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Enhanced chloroplastic generation of H2O2 in stress-resistant Thellungiella salsuginea in comparison to Arabidopsis thaliana.

Wiciarz M, Gubernator B, Kruk J, Niewiadomska E - Physiol Plant (2014)

Bottom Line: In order to find some basis of salinity resistance in the chloroplastic metabolism, a halophytic Thellungiella salsuginea was compared with glycophytic Arabidopsis thaliana.Salinity treatment (0.15 and 0.30 M NaCl for A.t. and T.s., respectively) led to a decrease in ratios of chl a/b and F730 /F685 .In A.t., a salinity-driven enhancement of YII and NPQ was found, in association with the stimulation of H2 O2 production from PQ pool.

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Affiliation: Department of Plant Physiology and Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30-387, Kraków, Poland.

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Rates of H2O2 production by illuminated thylakoids from Arabidopsis thaliana and Thellungiella salsuginea plants irrigated with water or with NaCl solution. Salinity of 0.15 and 0.3 M NaCl was used for A.t. and T.s., respectively. Data represent mean ± sd (n ≥ 5). ‘Diamond’ indicates a significant difference between control and salinity-treated plants. ‘Asterisk’ indicates a significant difference from A.t. from the same treatment.
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fig04: Rates of H2O2 production by illuminated thylakoids from Arabidopsis thaliana and Thellungiella salsuginea plants irrigated with water or with NaCl solution. Salinity of 0.15 and 0.3 M NaCl was used for A.t. and T.s., respectively. Data represent mean ± sd (n ≥ 5). ‘Diamond’ indicates a significant difference between control and salinity-treated plants. ‘Asterisk’ indicates a significant difference from A.t. from the same treatment.

Mentions: To compare a whole chain electron transport in the two species we followed the H2O2 production by illuminated thylakoids in the absence of artificial electron donors and acceptors. This approach was chosen instead of the typical method, based on the rate of O2 uptake, to avoid a possible error which might be caused by the oxygen-consuming activity of PTOX, because literature data (Stepien and Johnson 2009) points to the increased participation of chlororespiration in T.s. Thylakoids isolated from control T.s. plants were characterized by about 2.3 times more intensive H2O2 production, in comparison to A.t. (Fig. 4). After salinity treatment an enhancement of H2O2 production was noted in both species, however it was significant only in A.t.


Enhanced chloroplastic generation of H2O2 in stress-resistant Thellungiella salsuginea in comparison to Arabidopsis thaliana.

Wiciarz M, Gubernator B, Kruk J, Niewiadomska E - Physiol Plant (2014)

Rates of H2O2 production by illuminated thylakoids from Arabidopsis thaliana and Thellungiella salsuginea plants irrigated with water or with NaCl solution. Salinity of 0.15 and 0.3 M NaCl was used for A.t. and T.s., respectively. Data represent mean ± sd (n ≥ 5). ‘Diamond’ indicates a significant difference between control and salinity-treated plants. ‘Asterisk’ indicates a significant difference from A.t. from the same treatment.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4359041&req=5

fig04: Rates of H2O2 production by illuminated thylakoids from Arabidopsis thaliana and Thellungiella salsuginea plants irrigated with water or with NaCl solution. Salinity of 0.15 and 0.3 M NaCl was used for A.t. and T.s., respectively. Data represent mean ± sd (n ≥ 5). ‘Diamond’ indicates a significant difference between control and salinity-treated plants. ‘Asterisk’ indicates a significant difference from A.t. from the same treatment.
Mentions: To compare a whole chain electron transport in the two species we followed the H2O2 production by illuminated thylakoids in the absence of artificial electron donors and acceptors. This approach was chosen instead of the typical method, based on the rate of O2 uptake, to avoid a possible error which might be caused by the oxygen-consuming activity of PTOX, because literature data (Stepien and Johnson 2009) points to the increased participation of chlororespiration in T.s. Thylakoids isolated from control T.s. plants were characterized by about 2.3 times more intensive H2O2 production, in comparison to A.t. (Fig. 4). After salinity treatment an enhancement of H2O2 production was noted in both species, however it was significant only in A.t.

Bottom Line: In order to find some basis of salinity resistance in the chloroplastic metabolism, a halophytic Thellungiella salsuginea was compared with glycophytic Arabidopsis thaliana.Salinity treatment (0.15 and 0.30 M NaCl for A.t. and T.s., respectively) led to a decrease in ratios of chl a/b and F730 /F685 .In A.t., a salinity-driven enhancement of YII and NPQ was found, in association with the stimulation of H2 O2 production from PQ pool.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Physiology and Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30-387, Kraków, Poland.

Show MeSH
Related in: MedlinePlus