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The NDST gene family in zebrafish: role of NDST1B in pharyngeal arch formation.

Filipek-Górniok B, Carlsson P, Haitina T, Habicher J, Ledin J, Kjellén L - PLoS ONE (2015)

Bottom Line: Interestingly, the single zebrafish orthologue ndst3, is equally similar to tetrapod Ndst3 and Ndst4.It is likely that a local duplication in the common ancestor of lobe-finned fish and tetrapods gave rise to these two genes.All zebrafish Ndst genes showed distinct but partially overlapping expression patterns during embryonic development.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Medical Biochemistry and Microbiology, Science for Life Laboratory, Uppsala University, Husargatan 3, PO Box 582, SE-751 23, Uppsala, Sweden.

ABSTRACT
Heparan sulfate (HS) proteoglycans are ubiquitous components of the extracellular matrix and plasma membrane of metazoans. The sulfation pattern of the HS glycosaminoglycan chain is characteristic for each tissue and changes during development. The glucosaminyl N-deacetylase/N-sulfotransferase (NDST) enzymes catalyze N-deacetylation and N-sulfation during HS biosynthesis and have a key role in designing the sulfation pattern. We here report on the presence of five NDST genes in zebrafish. Zebrafish ndst1a, ndst1b, ndst2a and ndst2b represent duplicated mammalian orthologues of NDST1 and NDST2 that arose through teleost specific genome duplication. Interestingly, the single zebrafish orthologue ndst3, is equally similar to tetrapod Ndst3 and Ndst4. It is likely that a local duplication in the common ancestor of lobe-finned fish and tetrapods gave rise to these two genes. All zebrafish Ndst genes showed distinct but partially overlapping expression patterns during embryonic development. Morpholino knockdown of ndst1b resulted in delayed development, craniofacial cartilage abnormalities, shortened body and pectoral fin length, resembling some of the features of the Ndst1 mouse knockout.

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Expression of zebrafish ndst genes at 24, 38 and 50 hpf.Lateral and dorsal view of the embryos, or head region of the embryo. Embryonic expression patterns are shown for ndst1a: A-E; ndst1b: F-J; ndst2a: K-O; ndst2b: P-T; ndst3: U-Y. Abbreviations used: h, hindbrain; icm, intermediate cell mass (blood islands); op, olfactory placode and olfactory bulb; r, rhombomeres; s:, somites; sc, spinal cord; t, tectum; te, telencephalon.
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pone.0119040.g004: Expression of zebrafish ndst genes at 24, 38 and 50 hpf.Lateral and dorsal view of the embryos, or head region of the embryo. Embryonic expression patterns are shown for ndst1a: A-E; ndst1b: F-J; ndst2a: K-O; ndst2b: P-T; ndst3: U-Y. Abbreviations used: h, hindbrain; icm, intermediate cell mass (blood islands); op, olfactory placode and olfactory bulb; r, rhombomeres; s:, somites; sc, spinal cord; t, tectum; te, telencephalon.

Mentions: Each of the probes showed unique staining in the head region throughout investigated developmental stages. However, only three of the genes were transcribed in the trunk at 24 hours post fertilization (hpf), more specifically in the spinal cord (ndst3) and in the somites (ndst1a and ndst1b) (Fig. 4). ndst3 expression in the spinal cord was persisting also at 50 hpf whereas myotomal staining of ndst1b was very weak at this time point (Fig. 4).


The NDST gene family in zebrafish: role of NDST1B in pharyngeal arch formation.

Filipek-Górniok B, Carlsson P, Haitina T, Habicher J, Ledin J, Kjellén L - PLoS ONE (2015)

Expression of zebrafish ndst genes at 24, 38 and 50 hpf.Lateral and dorsal view of the embryos, or head region of the embryo. Embryonic expression patterns are shown for ndst1a: A-E; ndst1b: F-J; ndst2a: K-O; ndst2b: P-T; ndst3: U-Y. Abbreviations used: h, hindbrain; icm, intermediate cell mass (blood islands); op, olfactory placode and olfactory bulb; r, rhombomeres; s:, somites; sc, spinal cord; t, tectum; te, telencephalon.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4359090&req=5

pone.0119040.g004: Expression of zebrafish ndst genes at 24, 38 and 50 hpf.Lateral and dorsal view of the embryos, or head region of the embryo. Embryonic expression patterns are shown for ndst1a: A-E; ndst1b: F-J; ndst2a: K-O; ndst2b: P-T; ndst3: U-Y. Abbreviations used: h, hindbrain; icm, intermediate cell mass (blood islands); op, olfactory placode and olfactory bulb; r, rhombomeres; s:, somites; sc, spinal cord; t, tectum; te, telencephalon.
Mentions: Each of the probes showed unique staining in the head region throughout investigated developmental stages. However, only three of the genes were transcribed in the trunk at 24 hours post fertilization (hpf), more specifically in the spinal cord (ndst3) and in the somites (ndst1a and ndst1b) (Fig. 4). ndst3 expression in the spinal cord was persisting also at 50 hpf whereas myotomal staining of ndst1b was very weak at this time point (Fig. 4).

Bottom Line: Interestingly, the single zebrafish orthologue ndst3, is equally similar to tetrapod Ndst3 and Ndst4.It is likely that a local duplication in the common ancestor of lobe-finned fish and tetrapods gave rise to these two genes.All zebrafish Ndst genes showed distinct but partially overlapping expression patterns during embryonic development.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Medical Biochemistry and Microbiology, Science for Life Laboratory, Uppsala University, Husargatan 3, PO Box 582, SE-751 23, Uppsala, Sweden.

ABSTRACT
Heparan sulfate (HS) proteoglycans are ubiquitous components of the extracellular matrix and plasma membrane of metazoans. The sulfation pattern of the HS glycosaminoglycan chain is characteristic for each tissue and changes during development. The glucosaminyl N-deacetylase/N-sulfotransferase (NDST) enzymes catalyze N-deacetylation and N-sulfation during HS biosynthesis and have a key role in designing the sulfation pattern. We here report on the presence of five NDST genes in zebrafish. Zebrafish ndst1a, ndst1b, ndst2a and ndst2b represent duplicated mammalian orthologues of NDST1 and NDST2 that arose through teleost specific genome duplication. Interestingly, the single zebrafish orthologue ndst3, is equally similar to tetrapod Ndst3 and Ndst4. It is likely that a local duplication in the common ancestor of lobe-finned fish and tetrapods gave rise to these two genes. All zebrafish Ndst genes showed distinct but partially overlapping expression patterns during embryonic development. Morpholino knockdown of ndst1b resulted in delayed development, craniofacial cartilage abnormalities, shortened body and pectoral fin length, resembling some of the features of the Ndst1 mouse knockout.

Show MeSH
Related in: MedlinePlus