Limits...
The NDST gene family in zebrafish: role of NDST1B in pharyngeal arch formation.

Filipek-Górniok B, Carlsson P, Haitina T, Habicher J, Ledin J, Kjellén L - PLoS ONE (2015)

Bottom Line: Interestingly, the single zebrafish orthologue ndst3, is equally similar to tetrapod Ndst3 and Ndst4.It is likely that a local duplication in the common ancestor of lobe-finned fish and tetrapods gave rise to these two genes.All zebrafish Ndst genes showed distinct but partially overlapping expression patterns during embryonic development.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Medical Biochemistry and Microbiology, Science for Life Laboratory, Uppsala University, Husargatan 3, PO Box 582, SE-751 23, Uppsala, Sweden.

ABSTRACT
Heparan sulfate (HS) proteoglycans are ubiquitous components of the extracellular matrix and plasma membrane of metazoans. The sulfation pattern of the HS glycosaminoglycan chain is characteristic for each tissue and changes during development. The glucosaminyl N-deacetylase/N-sulfotransferase (NDST) enzymes catalyze N-deacetylation and N-sulfation during HS biosynthesis and have a key role in designing the sulfation pattern. We here report on the presence of five NDST genes in zebrafish. Zebrafish ndst1a, ndst1b, ndst2a and ndst2b represent duplicated mammalian orthologues of NDST1 and NDST2 that arose through teleost specific genome duplication. Interestingly, the single zebrafish orthologue ndst3, is equally similar to tetrapod Ndst3 and Ndst4. It is likely that a local duplication in the common ancestor of lobe-finned fish and tetrapods gave rise to these two genes. All zebrafish Ndst genes showed distinct but partially overlapping expression patterns during embryonic development. Morpholino knockdown of ndst1b resulted in delayed development, craniofacial cartilage abnormalities, shortened body and pectoral fin length, resembling some of the features of the Ndst1 mouse knockout.

Show MeSH

Related in: MedlinePlus

Expression of zebrafish ndst genes at 72 hpf.Lateral views of larvae (first column to the left), dorsal views of larval heads (second column to the left) and transversal sections showing the expression patterns of ndst1a: A-D; ndst1b: E-H; ndst2a: I-L; ndst2b: N-Q; and ndst3: R-U. Positions of the sections are indicated with arrow heads. Abbreviations used: h, hindbrain; pf, pectoral fins.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4359090&req=5

pone.0119040.g005: Expression of zebrafish ndst genes at 72 hpf.Lateral views of larvae (first column to the left), dorsal views of larval heads (second column to the left) and transversal sections showing the expression patterns of ndst1a: A-D; ndst1b: E-H; ndst2a: I-L; ndst2b: N-Q; and ndst3: R-U. Positions of the sections are indicated with arrow heads. Abbreviations used: h, hindbrain; pf, pectoral fins.

Mentions: ndst1a showed an ubiquitous expression pattern at early stages (Fig. 3A-D), whereas at 24 hpf expression was restricted mostly to the posterior part of optic tectum (Fig. 4A-B), persistent also at 38 and 50 hpf (Fig. 4C-E). At 72 hpf weak staining was observed in the head region as well as in the developing pectoral fins (Fig. 5A-D) ndst1b was the main isoform expressed in the somites already at the 5 somite stage (Fig. 3F) and was later peaking in the myotome at 24 hpf (Fig. 4F). At 24 hpf, ndst1b expression was observed in rhombomeres and weaker staining was seen in the region of the migratory neural crest (Fig. 4F-G). At 50 hpf expression was observed in brain tissues, showing specific staining in hindbrain and along the borders of the optic tectum (Fig. 4I-J). The same trend was observed at 72 hpf, with ndst1b dominant expression in the rhombomeres and weak expression in the pectoral fins (Fig. 5E-H).


The NDST gene family in zebrafish: role of NDST1B in pharyngeal arch formation.

Filipek-Górniok B, Carlsson P, Haitina T, Habicher J, Ledin J, Kjellén L - PLoS ONE (2015)

Expression of zebrafish ndst genes at 72 hpf.Lateral views of larvae (first column to the left), dorsal views of larval heads (second column to the left) and transversal sections showing the expression patterns of ndst1a: A-D; ndst1b: E-H; ndst2a: I-L; ndst2b: N-Q; and ndst3: R-U. Positions of the sections are indicated with arrow heads. Abbreviations used: h, hindbrain; pf, pectoral fins.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4359090&req=5

pone.0119040.g005: Expression of zebrafish ndst genes at 72 hpf.Lateral views of larvae (first column to the left), dorsal views of larval heads (second column to the left) and transversal sections showing the expression patterns of ndst1a: A-D; ndst1b: E-H; ndst2a: I-L; ndst2b: N-Q; and ndst3: R-U. Positions of the sections are indicated with arrow heads. Abbreviations used: h, hindbrain; pf, pectoral fins.
Mentions: ndst1a showed an ubiquitous expression pattern at early stages (Fig. 3A-D), whereas at 24 hpf expression was restricted mostly to the posterior part of optic tectum (Fig. 4A-B), persistent also at 38 and 50 hpf (Fig. 4C-E). At 72 hpf weak staining was observed in the head region as well as in the developing pectoral fins (Fig. 5A-D) ndst1b was the main isoform expressed in the somites already at the 5 somite stage (Fig. 3F) and was later peaking in the myotome at 24 hpf (Fig. 4F). At 24 hpf, ndst1b expression was observed in rhombomeres and weaker staining was seen in the region of the migratory neural crest (Fig. 4F-G). At 50 hpf expression was observed in brain tissues, showing specific staining in hindbrain and along the borders of the optic tectum (Fig. 4I-J). The same trend was observed at 72 hpf, with ndst1b dominant expression in the rhombomeres and weak expression in the pectoral fins (Fig. 5E-H).

Bottom Line: Interestingly, the single zebrafish orthologue ndst3, is equally similar to tetrapod Ndst3 and Ndst4.It is likely that a local duplication in the common ancestor of lobe-finned fish and tetrapods gave rise to these two genes.All zebrafish Ndst genes showed distinct but partially overlapping expression patterns during embryonic development.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Medical Biochemistry and Microbiology, Science for Life Laboratory, Uppsala University, Husargatan 3, PO Box 582, SE-751 23, Uppsala, Sweden.

ABSTRACT
Heparan sulfate (HS) proteoglycans are ubiquitous components of the extracellular matrix and plasma membrane of metazoans. The sulfation pattern of the HS glycosaminoglycan chain is characteristic for each tissue and changes during development. The glucosaminyl N-deacetylase/N-sulfotransferase (NDST) enzymes catalyze N-deacetylation and N-sulfation during HS biosynthesis and have a key role in designing the sulfation pattern. We here report on the presence of five NDST genes in zebrafish. Zebrafish ndst1a, ndst1b, ndst2a and ndst2b represent duplicated mammalian orthologues of NDST1 and NDST2 that arose through teleost specific genome duplication. Interestingly, the single zebrafish orthologue ndst3, is equally similar to tetrapod Ndst3 and Ndst4. It is likely that a local duplication in the common ancestor of lobe-finned fish and tetrapods gave rise to these two genes. All zebrafish Ndst genes showed distinct but partially overlapping expression patterns during embryonic development. Morpholino knockdown of ndst1b resulted in delayed development, craniofacial cartilage abnormalities, shortened body and pectoral fin length, resembling some of the features of the Ndst1 mouse knockout.

Show MeSH
Related in: MedlinePlus