut environment. The ARISA data showed a correlation between bacterial community structures and the geographic locations where the bees were sampled, suggesting that at least a subset of the bacterial species may be transmitted environmentally. Using light and fluorescent microscopy, we demonstrated that the gut bacteria form a biofilm on the internal epithelial surface of the ileum, corroborating results obtained from Apis mellifera.

Show MeSH
Related in: MedlinePlus