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The effect on the transcriptome of Anemone coronaria following infection with rust (Tranzschelia discolor).

Laura M, Borghi C, Bobbio V, Allavena A - PLoS ONE (2015)

Bottom Line: Unigene annotation showed that 50.5% of the predicted translation products shared significant homology with protein sequences in GenBank.Only one R gene (PGIP) was up-regulated.The DTA genes identified here should provide a basis for understanding the A. coronaria/T. discolor interaction and leads for biotechnology-based disease resistance breeding.

View Article: PubMed Central - PubMed

Affiliation: CRA-Unità di Ricerca per la Floricoltura e le Specie Ornamentali, Corso Inglesi 508, 18038 Sanremo (IM), Italy.

ABSTRACT
In order to understand plant/pathogen interaction, the transcriptome of uninfected (1S) and infected (2I) plant was sequenced at 3'end by the GS FLX 454 platform. De novo assembly of high-quality reads generated 27,231 contigs leaving 37,191 singletons in the 1S and 38,393 in the 2I libraries. ESTcalc tool suggested that 71% of the transcriptome had been captured, with 99% of the genes present being represented by at least one read. Unigene annotation showed that 50.5% of the predicted translation products shared significant homology with protein sequences in GenBank. In all 253 differential transcript abundance (DTAs) were in higher abundance and 52 in lower abundance in the 2I library. 128 higher abundance DTA genes were of fungal origin and 49 were clearly plant sequences. A tBLASTn-based search of the sequences using as query the full length predicted polypeptide product of 50 R genes identified 16 R gene products. Only one R gene (PGIP) was up-regulated. The response of the plant to fungal invasion included the up-regulation of several pathogenesis related protein (PR) genes involved in JA signaling and other genes associated with defense response and down regulation of cell wall associated genes, non-race-specific disease resistance1 (NDR1) and other genes like myb, presqualene diphosphate phosphatase (PSDPase), a UDP-glycosyltransferase 74E2-like (UGT). The DTA genes identified here should provide a basis for understanding the A. coronaria/T. discolor interaction and leads for biotechnology-based disease resistance breeding.

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Healthy and Tranzschelia discolor infected plant.In comparison to healthy plant (A), during biotropich relationship, infected plant displays plethoric vegetation with robust leaf stems and curly leaf lamina. Flowering is strongly repressed.
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pone.0118565.g001: Healthy and Tranzschelia discolor infected plant.In comparison to healthy plant (A), during biotropich relationship, infected plant displays plethoric vegetation with robust leaf stems and curly leaf lamina. Flowering is strongly repressed.

Mentions: A. coronaria plants (cv ‘Tetraelite’ blue) were grown under shade netting. Thirty healthy and thirty T. discolor infected plants were monitored throughout their life cycle for disease symptoms. Early infected plants were easily identified by plethoric vegetation, robust, erect leaf stems and thick, slightly curled leaf lamina. Leaves of infected plants were harvested as soon as plant showed disease symptoms. This time point covers leaf invasion by hyphae from the plant rhizomes under real field condition. Healthy leaves of the same age were harvested from uninfected plants (Fig. 1). Leaf tissues was snap-frozen in liquid nitrogen and stored at -80°C until required. Total RNA was isolated from 100 mg of frozen leaf using an RNeasy Plant Mini kit (QIAGEN GmbH, Hilden, Germany) and treated with recombinant DNase I (QIAGEN) within the column, following the manufacturer’s protocol. The concentration of recovered RNA was estimated using a Nanodrop 2000 device (Thermo Fisher Scientific Inc., Wilmington, DE, U.S.A.) and its integrity assessed using a Total RNA Stdsens chip (Experion system, Biorad, Hercules, CA, USA). High quality RNAs from five uninfected plants were combined to form the “1S” pool and similarly from five infected ones to form the “2I” pool.


The effect on the transcriptome of Anemone coronaria following infection with rust (Tranzschelia discolor).

Laura M, Borghi C, Bobbio V, Allavena A - PLoS ONE (2015)

Healthy and Tranzschelia discolor infected plant.In comparison to healthy plant (A), during biotropich relationship, infected plant displays plethoric vegetation with robust leaf stems and curly leaf lamina. Flowering is strongly repressed.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4359109&req=5

pone.0118565.g001: Healthy and Tranzschelia discolor infected plant.In comparison to healthy plant (A), during biotropich relationship, infected plant displays plethoric vegetation with robust leaf stems and curly leaf lamina. Flowering is strongly repressed.
Mentions: A. coronaria plants (cv ‘Tetraelite’ blue) were grown under shade netting. Thirty healthy and thirty T. discolor infected plants were monitored throughout their life cycle for disease symptoms. Early infected plants were easily identified by plethoric vegetation, robust, erect leaf stems and thick, slightly curled leaf lamina. Leaves of infected plants were harvested as soon as plant showed disease symptoms. This time point covers leaf invasion by hyphae from the plant rhizomes under real field condition. Healthy leaves of the same age were harvested from uninfected plants (Fig. 1). Leaf tissues was snap-frozen in liquid nitrogen and stored at -80°C until required. Total RNA was isolated from 100 mg of frozen leaf using an RNeasy Plant Mini kit (QIAGEN GmbH, Hilden, Germany) and treated with recombinant DNase I (QIAGEN) within the column, following the manufacturer’s protocol. The concentration of recovered RNA was estimated using a Nanodrop 2000 device (Thermo Fisher Scientific Inc., Wilmington, DE, U.S.A.) and its integrity assessed using a Total RNA Stdsens chip (Experion system, Biorad, Hercules, CA, USA). High quality RNAs from five uninfected plants were combined to form the “1S” pool and similarly from five infected ones to form the “2I” pool.

Bottom Line: Unigene annotation showed that 50.5% of the predicted translation products shared significant homology with protein sequences in GenBank.Only one R gene (PGIP) was up-regulated.The DTA genes identified here should provide a basis for understanding the A. coronaria/T. discolor interaction and leads for biotechnology-based disease resistance breeding.

View Article: PubMed Central - PubMed

Affiliation: CRA-Unità di Ricerca per la Floricoltura e le Specie Ornamentali, Corso Inglesi 508, 18038 Sanremo (IM), Italy.

ABSTRACT
In order to understand plant/pathogen interaction, the transcriptome of uninfected (1S) and infected (2I) plant was sequenced at 3'end by the GS FLX 454 platform. De novo assembly of high-quality reads generated 27,231 contigs leaving 37,191 singletons in the 1S and 38,393 in the 2I libraries. ESTcalc tool suggested that 71% of the transcriptome had been captured, with 99% of the genes present being represented by at least one read. Unigene annotation showed that 50.5% of the predicted translation products shared significant homology with protein sequences in GenBank. In all 253 differential transcript abundance (DTAs) were in higher abundance and 52 in lower abundance in the 2I library. 128 higher abundance DTA genes were of fungal origin and 49 were clearly plant sequences. A tBLASTn-based search of the sequences using as query the full length predicted polypeptide product of 50 R genes identified 16 R gene products. Only one R gene (PGIP) was up-regulated. The response of the plant to fungal invasion included the up-regulation of several pathogenesis related protein (PR) genes involved in JA signaling and other genes associated with defense response and down regulation of cell wall associated genes, non-race-specific disease resistance1 (NDR1) and other genes like myb, presqualene diphosphate phosphatase (PSDPase), a UDP-glycosyltransferase 74E2-like (UGT). The DTA genes identified here should provide a basis for understanding the A. coronaria/T. discolor interaction and leads for biotechnology-based disease resistance breeding.

Show MeSH
Related in: MedlinePlus