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Favorable alleles for stem water-soluble carbohydrates identified by association analysis contribute to grain weight under drought stress conditions in wheat.

Li W, Zhang B, Li R, Chang X, Jing R - PLoS ONE (2015)

Bottom Line: Sixteen novel WSC-favorable alleles were identified, and five of them contributed to significantly higher TGW.In addition, pyramiding WSC favorable alleles was not only effective for obtaining accessions with higher WSC, but also for enhancing TGW under different water regimes.The results indicate a high potential for using marker-assisted selection to pyramid WSC favorable alleles in improving WSC and TGW in wheat.

View Article: PubMed Central - PubMed

Affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement / Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; Agronomy College, Shanxi Agricultural University, Taigu 030801, China.

ABSTRACT
Drought is a major environmental constraint to crop distribution and productivity. Stem water-soluble carbohydrates (WSC) buffer wheat grain yield against conditions unfavorable for photosynthesis during the grain filling stage. In this study, 262 winter wheat accessions and 209 genome-wide SSR markers were collected and used to undertake association analysis based on a mixed linear model (MLM). The WSC in different internodes at three growth stages and 1000-grain weight (TGW) were investigated under four environmental regimes (well-watered, drought stress during the whole growth period, and two levels of terminal drought stress imposed by chemical desiccation under the well-watered and drought stress during the whole growth period conditions). Under diverse drought stress conditions, WSC in lower internodes showed significant positive correlations with TGW, especially at the flowering stage under well-watered conditions and at grain filling under drought stress. Sixteen novel WSC-favorable alleles were identified, and five of them contributed to significantly higher TGW. In addition, pyramiding WSC favorable alleles was not only effective for obtaining accessions with higher WSC, but also for enhancing TGW under different water regimes. During the past fifty years of wheat breeding, WSC was selected incidentally. The average number of favorable WSC alleles increased from 1.13 in the pre-1960 period to 4.41 in the post-2000 period. The results indicate a high potential for using marker-assisted selection to pyramid WSC favorable alleles in improving WSC and TGW in wheat.

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Principal coordinate analysis of 262 wheat accessions based on 209 SSR markers reveals two sub-populations.
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pone.0119438.g004: Principal coordinate analysis of 262 wheat accessions based on 209 SSR markers reveals two sub-populations.

Mentions: A total of 2,748 alleles were identified at the 209 SSR loci in our population (262 accessions), an average of 13/locus. The average percentage of missing data for all markers and genotypes was 3.36%. The polymorphism information content (PIC) of the SSR loci ranged from 0.0175 to 0.932 with an average of 0.619. Population structure was assessed by STRUCTURE v2.3.2 using all markers. The most significant change of the LnP(D) value was observed at k = 2, and there was also a sharp peak of △k, indicating that k = 2 was the best separator (Fig. 3). This was further supported by principal coordinate analysis based on Nei’s genetic distances [38]. The top two principal components clearly separated the 262 wheat accessions into two sub-populations, comprising 126 and 136 accessions, respectively, and agreeing the with the STRUCTURE analysis (Fig. 4).


Favorable alleles for stem water-soluble carbohydrates identified by association analysis contribute to grain weight under drought stress conditions in wheat.

Li W, Zhang B, Li R, Chang X, Jing R - PLoS ONE (2015)

Principal coordinate analysis of 262 wheat accessions based on 209 SSR markers reveals two sub-populations.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4358973&req=5

pone.0119438.g004: Principal coordinate analysis of 262 wheat accessions based on 209 SSR markers reveals two sub-populations.
Mentions: A total of 2,748 alleles were identified at the 209 SSR loci in our population (262 accessions), an average of 13/locus. The average percentage of missing data for all markers and genotypes was 3.36%. The polymorphism information content (PIC) of the SSR loci ranged from 0.0175 to 0.932 with an average of 0.619. Population structure was assessed by STRUCTURE v2.3.2 using all markers. The most significant change of the LnP(D) value was observed at k = 2, and there was also a sharp peak of △k, indicating that k = 2 was the best separator (Fig. 3). This was further supported by principal coordinate analysis based on Nei’s genetic distances [38]. The top two principal components clearly separated the 262 wheat accessions into two sub-populations, comprising 126 and 136 accessions, respectively, and agreeing the with the STRUCTURE analysis (Fig. 4).

Bottom Line: Sixteen novel WSC-favorable alleles were identified, and five of them contributed to significantly higher TGW.In addition, pyramiding WSC favorable alleles was not only effective for obtaining accessions with higher WSC, but also for enhancing TGW under different water regimes.The results indicate a high potential for using marker-assisted selection to pyramid WSC favorable alleles in improving WSC and TGW in wheat.

View Article: PubMed Central - PubMed

Affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement / Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; Agronomy College, Shanxi Agricultural University, Taigu 030801, China.

ABSTRACT
Drought is a major environmental constraint to crop distribution and productivity. Stem water-soluble carbohydrates (WSC) buffer wheat grain yield against conditions unfavorable for photosynthesis during the grain filling stage. In this study, 262 winter wheat accessions and 209 genome-wide SSR markers were collected and used to undertake association analysis based on a mixed linear model (MLM). The WSC in different internodes at three growth stages and 1000-grain weight (TGW) were investigated under four environmental regimes (well-watered, drought stress during the whole growth period, and two levels of terminal drought stress imposed by chemical desiccation under the well-watered and drought stress during the whole growth period conditions). Under diverse drought stress conditions, WSC in lower internodes showed significant positive correlations with TGW, especially at the flowering stage under well-watered conditions and at grain filling under drought stress. Sixteen novel WSC-favorable alleles were identified, and five of them contributed to significantly higher TGW. In addition, pyramiding WSC favorable alleles was not only effective for obtaining accessions with higher WSC, but also for enhancing TGW under different water regimes. During the past fifty years of wheat breeding, WSC was selected incidentally. The average number of favorable WSC alleles increased from 1.13 in the pre-1960 period to 4.41 in the post-2000 period. The results indicate a high potential for using marker-assisted selection to pyramid WSC favorable alleles in improving WSC and TGW in wheat.

Show MeSH