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Favorable alleles for stem water-soluble carbohydrates identified by association analysis contribute to grain weight under drought stress conditions in wheat.

Li W, Zhang B, Li R, Chang X, Jing R - PLoS ONE (2015)

Bottom Line: Sixteen novel WSC-favorable alleles were identified, and five of them contributed to significantly higher TGW.In addition, pyramiding WSC favorable alleles was not only effective for obtaining accessions with higher WSC, but also for enhancing TGW under different water regimes.The results indicate a high potential for using marker-assisted selection to pyramid WSC favorable alleles in improving WSC and TGW in wheat.

View Article: PubMed Central - PubMed

Affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement / Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; Agronomy College, Shanxi Agricultural University, Taigu 030801, China.

ABSTRACT
Drought is a major environmental constraint to crop distribution and productivity. Stem water-soluble carbohydrates (WSC) buffer wheat grain yield against conditions unfavorable for photosynthesis during the grain filling stage. In this study, 262 winter wheat accessions and 209 genome-wide SSR markers were collected and used to undertake association analysis based on a mixed linear model (MLM). The WSC in different internodes at three growth stages and 1000-grain weight (TGW) were investigated under four environmental regimes (well-watered, drought stress during the whole growth period, and two levels of terminal drought stress imposed by chemical desiccation under the well-watered and drought stress during the whole growth period conditions). Under diverse drought stress conditions, WSC in lower internodes showed significant positive correlations with TGW, especially at the flowering stage under well-watered conditions and at grain filling under drought stress. Sixteen novel WSC-favorable alleles were identified, and five of them contributed to significantly higher TGW. In addition, pyramiding WSC favorable alleles was not only effective for obtaining accessions with higher WSC, but also for enhancing TGW under different water regimes. During the past fifty years of wheat breeding, WSC was selected incidentally. The average number of favorable WSC alleles increased from 1.13 in the pre-1960 period to 4.41 in the post-2000 period. The results indicate a high potential for using marker-assisted selection to pyramid WSC favorable alleles in improving WSC and TGW in wheat.

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Stem water-soluble carbohydrates (WSC, mg / g dw) of 262 winter wheat accessions in different internodes at three growth stages under well-watered and drought stress environments.Bars indicate 2×SE. CK-DS-Ped, peduncle under drought stress; CK-DS-Low, lower internode, drought stressed; CK-DS-Ste, whole stem, drought stressed; CK-WW-Ped, peduncle, well-watered; CK-WW-Low, lower internode, well-watered; CK-WW-Ste, whole stem, well-watered; KI-DS-Ped, peduncle treated with KI, drought stressed; KI-DS-Low, lower internode treated with KI, drought stressed; KI-DS-Ste, whole stem treated with KI, drought stressed; KI-WW-Ped, peduncle treated with KI, well-watered; KI-WW-Low, lower internode treated with KI, well-watered; KI-WW-Ste, whole stem treated with KI, well-watered.
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pone.0119438.g001: Stem water-soluble carbohydrates (WSC, mg / g dw) of 262 winter wheat accessions in different internodes at three growth stages under well-watered and drought stress environments.Bars indicate 2×SE. CK-DS-Ped, peduncle under drought stress; CK-DS-Low, lower internode, drought stressed; CK-DS-Ste, whole stem, drought stressed; CK-WW-Ped, peduncle, well-watered; CK-WW-Low, lower internode, well-watered; CK-WW-Ste, whole stem, well-watered; KI-DS-Ped, peduncle treated with KI, drought stressed; KI-DS-Low, lower internode treated with KI, drought stressed; KI-DS-Ste, whole stem treated with KI, drought stressed; KI-WW-Ped, peduncle treated with KI, well-watered; KI-WW-Low, lower internode treated with KI, well-watered; KI-WW-Ste, whole stem treated with KI, well-watered.

Mentions: A total of 262 common wheat accessions with similar flowering time were collected as a diverse population for our research. The average values of 262 wheat accessions for WSC, TGW and grain-filling efficiency (GFE) were presented in S2 and S3 Tables, Fig. 1 and Fig. 2. There was considerable phenotypic variability for WSC and TGW in the natural population with coefficients of variation (CV) ranging from 16.11 to 50.51% and from 14.65 to 43.64%, respectively. The WSC at the mid-grain filling were higher than those at flowering and maturity stages. Before maturity, the WSC was overall much higher in lower internodes than that in the peduncles in every water regime (Fig. 1; S2 Table).


Favorable alleles for stem water-soluble carbohydrates identified by association analysis contribute to grain weight under drought stress conditions in wheat.

Li W, Zhang B, Li R, Chang X, Jing R - PLoS ONE (2015)

Stem water-soluble carbohydrates (WSC, mg / g dw) of 262 winter wheat accessions in different internodes at three growth stages under well-watered and drought stress environments.Bars indicate 2×SE. CK-DS-Ped, peduncle under drought stress; CK-DS-Low, lower internode, drought stressed; CK-DS-Ste, whole stem, drought stressed; CK-WW-Ped, peduncle, well-watered; CK-WW-Low, lower internode, well-watered; CK-WW-Ste, whole stem, well-watered; KI-DS-Ped, peduncle treated with KI, drought stressed; KI-DS-Low, lower internode treated with KI, drought stressed; KI-DS-Ste, whole stem treated with KI, drought stressed; KI-WW-Ped, peduncle treated with KI, well-watered; KI-WW-Low, lower internode treated with KI, well-watered; KI-WW-Ste, whole stem treated with KI, well-watered.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4358973&req=5

pone.0119438.g001: Stem water-soluble carbohydrates (WSC, mg / g dw) of 262 winter wheat accessions in different internodes at three growth stages under well-watered and drought stress environments.Bars indicate 2×SE. CK-DS-Ped, peduncle under drought stress; CK-DS-Low, lower internode, drought stressed; CK-DS-Ste, whole stem, drought stressed; CK-WW-Ped, peduncle, well-watered; CK-WW-Low, lower internode, well-watered; CK-WW-Ste, whole stem, well-watered; KI-DS-Ped, peduncle treated with KI, drought stressed; KI-DS-Low, lower internode treated with KI, drought stressed; KI-DS-Ste, whole stem treated with KI, drought stressed; KI-WW-Ped, peduncle treated with KI, well-watered; KI-WW-Low, lower internode treated with KI, well-watered; KI-WW-Ste, whole stem treated with KI, well-watered.
Mentions: A total of 262 common wheat accessions with similar flowering time were collected as a diverse population for our research. The average values of 262 wheat accessions for WSC, TGW and grain-filling efficiency (GFE) were presented in S2 and S3 Tables, Fig. 1 and Fig. 2. There was considerable phenotypic variability for WSC and TGW in the natural population with coefficients of variation (CV) ranging from 16.11 to 50.51% and from 14.65 to 43.64%, respectively. The WSC at the mid-grain filling were higher than those at flowering and maturity stages. Before maturity, the WSC was overall much higher in lower internodes than that in the peduncles in every water regime (Fig. 1; S2 Table).

Bottom Line: Sixteen novel WSC-favorable alleles were identified, and five of them contributed to significantly higher TGW.In addition, pyramiding WSC favorable alleles was not only effective for obtaining accessions with higher WSC, but also for enhancing TGW under different water regimes.The results indicate a high potential for using marker-assisted selection to pyramid WSC favorable alleles in improving WSC and TGW in wheat.

View Article: PubMed Central - PubMed

Affiliation: National Key Facility for Crop Gene Resources and Genetic Improvement / Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; Agronomy College, Shanxi Agricultural University, Taigu 030801, China.

ABSTRACT
Drought is a major environmental constraint to crop distribution and productivity. Stem water-soluble carbohydrates (WSC) buffer wheat grain yield against conditions unfavorable for photosynthesis during the grain filling stage. In this study, 262 winter wheat accessions and 209 genome-wide SSR markers were collected and used to undertake association analysis based on a mixed linear model (MLM). The WSC in different internodes at three growth stages and 1000-grain weight (TGW) were investigated under four environmental regimes (well-watered, drought stress during the whole growth period, and two levels of terminal drought stress imposed by chemical desiccation under the well-watered and drought stress during the whole growth period conditions). Under diverse drought stress conditions, WSC in lower internodes showed significant positive correlations with TGW, especially at the flowering stage under well-watered conditions and at grain filling under drought stress. Sixteen novel WSC-favorable alleles were identified, and five of them contributed to significantly higher TGW. In addition, pyramiding WSC favorable alleles was not only effective for obtaining accessions with higher WSC, but also for enhancing TGW under different water regimes. During the past fifty years of wheat breeding, WSC was selected incidentally. The average number of favorable WSC alleles increased from 1.13 in the pre-1960 period to 4.41 in the post-2000 period. The results indicate a high potential for using marker-assisted selection to pyramid WSC favorable alleles in improving WSC and TGW in wheat.

Show MeSH