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The effect on the transcriptome of Anemone coronaria following infection with rust (Tranzschelia discolor).

Laura M, Borghi C, Bobbio V, Allavena A - PLoS ONE (2015)

Bottom Line: Unigene annotation showed that 50.5% of the predicted translation products shared significant homology with protein sequences in GenBank.Only one R gene (PGIP) was up-regulated.The DTA genes identified here should provide a basis for understanding the A. coronaria/T. discolor interaction and leads for biotechnology-based disease resistance breeding.

View Article: PubMed Central - PubMed

Affiliation: CRA-Unità di Ricerca per la Floricoltura e le Specie Ornamentali, Corso Inglesi 508, 18038 Sanremo (IM), Italy.

ABSTRACT
In order to understand plant/pathogen interaction, the transcriptome of uninfected (1S) and infected (2I) plant was sequenced at 3'end by the GS FLX 454 platform. De novo assembly of high-quality reads generated 27,231 contigs leaving 37,191 singletons in the 1S and 38,393 in the 2I libraries. ESTcalc tool suggested that 71% of the transcriptome had been captured, with 99% of the genes present being represented by at least one read. Unigene annotation showed that 50.5% of the predicted translation products shared significant homology with protein sequences in GenBank. In all 253 differential transcript abundance (DTAs) were in higher abundance and 52 in lower abundance in the 2I library. 128 higher abundance DTA genes were of fungal origin and 49 were clearly plant sequences. A tBLASTn-based search of the sequences using as query the full length predicted polypeptide product of 50 R genes identified 16 R gene products. Only one R gene (PGIP) was up-regulated. The response of the plant to fungal invasion included the up-regulation of several pathogenesis related protein (PR) genes involved in JA signaling and other genes associated with defense response and down regulation of cell wall associated genes, non-race-specific disease resistance1 (NDR1) and other genes like myb, presqualene diphosphate phosphatase (PSDPase), a UDP-glycosyltransferase 74E2-like (UGT). The DTA genes identified here should provide a basis for understanding the A. coronaria/T. discolor interaction and leads for biotechnology-based disease resistance breeding.

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KEGG pathway of α-linoleic acid metabolism.Anemone coronaria transcrips involved in jasmonic acid metablolic pathway are highlighted by grey tone. 12-oxophytodienoate reductase 2 (EC: 5.3.99.6) and acyl-CoA oxidase (ACX) are upregulated during Tranzschelia discolor infection.
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pone.0118565.g004: KEGG pathway of α-linoleic acid metabolism.Anemone coronaria transcrips involved in jasmonic acid metablolic pathway are highlighted by grey tone. 12-oxophytodienoate reductase 2 (EC: 5.3.99.6) and acyl-CoA oxidase (ACX) are upregulated during Tranzschelia discolor infection.

Mentions: Genes involved jasmonate (JA) signaling. Among the genes up-regulated in the infected plants were six which encode various proteins involved in JA signaling; these included those encoding 12-oxophytodienoate reductase 2 [63] and acyl-CoA oxidase [64], which are both components of JA synthesis, two JA-induced proteins, one JA signaling repressor (TIFY 3B, also known as JAZ12) and strictosidine synthase 1 [65]. JAZ proteins were degraded on perception of jasmonyl-isoleucine (JA-Ile, active form of JA) allowing the JA-Ile dependent gene expression [66,67]. Strictosidine synthase 1, a key enzyme in alkaloid biosynthesis, was induced by plant defence signalling compounds, such as salicylic acid (SA), ethylene and methyl jasmonate [65]. The Kegg analysis of the jasmonate biosynthetic pathway proves that six genes, in addition to the two up regulated, were identified by the trascriptome sequencing (Fig. 4).


The effect on the transcriptome of Anemone coronaria following infection with rust (Tranzschelia discolor).

Laura M, Borghi C, Bobbio V, Allavena A - PLoS ONE (2015)

KEGG pathway of α-linoleic acid metabolism.Anemone coronaria transcrips involved in jasmonic acid metablolic pathway are highlighted by grey tone. 12-oxophytodienoate reductase 2 (EC: 5.3.99.6) and acyl-CoA oxidase (ACX) are upregulated during Tranzschelia discolor infection.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4359109&req=5

pone.0118565.g004: KEGG pathway of α-linoleic acid metabolism.Anemone coronaria transcrips involved in jasmonic acid metablolic pathway are highlighted by grey tone. 12-oxophytodienoate reductase 2 (EC: 5.3.99.6) and acyl-CoA oxidase (ACX) are upregulated during Tranzschelia discolor infection.
Mentions: Genes involved jasmonate (JA) signaling. Among the genes up-regulated in the infected plants were six which encode various proteins involved in JA signaling; these included those encoding 12-oxophytodienoate reductase 2 [63] and acyl-CoA oxidase [64], which are both components of JA synthesis, two JA-induced proteins, one JA signaling repressor (TIFY 3B, also known as JAZ12) and strictosidine synthase 1 [65]. JAZ proteins were degraded on perception of jasmonyl-isoleucine (JA-Ile, active form of JA) allowing the JA-Ile dependent gene expression [66,67]. Strictosidine synthase 1, a key enzyme in alkaloid biosynthesis, was induced by plant defence signalling compounds, such as salicylic acid (SA), ethylene and methyl jasmonate [65]. The Kegg analysis of the jasmonate biosynthetic pathway proves that six genes, in addition to the two up regulated, were identified by the trascriptome sequencing (Fig. 4).

Bottom Line: Unigene annotation showed that 50.5% of the predicted translation products shared significant homology with protein sequences in GenBank.Only one R gene (PGIP) was up-regulated.The DTA genes identified here should provide a basis for understanding the A. coronaria/T. discolor interaction and leads for biotechnology-based disease resistance breeding.

View Article: PubMed Central - PubMed

Affiliation: CRA-Unità di Ricerca per la Floricoltura e le Specie Ornamentali, Corso Inglesi 508, 18038 Sanremo (IM), Italy.

ABSTRACT
In order to understand plant/pathogen interaction, the transcriptome of uninfected (1S) and infected (2I) plant was sequenced at 3'end by the GS FLX 454 platform. De novo assembly of high-quality reads generated 27,231 contigs leaving 37,191 singletons in the 1S and 38,393 in the 2I libraries. ESTcalc tool suggested that 71% of the transcriptome had been captured, with 99% of the genes present being represented by at least one read. Unigene annotation showed that 50.5% of the predicted translation products shared significant homology with protein sequences in GenBank. In all 253 differential transcript abundance (DTAs) were in higher abundance and 52 in lower abundance in the 2I library. 128 higher abundance DTA genes were of fungal origin and 49 were clearly plant sequences. A tBLASTn-based search of the sequences using as query the full length predicted polypeptide product of 50 R genes identified 16 R gene products. Only one R gene (PGIP) was up-regulated. The response of the plant to fungal invasion included the up-regulation of several pathogenesis related protein (PR) genes involved in JA signaling and other genes associated with defense response and down regulation of cell wall associated genes, non-race-specific disease resistance1 (NDR1) and other genes like myb, presqualene diphosphate phosphatase (PSDPase), a UDP-glycosyltransferase 74E2-like (UGT). The DTA genes identified here should provide a basis for understanding the A. coronaria/T. discolor interaction and leads for biotechnology-based disease resistance breeding.

Show MeSH
Related in: MedlinePlus