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Favorable alleles for stem water-soluble carbohydrates identified by association analysis contribute to grain weight under drought stress conditions in wheat.

Li W, Zhang B, Li R, Chang X, Jing R - PLoS ONE (2015)

Bottom Line: Sixteen novel WSC-favorable alleles were identified, and five of them contributed to significantly higher TGW.In addition, pyramiding WSC favorable alleles was not only effective for obtaining accessions with higher WSC, but also for enhancing TGW under different water regimes.The results indicate a high potential for using marker-assisted selection to pyramid WSC favorable alleles in improving WSC and TGW in wheat.

View Article: PubMed Central - PubMed

Affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement / Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; Agronomy College, Shanxi Agricultural University, Taigu 030801, China.

ABSTRACT
Drought is a major environmental constraint to crop distribution and productivity. Stem water-soluble carbohydrates (WSC) buffer wheat grain yield against conditions unfavorable for photosynthesis during the grain filling stage. In this study, 262 winter wheat accessions and 209 genome-wide SSR markers were collected and used to undertake association analysis based on a mixed linear model (MLM). The WSC in different internodes at three growth stages and 1000-grain weight (TGW) were investigated under four environmental regimes (well-watered, drought stress during the whole growth period, and two levels of terminal drought stress imposed by chemical desiccation under the well-watered and drought stress during the whole growth period conditions). Under diverse drought stress conditions, WSC in lower internodes showed significant positive correlations with TGW, especially at the flowering stage under well-watered conditions and at grain filling under drought stress. Sixteen novel WSC-favorable alleles were identified, and five of them contributed to significantly higher TGW. In addition, pyramiding WSC favorable alleles was not only effective for obtaining accessions with higher WSC, but also for enhancing TGW under different water regimes. During the past fifty years of wheat breeding, WSC was selected incidentally. The average number of favorable WSC alleles increased from 1.13 in the pre-1960 period to 4.41 in the post-2000 period. The results indicate a high potential for using marker-assisted selection to pyramid WSC favorable alleles in improving WSC and TGW in wheat.

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Single WSC favorable allele contributed to significantly higher 1000-grain weight (TGW).Bars indicate 2×SE. WSC corresponding to Xbarc181–1B, Xgwm261–2D and Xgwm149–4B represent WSC at the mid-grain filling under CK-DS, KI-DS and KI-DS, respectively; WSC corresponding to Xgwm148–2B and Xgwm358–5D represent WSC at flowering under CK-WW. TGW was measured at maturity. *, ** indicate significance at P = 0.05 and P = 0.01, respectively.
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pone.0119438.g005: Single WSC favorable allele contributed to significantly higher 1000-grain weight (TGW).Bars indicate 2×SE. WSC corresponding to Xbarc181–1B, Xgwm261–2D and Xgwm149–4B represent WSC at the mid-grain filling under CK-DS, KI-DS and KI-DS, respectively; WSC corresponding to Xgwm148–2B and Xgwm358–5D represent WSC at flowering under CK-WW. TGW was measured at maturity. *, ** indicate significance at P = 0.05 and P = 0.01, respectively.

Mentions: In accordance with the significant positive correlations between WSC and TGW, the TGW of accessions carrying favorable WSC alleles were overall much higher than those of others. Only five of the 16 favorable WSC alleles, individually contributed to significantly higher TGW; they were Xbarc181–1B187, Xgwm148–2B165, Xgwm261–2D203, Xgwm149–4B153 and Xgwm358–5D162 (Fig. 5). We also analyzed the TGW of accessions containing different numbers of favorable WSC alleles under diverse water conditions (CK-WW, CK-DS, KI-WW, KI-DS, Table 3). Under CK-DS, accessions combining more than two favorable WSC alleles had significantly (P <0.05) higher TGW (43.75 g) than those without favorable alleles (41.21 g). Except for KI-WW, the pyramiding of favorable WSC alleles was also effective under CK-WW and KI-DS. There were only 4 accessions carrying 2 favorable alleles where significant differences under KI-WW were not detected.


Favorable alleles for stem water-soluble carbohydrates identified by association analysis contribute to grain weight under drought stress conditions in wheat.

Li W, Zhang B, Li R, Chang X, Jing R - PLoS ONE (2015)

Single WSC favorable allele contributed to significantly higher 1000-grain weight (TGW).Bars indicate 2×SE. WSC corresponding to Xbarc181–1B, Xgwm261–2D and Xgwm149–4B represent WSC at the mid-grain filling under CK-DS, KI-DS and KI-DS, respectively; WSC corresponding to Xgwm148–2B and Xgwm358–5D represent WSC at flowering under CK-WW. TGW was measured at maturity. *, ** indicate significance at P = 0.05 and P = 0.01, respectively.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4358973&req=5

pone.0119438.g005: Single WSC favorable allele contributed to significantly higher 1000-grain weight (TGW).Bars indicate 2×SE. WSC corresponding to Xbarc181–1B, Xgwm261–2D and Xgwm149–4B represent WSC at the mid-grain filling under CK-DS, KI-DS and KI-DS, respectively; WSC corresponding to Xgwm148–2B and Xgwm358–5D represent WSC at flowering under CK-WW. TGW was measured at maturity. *, ** indicate significance at P = 0.05 and P = 0.01, respectively.
Mentions: In accordance with the significant positive correlations between WSC and TGW, the TGW of accessions carrying favorable WSC alleles were overall much higher than those of others. Only five of the 16 favorable WSC alleles, individually contributed to significantly higher TGW; they were Xbarc181–1B187, Xgwm148–2B165, Xgwm261–2D203, Xgwm149–4B153 and Xgwm358–5D162 (Fig. 5). We also analyzed the TGW of accessions containing different numbers of favorable WSC alleles under diverse water conditions (CK-WW, CK-DS, KI-WW, KI-DS, Table 3). Under CK-DS, accessions combining more than two favorable WSC alleles had significantly (P <0.05) higher TGW (43.75 g) than those without favorable alleles (41.21 g). Except for KI-WW, the pyramiding of favorable WSC alleles was also effective under CK-WW and KI-DS. There were only 4 accessions carrying 2 favorable alleles where significant differences under KI-WW were not detected.

Bottom Line: Sixteen novel WSC-favorable alleles were identified, and five of them contributed to significantly higher TGW.In addition, pyramiding WSC favorable alleles was not only effective for obtaining accessions with higher WSC, but also for enhancing TGW under different water regimes.The results indicate a high potential for using marker-assisted selection to pyramid WSC favorable alleles in improving WSC and TGW in wheat.

View Article: PubMed Central - PubMed

Affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement / Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; Agronomy College, Shanxi Agricultural University, Taigu 030801, China.

ABSTRACT
Drought is a major environmental constraint to crop distribution and productivity. Stem water-soluble carbohydrates (WSC) buffer wheat grain yield against conditions unfavorable for photosynthesis during the grain filling stage. In this study, 262 winter wheat accessions and 209 genome-wide SSR markers were collected and used to undertake association analysis based on a mixed linear model (MLM). The WSC in different internodes at three growth stages and 1000-grain weight (TGW) were investigated under four environmental regimes (well-watered, drought stress during the whole growth period, and two levels of terminal drought stress imposed by chemical desiccation under the well-watered and drought stress during the whole growth period conditions). Under diverse drought stress conditions, WSC in lower internodes showed significant positive correlations with TGW, especially at the flowering stage under well-watered conditions and at grain filling under drought stress. Sixteen novel WSC-favorable alleles were identified, and five of them contributed to significantly higher TGW. In addition, pyramiding WSC favorable alleles was not only effective for obtaining accessions with higher WSC, but also for enhancing TGW under different water regimes. During the past fifty years of wheat breeding, WSC was selected incidentally. The average number of favorable WSC alleles increased from 1.13 in the pre-1960 period to 4.41 in the post-2000 period. The results indicate a high potential for using marker-assisted selection to pyramid WSC favorable alleles in improving WSC and TGW in wheat.

Show MeSH