Limits...
Protein arginine methyltransferase 5 is a key regulator of the MYCN oncoprotein in neuroblastoma cells.

Park JH, Szemes M, Vieira GC, Melegh Z, Malik S, Heesom KJ, Von Wallwitz-Freitas L, Greenhough A, Brown KW, Zheng YG, Catchpoole D, Deery MJ, Malik K - Mol Oncol (2014)

Bottom Line: PRMT5 knockdown in MYCN-overexpressing cells, including the SHEP-21N cell-line with inducible MYCN expression leads to a dramatic decrease in MYCN protein and MYCN-associated cell-death in SHEP-21N cells.By using liquid chromatography - tandem mass spectrometry (LC-MS/MS) analysis of immunoprecipitated MYCN protein, we identified several potential sites of arginine dimethylation on the MYCN protein.Together our studies implicate PRMT5 in a novel mode of MYCN post-translational regulation and suggest PRMT5 plays a major role in NB tumorigenesis.

View Article: PubMed Central - PubMed

Affiliation: Cancer Epigenetics Laboratory University of Bristol, Bristol BS8 1TD, UK.

Show MeSH

Related in: MedlinePlus

MYCN protein sequence and peptide coverage by LC-MS/MS is shown by shading and the number of peptides detected. Arginines predicted to be mono- and/or dimethylated are marked by red boxes, whereas the arginine predicted to be monomethylated is indicated by a stippled red box.
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4359099&req=5

undfig6: MYCN protein sequence and peptide coverage by LC-MS/MS is shown by shading and the number of peptides detected. Arginines predicted to be mono- and/or dimethylated are marked by red boxes, whereas the arginine predicted to be monomethylated is indicated by a stippled red box.


Protein arginine methyltransferase 5 is a key regulator of the MYCN oncoprotein in neuroblastoma cells.

Park JH, Szemes M, Vieira GC, Melegh Z, Malik S, Heesom KJ, Von Wallwitz-Freitas L, Greenhough A, Brown KW, Zheng YG, Catchpoole D, Deery MJ, Malik K - Mol Oncol (2014)

MYCN protein sequence and peptide coverage by LC-MS/MS is shown by shading and the number of peptides detected. Arginines predicted to be mono- and/or dimethylated are marked by red boxes, whereas the arginine predicted to be monomethylated is indicated by a stippled red box.
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4359099&req=5

undfig6: MYCN protein sequence and peptide coverage by LC-MS/MS is shown by shading and the number of peptides detected. Arginines predicted to be mono- and/or dimethylated are marked by red boxes, whereas the arginine predicted to be monomethylated is indicated by a stippled red box.
Bottom Line: PRMT5 knockdown in MYCN-overexpressing cells, including the SHEP-21N cell-line with inducible MYCN expression leads to a dramatic decrease in MYCN protein and MYCN-associated cell-death in SHEP-21N cells.By using liquid chromatography - tandem mass spectrometry (LC-MS/MS) analysis of immunoprecipitated MYCN protein, we identified several potential sites of arginine dimethylation on the MYCN protein.Together our studies implicate PRMT5 in a novel mode of MYCN post-translational regulation and suggest PRMT5 plays a major role in NB tumorigenesis.

View Article: PubMed Central - PubMed

Affiliation: Cancer Epigenetics Laboratory University of Bristol, Bristol BS8 1TD, UK.

Show MeSH
Related in: MedlinePlus