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The effect on the transcriptome of Anemone coronaria following infection with rust (Tranzschelia discolor).

Laura M, Borghi C, Bobbio V, Allavena A - PLoS ONE (2015)

Bottom Line: Unigene annotation showed that 50.5% of the predicted translation products shared significant homology with protein sequences in GenBank.Only one R gene (PGIP) was up-regulated.The DTA genes identified here should provide a basis for understanding the A. coronaria/T. discolor interaction and leads for biotechnology-based disease resistance breeding.

View Article: PubMed Central - PubMed

Affiliation: CRA-Unità di Ricerca per la Floricoltura e le Specie Ornamentali, Corso Inglesi 508, 18038 Sanremo (IM), Italy.

ABSTRACT
In order to understand plant/pathogen interaction, the transcriptome of uninfected (1S) and infected (2I) plant was sequenced at 3'end by the GS FLX 454 platform. De novo assembly of high-quality reads generated 27,231 contigs leaving 37,191 singletons in the 1S and 38,393 in the 2I libraries. ESTcalc tool suggested that 71% of the transcriptome had been captured, with 99% of the genes present being represented by at least one read. Unigene annotation showed that 50.5% of the predicted translation products shared significant homology with protein sequences in GenBank. In all 253 differential transcript abundance (DTAs) were in higher abundance and 52 in lower abundance in the 2I library. 128 higher abundance DTA genes were of fungal origin and 49 were clearly plant sequences. A tBLASTn-based search of the sequences using as query the full length predicted polypeptide product of 50 R genes identified 16 R gene products. Only one R gene (PGIP) was up-regulated. The response of the plant to fungal invasion included the up-regulation of several pathogenesis related protein (PR) genes involved in JA signaling and other genes associated with defense response and down regulation of cell wall associated genes, non-race-specific disease resistance1 (NDR1) and other genes like myb, presqualene diphosphate phosphatase (PSDPase), a UDP-glycosyltransferase 74E2-like (UGT). The DTA genes identified here should provide a basis for understanding the A. coronaria/T. discolor interaction and leads for biotechnology-based disease resistance breeding.

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Aligment of NDR1 proteins.Anemone coronaria contigs 6057 and 9288 are aligned with selected member of NDR1 proteins. Motif 1, motif 2 and 3 of NDR1/HIN-like (NHL) protein superfamily are highlighted in the boxes.
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pone.0118565.g007: Aligment of NDR1 proteins.Anemone coronaria contigs 6057 and 9288 are aligned with selected member of NDR1 proteins. Motif 1, motif 2 and 3 of NDR1/HIN-like (NHL) protein superfamily are highlighted in the boxes.

Mentions: Two genes encoding a product with significant homology to NON-RACE-SPECIFIC DISEASE RESISTANCE1 (NDR1), a plasma membrane-localized protein were down regulated. NDR1 is involved in the maintenance of the integrity of the cell wall/plasma membrane connection and represents a key signaling component during pathogen infection [117]. In Arabidopsis a member of the CC-NBS-LRR R protein require the signaling gene NDR1 for full activity [118]. Alignment of A. coronaria NDR1 deduced protein with the NCBI nr database proteins identifies motif 2 and 3 of the three NDR1/ HIN1-like (NHL) protein superfamily [119,120]. Motif 1 was not covered by the A. coronaria sequence (Fig. 7). Phylogenetic analysis together with down regulation during T. discolor infection provide evidence that A. coronaria NDR1 genes are credible candidate of the fungus to establish biotrophic relationship.


The effect on the transcriptome of Anemone coronaria following infection with rust (Tranzschelia discolor).

Laura M, Borghi C, Bobbio V, Allavena A - PLoS ONE (2015)

Aligment of NDR1 proteins.Anemone coronaria contigs 6057 and 9288 are aligned with selected member of NDR1 proteins. Motif 1, motif 2 and 3 of NDR1/HIN-like (NHL) protein superfamily are highlighted in the boxes.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4359109&req=5

pone.0118565.g007: Aligment of NDR1 proteins.Anemone coronaria contigs 6057 and 9288 are aligned with selected member of NDR1 proteins. Motif 1, motif 2 and 3 of NDR1/HIN-like (NHL) protein superfamily are highlighted in the boxes.
Mentions: Two genes encoding a product with significant homology to NON-RACE-SPECIFIC DISEASE RESISTANCE1 (NDR1), a plasma membrane-localized protein were down regulated. NDR1 is involved in the maintenance of the integrity of the cell wall/plasma membrane connection and represents a key signaling component during pathogen infection [117]. In Arabidopsis a member of the CC-NBS-LRR R protein require the signaling gene NDR1 for full activity [118]. Alignment of A. coronaria NDR1 deduced protein with the NCBI nr database proteins identifies motif 2 and 3 of the three NDR1/ HIN1-like (NHL) protein superfamily [119,120]. Motif 1 was not covered by the A. coronaria sequence (Fig. 7). Phylogenetic analysis together with down regulation during T. discolor infection provide evidence that A. coronaria NDR1 genes are credible candidate of the fungus to establish biotrophic relationship.

Bottom Line: Unigene annotation showed that 50.5% of the predicted translation products shared significant homology with protein sequences in GenBank.Only one R gene (PGIP) was up-regulated.The DTA genes identified here should provide a basis for understanding the A. coronaria/T. discolor interaction and leads for biotechnology-based disease resistance breeding.

View Article: PubMed Central - PubMed

Affiliation: CRA-Unità di Ricerca per la Floricoltura e le Specie Ornamentali, Corso Inglesi 508, 18038 Sanremo (IM), Italy.

ABSTRACT
In order to understand plant/pathogen interaction, the transcriptome of uninfected (1S) and infected (2I) plant was sequenced at 3'end by the GS FLX 454 platform. De novo assembly of high-quality reads generated 27,231 contigs leaving 37,191 singletons in the 1S and 38,393 in the 2I libraries. ESTcalc tool suggested that 71% of the transcriptome had been captured, with 99% of the genes present being represented by at least one read. Unigene annotation showed that 50.5% of the predicted translation products shared significant homology with protein sequences in GenBank. In all 253 differential transcript abundance (DTAs) were in higher abundance and 52 in lower abundance in the 2I library. 128 higher abundance DTA genes were of fungal origin and 49 were clearly plant sequences. A tBLASTn-based search of the sequences using as query the full length predicted polypeptide product of 50 R genes identified 16 R gene products. Only one R gene (PGIP) was up-regulated. The response of the plant to fungal invasion included the up-regulation of several pathogenesis related protein (PR) genes involved in JA signaling and other genes associated with defense response and down regulation of cell wall associated genes, non-race-specific disease resistance1 (NDR1) and other genes like myb, presqualene diphosphate phosphatase (PSDPase), a UDP-glycosyltransferase 74E2-like (UGT). The DTA genes identified here should provide a basis for understanding the A. coronaria/T. discolor interaction and leads for biotechnology-based disease resistance breeding.

Show MeSH
Related in: MedlinePlus