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The NDST gene family in zebrafish: role of NDST1B in pharyngeal arch formation.

Filipek-Górniok B, Carlsson P, Haitina T, Habicher J, Ledin J, Kjellén L - PLoS ONE (2015)

Bottom Line: Interestingly, the single zebrafish orthologue ndst3, is equally similar to tetrapod Ndst3 and Ndst4.It is likely that a local duplication in the common ancestor of lobe-finned fish and tetrapods gave rise to these two genes.All zebrafish Ndst genes showed distinct but partially overlapping expression patterns during embryonic development.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Medical Biochemistry and Microbiology, Science for Life Laboratory, Uppsala University, Husargatan 3, PO Box 582, SE-751 23, Uppsala, Sweden.

ABSTRACT
Heparan sulfate (HS) proteoglycans are ubiquitous components of the extracellular matrix and plasma membrane of metazoans. The sulfation pattern of the HS glycosaminoglycan chain is characteristic for each tissue and changes during development. The glucosaminyl N-deacetylase/N-sulfotransferase (NDST) enzymes catalyze N-deacetylation and N-sulfation during HS biosynthesis and have a key role in designing the sulfation pattern. We here report on the presence of five NDST genes in zebrafish. Zebrafish ndst1a, ndst1b, ndst2a and ndst2b represent duplicated mammalian orthologues of NDST1 and NDST2 that arose through teleost specific genome duplication. Interestingly, the single zebrafish orthologue ndst3, is equally similar to tetrapod Ndst3 and Ndst4. It is likely that a local duplication in the common ancestor of lobe-finned fish and tetrapods gave rise to these two genes. All zebrafish Ndst genes showed distinct but partially overlapping expression patterns during embryonic development. Morpholino knockdown of ndst1b resulted in delayed development, craniofacial cartilage abnormalities, shortened body and pectoral fin length, resembling some of the features of the Ndst1 mouse knockout.

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Schematic presentation of chromosomal or scaffold position of NDST3, NDST4, UGT8 and PRSS12 in different vertebrate species.Arrowed rounded rectangles display the orientation of the genes. Numbers between the genes indicate the distances in kilo base pairs. Gene name abbreviations: UGT8 (ceramide UDP-galactosyltransferase), PRSS12 (neurotrypsin).
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pone.0119040.g002: Schematic presentation of chromosomal or scaffold position of NDST3, NDST4, UGT8 and PRSS12 in different vertebrate species.Arrowed rounded rectangles display the orientation of the genes. Numbers between the genes indicate the distances in kilo base pairs. Gene name abbreviations: UGT8 (ceramide UDP-galactosyltransferase), PRSS12 (neurotrypsin).

Mentions: To elucidate the relation of the single zebrafish orthologue to NDST3 and NDST4, we analyzed NDST3 and NDST4 localization and orientation together with the neighboring genes encoding ceramide UDP-galactosyltransferase (UGT8) and neurotrypsin (PRSS12) in different genomes (Fig. 2). Notably, in vertebrates NDST3 and NDST4 always have opposite orientation, where NDST3 is facing PRSS12 and NDST4 is facing UGT8 with 20–150kb between their 3'-ends (Fig. 2). In tetrapods, NDST3 and NDST4 subtypes share around 80% identity in amino acid sequence and are more similar to each other than to other NDST subtypes.


The NDST gene family in zebrafish: role of NDST1B in pharyngeal arch formation.

Filipek-Górniok B, Carlsson P, Haitina T, Habicher J, Ledin J, Kjellén L - PLoS ONE (2015)

Schematic presentation of chromosomal or scaffold position of NDST3, NDST4, UGT8 and PRSS12 in different vertebrate species.Arrowed rounded rectangles display the orientation of the genes. Numbers between the genes indicate the distances in kilo base pairs. Gene name abbreviations: UGT8 (ceramide UDP-galactosyltransferase), PRSS12 (neurotrypsin).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4359090&req=5

pone.0119040.g002: Schematic presentation of chromosomal or scaffold position of NDST3, NDST4, UGT8 and PRSS12 in different vertebrate species.Arrowed rounded rectangles display the orientation of the genes. Numbers between the genes indicate the distances in kilo base pairs. Gene name abbreviations: UGT8 (ceramide UDP-galactosyltransferase), PRSS12 (neurotrypsin).
Mentions: To elucidate the relation of the single zebrafish orthologue to NDST3 and NDST4, we analyzed NDST3 and NDST4 localization and orientation together with the neighboring genes encoding ceramide UDP-galactosyltransferase (UGT8) and neurotrypsin (PRSS12) in different genomes (Fig. 2). Notably, in vertebrates NDST3 and NDST4 always have opposite orientation, where NDST3 is facing PRSS12 and NDST4 is facing UGT8 with 20–150kb between their 3'-ends (Fig. 2). In tetrapods, NDST3 and NDST4 subtypes share around 80% identity in amino acid sequence and are more similar to each other than to other NDST subtypes.

Bottom Line: Interestingly, the single zebrafish orthologue ndst3, is equally similar to tetrapod Ndst3 and Ndst4.It is likely that a local duplication in the common ancestor of lobe-finned fish and tetrapods gave rise to these two genes.All zebrafish Ndst genes showed distinct but partially overlapping expression patterns during embryonic development.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Medical Biochemistry and Microbiology, Science for Life Laboratory, Uppsala University, Husargatan 3, PO Box 582, SE-751 23, Uppsala, Sweden.

ABSTRACT
Heparan sulfate (HS) proteoglycans are ubiquitous components of the extracellular matrix and plasma membrane of metazoans. The sulfation pattern of the HS glycosaminoglycan chain is characteristic for each tissue and changes during development. The glucosaminyl N-deacetylase/N-sulfotransferase (NDST) enzymes catalyze N-deacetylation and N-sulfation during HS biosynthesis and have a key role in designing the sulfation pattern. We here report on the presence of five NDST genes in zebrafish. Zebrafish ndst1a, ndst1b, ndst2a and ndst2b represent duplicated mammalian orthologues of NDST1 and NDST2 that arose through teleost specific genome duplication. Interestingly, the single zebrafish orthologue ndst3, is equally similar to tetrapod Ndst3 and Ndst4. It is likely that a local duplication in the common ancestor of lobe-finned fish and tetrapods gave rise to these two genes. All zebrafish Ndst genes showed distinct but partially overlapping expression patterns during embryonic development. Morpholino knockdown of ndst1b resulted in delayed development, craniofacial cartilage abnormalities, shortened body and pectoral fin length, resembling some of the features of the Ndst1 mouse knockout.

Show MeSH
Related in: MedlinePlus