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Enhanced chloroplastic generation of H2O2 in stress-resistant Thellungiella salsuginea in comparison to Arabidopsis thaliana.

Wiciarz M, Gubernator B, Kruk J, Niewiadomska E - Physiol Plant (2014)

Bottom Line: In order to find some basis of salinity resistance in the chloroplastic metabolism, a halophytic Thellungiella salsuginea was compared with glycophytic Arabidopsis thaliana.Salinity treatment (0.15 and 0.30 M NaCl for A.t. and T.s., respectively) led to a decrease in ratios of chl a/b and F730 /F685 .In A.t., a salinity-driven enhancement of YII and NPQ was found, in association with the stimulation of H2 O2 production from PQ pool.

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Affiliation: Department of Plant Physiology and Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30-387, Kraków, Poland.

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A comparison of PSI activities measured with oxygen electrode (A) and with H2O2 electrode (B). Thylakoids were isolated from leaves of Arabidopsis thaliana and Thellungiella salsuginea plants irrigated with water and with NaCl solution. Salinity of 0.15 and 0.3 M NaCl was used for A.t. and T.s., respectively. Data represent mean ± sd (n = 4). ‘Diamond’ indicates a significant difference between control and salinity-treated plants. ‘Asterisk’ indicates a significant difference from A.t. from the same treatment.
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fig03: A comparison of PSI activities measured with oxygen electrode (A) and with H2O2 electrode (B). Thylakoids were isolated from leaves of Arabidopsis thaliana and Thellungiella salsuginea plants irrigated with water and with NaCl solution. Salinity of 0.15 and 0.3 M NaCl was used for A.t. and T.s., respectively. Data represent mean ± sd (n = 4). ‘Diamond’ indicates a significant difference between control and salinity-treated plants. ‘Asterisk’ indicates a significant difference from A.t. from the same treatment.

Mentions: Changes in PSI activity were detected by mean of oxygen consumption by isolated thylakoids supplied with electron donors (DCPiP and AsA) and acceptor (MV) at light intensity of 400 µmol quanta m−2 s−1 (Fig. 3). This light intensity was chosen after checking the light dependence of PSI activity (Fig. S1) in order to maximize PET and, at the same time, to avoid photoinhibition. In thylakoids isolated from control Thellungiella plants activity of PSI was 27% higher than in thylakoids from Arabidopsis. Salinity treatment strongly decreased PSI activity in Arabidopsis (by about 50%), while in T.s. only a slight reduction (by 7%) was noted.


Enhanced chloroplastic generation of H2O2 in stress-resistant Thellungiella salsuginea in comparison to Arabidopsis thaliana.

Wiciarz M, Gubernator B, Kruk J, Niewiadomska E - Physiol Plant (2014)

A comparison of PSI activities measured with oxygen electrode (A) and with H2O2 electrode (B). Thylakoids were isolated from leaves of Arabidopsis thaliana and Thellungiella salsuginea plants irrigated with water and with NaCl solution. Salinity of 0.15 and 0.3 M NaCl was used for A.t. and T.s., respectively. Data represent mean ± sd (n = 4). ‘Diamond’ indicates a significant difference between control and salinity-treated plants. ‘Asterisk’ indicates a significant difference from A.t. from the same treatment.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4359041&req=5

fig03: A comparison of PSI activities measured with oxygen electrode (A) and with H2O2 electrode (B). Thylakoids were isolated from leaves of Arabidopsis thaliana and Thellungiella salsuginea plants irrigated with water and with NaCl solution. Salinity of 0.15 and 0.3 M NaCl was used for A.t. and T.s., respectively. Data represent mean ± sd (n = 4). ‘Diamond’ indicates a significant difference between control and salinity-treated plants. ‘Asterisk’ indicates a significant difference from A.t. from the same treatment.
Mentions: Changes in PSI activity were detected by mean of oxygen consumption by isolated thylakoids supplied with electron donors (DCPiP and AsA) and acceptor (MV) at light intensity of 400 µmol quanta m−2 s−1 (Fig. 3). This light intensity was chosen after checking the light dependence of PSI activity (Fig. S1) in order to maximize PET and, at the same time, to avoid photoinhibition. In thylakoids isolated from control Thellungiella plants activity of PSI was 27% higher than in thylakoids from Arabidopsis. Salinity treatment strongly decreased PSI activity in Arabidopsis (by about 50%), while in T.s. only a slight reduction (by 7%) was noted.

Bottom Line: In order to find some basis of salinity resistance in the chloroplastic metabolism, a halophytic Thellungiella salsuginea was compared with glycophytic Arabidopsis thaliana.Salinity treatment (0.15 and 0.30 M NaCl for A.t. and T.s., respectively) led to a decrease in ratios of chl a/b and F730 /F685 .In A.t., a salinity-driven enhancement of YII and NPQ was found, in association with the stimulation of H2 O2 production from PQ pool.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Physiology and Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30-387, Kraków, Poland.

Show MeSH
Related in: MedlinePlus