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The NDST gene family in zebrafish: role of NDST1B in pharyngeal arch formation.

Filipek-Górniok B, Carlsson P, Haitina T, Habicher J, Ledin J, Kjellén L - PLoS ONE (2015)

Bottom Line: Interestingly, the single zebrafish orthologue ndst3, is equally similar to tetrapod Ndst3 and Ndst4.It is likely that a local duplication in the common ancestor of lobe-finned fish and tetrapods gave rise to these two genes.All zebrafish Ndst genes showed distinct but partially overlapping expression patterns during embryonic development.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Medical Biochemistry and Microbiology, Science for Life Laboratory, Uppsala University, Husargatan 3, PO Box 582, SE-751 23, Uppsala, Sweden.

ABSTRACT
Heparan sulfate (HS) proteoglycans are ubiquitous components of the extracellular matrix and plasma membrane of metazoans. The sulfation pattern of the HS glycosaminoglycan chain is characteristic for each tissue and changes during development. The glucosaminyl N-deacetylase/N-sulfotransferase (NDST) enzymes catalyze N-deacetylation and N-sulfation during HS biosynthesis and have a key role in designing the sulfation pattern. We here report on the presence of five NDST genes in zebrafish. Zebrafish ndst1a, ndst1b, ndst2a and ndst2b represent duplicated mammalian orthologues of NDST1 and NDST2 that arose through teleost specific genome duplication. Interestingly, the single zebrafish orthologue ndst3, is equally similar to tetrapod Ndst3 and Ndst4. It is likely that a local duplication in the common ancestor of lobe-finned fish and tetrapods gave rise to these two genes. All zebrafish Ndst genes showed distinct but partially overlapping expression patterns during embryonic development. Morpholino knockdown of ndst1b resulted in delayed development, craniofacial cartilage abnormalities, shortened body and pectoral fin length, resembling some of the features of the Ndst1 mouse knockout.

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Expression of zebrafish ndst genes at early developmental stages.Lateral view of embryos at 2 cell, 5 somite and lateral and dorsal view of embryo head (D, L, P, T) or tail at 15 somite stage, respectively. Expression of ndst1a: A-D; ndst1b: E-H; ndst2a: I-L; ndst2b: M-P; ndst3: Q-T. Areas of the developing eye are marked with dotted lines. Abbreviations used: e, eye; f, forebrain; h, hindbrain; m, midbrain; nc, neural crest; s, somites; t, tectum.
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pone.0119040.g003: Expression of zebrafish ndst genes at early developmental stages.Lateral view of embryos at 2 cell, 5 somite and lateral and dorsal view of embryo head (D, L, P, T) or tail at 15 somite stage, respectively. Expression of ndst1a: A-D; ndst1b: E-H; ndst2a: I-L; ndst2b: M-P; ndst3: Q-T. Areas of the developing eye are marked with dotted lines. Abbreviations used: e, eye; f, forebrain; h, hindbrain; m, midbrain; nc, neural crest; s, somites; t, tectum.

Mentions: To study the distribution of the ndsts, in situ hybridization was performed at various embryonic stages, from the 2 cell stage until 3 days post fertilization (dpf), with probes specific for each of the five zebrafish ndst mRNAs (Table 1). All isoforms were maternally contributed as shown by staining and RT-PCR at the 2 cell stage (Fig. 3A, E, I, M, Q; S2 Fig.), a time point at which zygotic transcription has not yet commenced. All ndst genes showed distinct expression patterns, indicating that the in situ hybridization probes did not cross-hybridize with other ndst family members.


The NDST gene family in zebrafish: role of NDST1B in pharyngeal arch formation.

Filipek-Górniok B, Carlsson P, Haitina T, Habicher J, Ledin J, Kjellén L - PLoS ONE (2015)

Expression of zebrafish ndst genes at early developmental stages.Lateral view of embryos at 2 cell, 5 somite and lateral and dorsal view of embryo head (D, L, P, T) or tail at 15 somite stage, respectively. Expression of ndst1a: A-D; ndst1b: E-H; ndst2a: I-L; ndst2b: M-P; ndst3: Q-T. Areas of the developing eye are marked with dotted lines. Abbreviations used: e, eye; f, forebrain; h, hindbrain; m, midbrain; nc, neural crest; s, somites; t, tectum.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4359090&req=5

pone.0119040.g003: Expression of zebrafish ndst genes at early developmental stages.Lateral view of embryos at 2 cell, 5 somite and lateral and dorsal view of embryo head (D, L, P, T) or tail at 15 somite stage, respectively. Expression of ndst1a: A-D; ndst1b: E-H; ndst2a: I-L; ndst2b: M-P; ndst3: Q-T. Areas of the developing eye are marked with dotted lines. Abbreviations used: e, eye; f, forebrain; h, hindbrain; m, midbrain; nc, neural crest; s, somites; t, tectum.
Mentions: To study the distribution of the ndsts, in situ hybridization was performed at various embryonic stages, from the 2 cell stage until 3 days post fertilization (dpf), with probes specific for each of the five zebrafish ndst mRNAs (Table 1). All isoforms were maternally contributed as shown by staining and RT-PCR at the 2 cell stage (Fig. 3A, E, I, M, Q; S2 Fig.), a time point at which zygotic transcription has not yet commenced. All ndst genes showed distinct expression patterns, indicating that the in situ hybridization probes did not cross-hybridize with other ndst family members.

Bottom Line: Interestingly, the single zebrafish orthologue ndst3, is equally similar to tetrapod Ndst3 and Ndst4.It is likely that a local duplication in the common ancestor of lobe-finned fish and tetrapods gave rise to these two genes.All zebrafish Ndst genes showed distinct but partially overlapping expression patterns during embryonic development.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Medical Biochemistry and Microbiology, Science for Life Laboratory, Uppsala University, Husargatan 3, PO Box 582, SE-751 23, Uppsala, Sweden.

ABSTRACT
Heparan sulfate (HS) proteoglycans are ubiquitous components of the extracellular matrix and plasma membrane of metazoans. The sulfation pattern of the HS glycosaminoglycan chain is characteristic for each tissue and changes during development. The glucosaminyl N-deacetylase/N-sulfotransferase (NDST) enzymes catalyze N-deacetylation and N-sulfation during HS biosynthesis and have a key role in designing the sulfation pattern. We here report on the presence of five NDST genes in zebrafish. Zebrafish ndst1a, ndst1b, ndst2a and ndst2b represent duplicated mammalian orthologues of NDST1 and NDST2 that arose through teleost specific genome duplication. Interestingly, the single zebrafish orthologue ndst3, is equally similar to tetrapod Ndst3 and Ndst4. It is likely that a local duplication in the common ancestor of lobe-finned fish and tetrapods gave rise to these two genes. All zebrafish Ndst genes showed distinct but partially overlapping expression patterns during embryonic development. Morpholino knockdown of ndst1b resulted in delayed development, craniofacial cartilage abnormalities, shortened body and pectoral fin length, resembling some of the features of the Ndst1 mouse knockout.

Show MeSH
Related in: MedlinePlus